Analysis of the three dimensional structure of the kidney glomerulus capillary network

Abstract The capillary network of the kidney glomerulus filters small molecules from the blood. The glomerular 3D structure should help to understand its function, but it is poorly characterized. We therefore devised a new approach in which an automated tape collecting microtome (ATUM) was used to c...

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Autores principales:	Mark Terasaki, Jason Cory Brunson, Justin Sardi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5044044daccc49cd9fd1535923b3f667
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spelling	oai:doaj.org-article:5044044daccc49cd9fd1535923b3f6672021-12-02T16:08:37ZAnalysis of the three dimensional structure of the kidney glomerulus capillary network10.1038/s41598-020-77211-x2045-2322https://doaj.org/article/5044044daccc49cd9fd1535923b3f6672020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77211-xhttps://doaj.org/toc/2045-2322Abstract The capillary network of the kidney glomerulus filters small molecules from the blood. The glomerular 3D structure should help to understand its function, but it is poorly characterized. We therefore devised a new approach in which an automated tape collecting microtome (ATUM) was used to collect 0.5 μm thick serial sections from fixed mouse kidneys. The sections were imaged by scanning electron microscopy at ~ 50 nm/pixel resolution. With this approach, 12 glomeruli were reconstructed at an x–y–z resolution ~ 10 × higher than that of paraffin sections. We found a previously undescribed no-cross zone between afferent and efferent branches on the vascular pole side; connections here would allow blood to exit without being adequately filtered. The capillary diameters throughout the glomerulus appeared to correspond with the amount of blood flow within them. The shortest path (minimum number of branches to travel from afferent to efferent arterioles) is relatively independent of glomerular size and is present primarily on the vascular pole size. This suggests that new branches and longer paths form on the urinary pole side. Network analysis indicates that the glomerular network does not form by repetitive longitudinal splitting of capillaries. Thus the 3D structure of the glomerular capillary network provides useful information with which to understand glomerular function. Other tissue structures in the body may benefit from this new three dimensional approach.Mark TerasakiJason Cory BrunsonJustin SardiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mark Terasaki Jason Cory Brunson Justin Sardi Analysis of the three dimensional structure of the kidney glomerulus capillary network
description	Abstract The capillary network of the kidney glomerulus filters small molecules from the blood. The glomerular 3D structure should help to understand its function, but it is poorly characterized. We therefore devised a new approach in which an automated tape collecting microtome (ATUM) was used to collect 0.5 μm thick serial sections from fixed mouse kidneys. The sections were imaged by scanning electron microscopy at ~ 50 nm/pixel resolution. With this approach, 12 glomeruli were reconstructed at an x–y–z resolution ~ 10 × higher than that of paraffin sections. We found a previously undescribed no-cross zone between afferent and efferent branches on the vascular pole side; connections here would allow blood to exit without being adequately filtered. The capillary diameters throughout the glomerulus appeared to correspond with the amount of blood flow within them. The shortest path (minimum number of branches to travel from afferent to efferent arterioles) is relatively independent of glomerular size and is present primarily on the vascular pole size. This suggests that new branches and longer paths form on the urinary pole side. Network analysis indicates that the glomerular network does not form by repetitive longitudinal splitting of capillaries. Thus the 3D structure of the glomerular capillary network provides useful information with which to understand glomerular function. Other tissue structures in the body may benefit from this new three dimensional approach.
format	article
author	Mark Terasaki Jason Cory Brunson Justin Sardi
author_facet	Mark Terasaki Jason Cory Brunson Justin Sardi
author_sort	Mark Terasaki
title	Analysis of the three dimensional structure of the kidney glomerulus capillary network
title_short	Analysis of the three dimensional structure of the kidney glomerulus capillary network
title_full	Analysis of the three dimensional structure of the kidney glomerulus capillary network
title_fullStr	Analysis of the three dimensional structure of the kidney glomerulus capillary network
title_full_unstemmed	Analysis of the three dimensional structure of the kidney glomerulus capillary network
title_sort	analysis of the three dimensional structure of the kidney glomerulus capillary network
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5044044daccc49cd9fd1535923b3f667
work_keys_str_mv	AT markterasaki analysisofthethreedimensionalstructureofthekidneyglomeruluscapillarynetwork AT jasoncorybrunson analysisofthethreedimensionalstructureofthekidneyglomeruluscapillarynetwork AT justinsardi analysisofthethreedimensionalstructureofthekidneyglomeruluscapillarynetwork
_version_	1718384466551898112

Analysis of the three dimensional structure of the kidney glomerulus capillary network

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