Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver

Inès Azzouz, Hamdi Trabelsi, Amel Hanini, Soumaya Ferchichi, Olfa Tebourbi, Mohsen Sakly, Hafedh AbdelmelekLaboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, TunisiaAbstract: The aim of the present study was to investigate the interaction of zinc c...

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Autores principales:	Azzouz I, Trabelsi H, Hanini A, Ferchichi S, Tebourbi O, Sakly M, Abdelmelek H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5058d65c74ff41f88262fd5a90954348
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spelling	oai:doaj.org-article:5058d65c74ff41f88262fd5a909543482021-12-02T05:10:00ZInteraction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver1178-2013https://doaj.org/article/5058d65c74ff41f88262fd5a909543482013-12-01T00:00:00Zhttp://www.dovepress.com/interaction-between-nanoparticles-generated-by-zinc-chloride-treatment-a15394https://doaj.org/toc/1178-2013 Inès Azzouz, Hamdi Trabelsi, Amel Hanini, Soumaya Ferchichi, Olfa Tebourbi, Mohsen Sakly, Hafedh AbdelmelekLaboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, TunisiaAbstract: The aim of the present study was to investigate the interaction of zinc chloride (3 mg/kg, intraperitoneally [ip]) in rat liver in terms of the biosynthesis of nanoparticles. Zinc treatment increased zinc content in rat liver. Analysis of fluorescence revealed the presence of red fluorescence in the liver following zinc treatment. Interestingly, the co-exposure to zinc (3 mg/kg, ip) and selenium (0.20 mg/L, per os [by mouth]) led to a higher intensity of red fluorescence compared to zinc-treated rats. In addition, X-ray diffraction measurements carried out on liver fractions of zinc-treated rats point to the biosynthesis of zinc sulfide and/or selenide nanocomplexes at nearly 51.60 nm in size. Moreover, co-exposure led to nanocomplexes of about 72.60 nm in size. The interaction of zinc with other mineral elements (S, Se) generates several nanocomplexes, such as ZnS and/or ZnSe. The nanocomplex ZnX could interact directly with enzyme activity or indirectly by the disruption of mineral elements' bioavailability in cells. Subacute zinc or selenium treatment decreased malondialdehyde levels, indicating a drop in lipid peroxidation. In addition, antioxidant enzyme assays showed that treatment with zinc or co-treatment with zinc and selenium increased the activities of glutathione peroxidase, catalase, and superoxide dismutase. Consequently, zinc complexation with sulfur and/or selenium at nanoscale level could enhance antioxidative responses, which is correlated to the ratio of number of ZnX nanoparticles (X=sulfur or X=selenium) to malondialdehyde level in rat liver.Keywords: nanocomplexes biosynthesis, antioxidative responses, X-ray diffraction, fluorescence microscopy, liverAzzouz ITrabelsi HHanini AFerchichi STebourbi OSakly MAbdelmelek HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 223-229 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Azzouz I Trabelsi H Hanini A Ferchichi S Tebourbi O Sakly M Abdelmelek H Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver
description	Inès Azzouz, Hamdi Trabelsi, Amel Hanini, Soumaya Ferchichi, Olfa Tebourbi, Mohsen Sakly, Hafedh AbdelmelekLaboratory of Integrative Physiology, Faculty of Sciences of Bizerte, Carthage University, TunisiaAbstract: The aim of the present study was to investigate the interaction of zinc chloride (3 mg/kg, intraperitoneally [ip]) in rat liver in terms of the biosynthesis of nanoparticles. Zinc treatment increased zinc content in rat liver. Analysis of fluorescence revealed the presence of red fluorescence in the liver following zinc treatment. Interestingly, the co-exposure to zinc (3 mg/kg, ip) and selenium (0.20 mg/L, per os [by mouth]) led to a higher intensity of red fluorescence compared to zinc-treated rats. In addition, X-ray diffraction measurements carried out on liver fractions of zinc-treated rats point to the biosynthesis of zinc sulfide and/or selenide nanocomplexes at nearly 51.60 nm in size. Moreover, co-exposure led to nanocomplexes of about 72.60 nm in size. The interaction of zinc with other mineral elements (S, Se) generates several nanocomplexes, such as ZnS and/or ZnSe. The nanocomplex ZnX could interact directly with enzyme activity or indirectly by the disruption of mineral elements' bioavailability in cells. Subacute zinc or selenium treatment decreased malondialdehyde levels, indicating a drop in lipid peroxidation. In addition, antioxidant enzyme assays showed that treatment with zinc or co-treatment with zinc and selenium increased the activities of glutathione peroxidase, catalase, and superoxide dismutase. Consequently, zinc complexation with sulfur and/or selenium at nanoscale level could enhance antioxidative responses, which is correlated to the ratio of number of ZnX nanoparticles (X=sulfur or X=selenium) to malondialdehyde level in rat liver.Keywords: nanocomplexes biosynthesis, antioxidative responses, X-ray diffraction, fluorescence microscopy, liver
format	article
author	Azzouz I Trabelsi H Hanini A Ferchichi S Tebourbi O Sakly M Abdelmelek H
author_facet	Azzouz I Trabelsi H Hanini A Ferchichi S Tebourbi O Sakly M Abdelmelek H
author_sort	Azzouz I
title	Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver
title_short	Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver
title_full	Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver
title_fullStr	Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver
title_full_unstemmed	Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver
title_sort	interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/5058d65c74ff41f88262fd5a90954348
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Interaction between nanoparticles generated by zinc chloride treatment and oxidative responses in rat liver

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