Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.

Water-deficit stress is a major environmental factor that limits agricultural productivity worldwide. Recent episodes of extreme drought have severely affected cotton production in the Southwestern USA. There is a pressing need to develop cotton varieties with improved tolerance to water-deficit str...

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Autores principales:	Sundaram Kuppu, Neelam Mishra, Rongbin Hu, Li Sun, Xunlu Zhu, Guoxin Shen, Eduardo Blumwald, Paxton Payton, Hong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5061eaaa95b34978990b2529e7da734c
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spelling	oai:doaj.org-article:5061eaaa95b34978990b2529e7da734c2021-11-18T07:46:04ZWater-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.1932-620310.1371/journal.pone.0064190https://doaj.org/article/5061eaaa95b34978990b2529e7da734c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23675526/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Water-deficit stress is a major environmental factor that limits agricultural productivity worldwide. Recent episodes of extreme drought have severely affected cotton production in the Southwestern USA. There is a pressing need to develop cotton varieties with improved tolerance to water-deficit stress for sustainable production in water-limited regions. One approach to engineer drought tolerance is by delaying drought-induced senescence via up-regulation of cytokinin biosynthesis. The isopentenyltransferase gene (IPT) that encodes a rate limiting enzyme in cytokinin biosynthesis, under the control of a water-deficit responsive and maturation specific promoter P(SARK) was introduced into cotton and the performance of the P(SARK)::IPT transgenic cotton plants was analyzed in the greenhouse and growth chamber conditions. The data indicate that P(SARK)::IPT-transgenic cotton plants displayed delayed senescence under water deficit conditions in the greenhouse. These plants produced more root and shoot biomass, dropped fewer flowers, maintained higher chlorophyll content, and higher photosynthetic rates under reduced irrigation conditions in comparison to wild-type and segregated non-transgenic lines. Furthermore, P(SARK)::IPT-transgenic cotton plants grown in growth chamber condition also displayed greater drought tolerance. These results indicate that water-deficit induced expression of an isopentenyltransferase gene in cotton could significantly improve drought tolerance.Sundaram KuppuNeelam MishraRongbin HuLi SunXunlu ZhuGuoxin ShenEduardo BlumwaldPaxton PaytonHong ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64190 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sundaram Kuppu Neelam Mishra Rongbin Hu Li Sun Xunlu Zhu Guoxin Shen Eduardo Blumwald Paxton Payton Hong Zhang Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.
description	Water-deficit stress is a major environmental factor that limits agricultural productivity worldwide. Recent episodes of extreme drought have severely affected cotton production in the Southwestern USA. There is a pressing need to develop cotton varieties with improved tolerance to water-deficit stress for sustainable production in water-limited regions. One approach to engineer drought tolerance is by delaying drought-induced senescence via up-regulation of cytokinin biosynthesis. The isopentenyltransferase gene (IPT) that encodes a rate limiting enzyme in cytokinin biosynthesis, under the control of a water-deficit responsive and maturation specific promoter P(SARK) was introduced into cotton and the performance of the P(SARK)::IPT transgenic cotton plants was analyzed in the greenhouse and growth chamber conditions. The data indicate that P(SARK)::IPT-transgenic cotton plants displayed delayed senescence under water deficit conditions in the greenhouse. These plants produced more root and shoot biomass, dropped fewer flowers, maintained higher chlorophyll content, and higher photosynthetic rates under reduced irrigation conditions in comparison to wild-type and segregated non-transgenic lines. Furthermore, P(SARK)::IPT-transgenic cotton plants grown in growth chamber condition also displayed greater drought tolerance. These results indicate that water-deficit induced expression of an isopentenyltransferase gene in cotton could significantly improve drought tolerance.
format	article
author	Sundaram Kuppu Neelam Mishra Rongbin Hu Li Sun Xunlu Zhu Guoxin Shen Eduardo Blumwald Paxton Payton Hong Zhang
author_facet	Sundaram Kuppu Neelam Mishra Rongbin Hu Li Sun Xunlu Zhu Guoxin Shen Eduardo Blumwald Paxton Payton Hong Zhang
author_sort	Sundaram Kuppu
title	Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.
title_short	Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.
title_full	Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.
title_fullStr	Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.
title_full_unstemmed	Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.
title_sort	water-deficit inducible expression of a cytokinin biosynthetic gene ipt improves drought tolerance in cotton.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5061eaaa95b34978990b2529e7da734c
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Water-deficit inducible expression of a cytokinin biosynthetic gene IPT improves drought tolerance in cotton.

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