β-Galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer

Abstract Diagnosis of peritoneal metastasis in gastric cancer (GC) is essential for determining appropriate therapeutic strategies and avoiding non-essential laparotomy or gastrectomy. Recently, a variety of activatable fluorescence probes that can detect enzyme activities have been developed for ca...

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Autores principales:	Hidemasa Kubo, Yasutoshi Murayama, Soichiro Ogawa, Tatsuya Matsumoto, Masayuki Yubakami, Takuma Ohashi, Takeshi Kubota, Kazuma Okamoto, Mako Kamiya, Yasuteru Urano, Eigo Otsuji
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5062c0986b864e48831a9f71a4f29300
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spelling	oai:doaj.org-article:5062c0986b864e48831a9f71a4f293002021-12-02T15:45:32Zβ-Galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer10.1038/s41598-021-88982-22045-2322https://doaj.org/article/5062c0986b864e48831a9f71a4f293002021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88982-2https://doaj.org/toc/2045-2322Abstract Diagnosis of peritoneal metastasis in gastric cancer (GC) is essential for determining appropriate therapeutic strategies and avoiding non-essential laparotomy or gastrectomy. Recently, a variety of activatable fluorescence probes that can detect enzyme activities have been developed for cancer imaging. The aim of this study was to identify the key enzyme involved in peritoneal metastasis in GC. The enzymatic activity of gamma-glutamyl transpeptidase, dipeptidyl peptidase IV, and β-galactosidase (β-Gal) was assessed in lysates prepared from preserved human GC (n = 89) and normal peritoneal (NP; n = 20) samples. β-Gal activity was significantly higher in the human GC samples than in NP samples, whereas no differences were observed in the activities of the other enzymes. Therefore, we used SPiDER-βGal, a fluorescent probe that can be activated by β-Gal, for imaging GC cell lines, peritoneal metastasis in a mouse model, and fresh human resected GC samples (n = 13). All cell lines showed fluorescence after applying SPiDER-βGal, and metastatic nodules in the mice gradually developed high fluorescence that could be visualized with SPiDER-βGal. The human GC samples showed significantly higher fluorescence than NP samples. β-Gal is a useful target enzyme for fluorescence imaging of peritoneal metastasis in GC.Hidemasa KuboYasutoshi MurayamaSoichiro OgawaTatsuya MatsumotoMasayuki YubakamiTakuma OhashiTakeshi KubotaKazuma OkamotoMako KamiyaYasuteru UranoEigo OtsujiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hidemasa Kubo Yasutoshi Murayama Soichiro Ogawa Tatsuya Matsumoto Masayuki Yubakami Takuma Ohashi Takeshi Kubota Kazuma Okamoto Mako Kamiya Yasuteru Urano Eigo Otsuji β-Galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer
description	Abstract Diagnosis of peritoneal metastasis in gastric cancer (GC) is essential for determining appropriate therapeutic strategies and avoiding non-essential laparotomy or gastrectomy. Recently, a variety of activatable fluorescence probes that can detect enzyme activities have been developed for cancer imaging. The aim of this study was to identify the key enzyme involved in peritoneal metastasis in GC. The enzymatic activity of gamma-glutamyl transpeptidase, dipeptidyl peptidase IV, and β-galactosidase (β-Gal) was assessed in lysates prepared from preserved human GC (n = 89) and normal peritoneal (NP; n = 20) samples. β-Gal activity was significantly higher in the human GC samples than in NP samples, whereas no differences were observed in the activities of the other enzymes. Therefore, we used SPiDER-βGal, a fluorescent probe that can be activated by β-Gal, for imaging GC cell lines, peritoneal metastasis in a mouse model, and fresh human resected GC samples (n = 13). All cell lines showed fluorescence after applying SPiDER-βGal, and metastatic nodules in the mice gradually developed high fluorescence that could be visualized with SPiDER-βGal. The human GC samples showed significantly higher fluorescence than NP samples. β-Gal is a useful target enzyme for fluorescence imaging of peritoneal metastasis in GC.
format	article
author	Hidemasa Kubo Yasutoshi Murayama Soichiro Ogawa Tatsuya Matsumoto Masayuki Yubakami Takuma Ohashi Takeshi Kubota Kazuma Okamoto Mako Kamiya Yasuteru Urano Eigo Otsuji
author_facet	Hidemasa Kubo Yasutoshi Murayama Soichiro Ogawa Tatsuya Matsumoto Masayuki Yubakami Takuma Ohashi Takeshi Kubota Kazuma Okamoto Mako Kamiya Yasuteru Urano Eigo Otsuji
author_sort	Hidemasa Kubo
title	β-Galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer
title_short	β-Galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer
title_full	β-Galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer
title_fullStr	β-Galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer
title_full_unstemmed	β-Galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer
title_sort	β-galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5062c0986b864e48831a9f71a4f29300
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β-Galactosidase is a target enzyme for detecting peritoneal metastasis of gastric cancer

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