Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts

Thanavel Rajangam, Seong Soo A AnDepartment of Bionanotechnology, Gachon University, Seongnam, South KoreaAbstract: The aim of this study was to fabricate fibrinogen (Fbg) microfibers with different structural characteristics for the development of 3-D tissue-engineering scaffolds. Fabricated Fbg mi...

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Autores principales:	Rajangam T, A An SS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/50669ae914c64d65b09ab92e3fd40c76
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spelling	oai:doaj.org-article:50669ae914c64d65b09ab92e3fd40c762021-12-02T01:08:06ZImproved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts1176-91141178-2013https://doaj.org/article/50669ae914c64d65b09ab92e3fd40c762013-03-01T00:00:00Zhttp://www.dovepress.com/improved-fibronectin-immobilized-fibrinogen-microthreads-for-the-attac-a12443https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Thanavel Rajangam, Seong Soo A AnDepartment of Bionanotechnology, Gachon University, Seongnam, South KoreaAbstract: The aim of this study was to fabricate fibrinogen (Fbg) microfibers with different structural characteristics for the development of 3-D tissue-engineering scaffolds. Fabricated Fbg microfibers were investigated for their biomolecule encapsulation, cell adhesion, and proliferations. Microfibers with three different concentrations of Fbg (5, 10, and 15 wt%) were prepared by a gel solvent-extraction method using a silicone rubber tube. Fbg microfibers were covalently modified with fibronectin (FN) by using water-soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as the cross-linking agent. Fbg microfibers were characterized by their FN cross-linking properties, structural morphology, and in vitro degradation. Furthermore, FN/Fbg microfibers were evaluated for cell attachment and proliferation. The biocompatibility and cell proliferation of the microfibers were assessed by measuring adenosine triphosphate activity in C2C12 fibroblast cells. Cell attachment and proliferation on microfibers were further examined using fluorescence and scanning electron microscopic images. FN loading on the microfibers was confirmed by fluorescence and infrared spectroscopy. Surface morphology was characterized by scanning electron microscopy, and showed highly aligned nanostructures for fibers made with 15 wt% Fbg, a more porous structure for fibers made with 10 wt% Fbg, and a less porous structure for those made with 5 wt% Fbg. Controlled biodegradation of the fiber was observed for 8 weeks by using an in vitro proteolytic degradation assay. Fbg microfibers with highly aligned nanostructures (15 wt%) showed enhanced biomolecule encapsulation, as well as higher cell adhesion and proliferation than another two types of FN/Fbg fibers (5 and 10 wt%) and unmodified Fbg fibers. The promising results obtained from the present study reveal that optimal structure of Fbg microfibers could be used as a potential substratum for growth factors or drug release, especially in wound healing and vascular tissue engineering, in which fibers could be applied to promote and orient cell adhesion and proliferation.Keywords: fibrinogen, microfibers, nanofibers, cross-linking, fibronectin, tissue engineeringRajangam TA An SSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1037-1049 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Rajangam T A An SS Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts
description	Thanavel Rajangam, Seong Soo A AnDepartment of Bionanotechnology, Gachon University, Seongnam, South KoreaAbstract: The aim of this study was to fabricate fibrinogen (Fbg) microfibers with different structural characteristics for the development of 3-D tissue-engineering scaffolds. Fabricated Fbg microfibers were investigated for their biomolecule encapsulation, cell adhesion, and proliferations. Microfibers with three different concentrations of Fbg (5, 10, and 15 wt%) were prepared by a gel solvent-extraction method using a silicone rubber tube. Fbg microfibers were covalently modified with fibronectin (FN) by using water-soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as the cross-linking agent. Fbg microfibers were characterized by their FN cross-linking properties, structural morphology, and in vitro degradation. Furthermore, FN/Fbg microfibers were evaluated for cell attachment and proliferation. The biocompatibility and cell proliferation of the microfibers were assessed by measuring adenosine triphosphate activity in C2C12 fibroblast cells. Cell attachment and proliferation on microfibers were further examined using fluorescence and scanning electron microscopic images. FN loading on the microfibers was confirmed by fluorescence and infrared spectroscopy. Surface morphology was characterized by scanning electron microscopy, and showed highly aligned nanostructures for fibers made with 15 wt% Fbg, a more porous structure for fibers made with 10 wt% Fbg, and a less porous structure for those made with 5 wt% Fbg. Controlled biodegradation of the fiber was observed for 8 weeks by using an in vitro proteolytic degradation assay. Fbg microfibers with highly aligned nanostructures (15 wt%) showed enhanced biomolecule encapsulation, as well as higher cell adhesion and proliferation than another two types of FN/Fbg fibers (5 and 10 wt%) and unmodified Fbg fibers. The promising results obtained from the present study reveal that optimal structure of Fbg microfibers could be used as a potential substratum for growth factors or drug release, especially in wound healing and vascular tissue engineering, in which fibers could be applied to promote and orient cell adhesion and proliferation.Keywords: fibrinogen, microfibers, nanofibers, cross-linking, fibronectin, tissue engineering
format	article
author	Rajangam T A An SS
author_facet	Rajangam T A An SS
author_sort	Rajangam T
title	Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts
title_short	Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts
title_full	Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts
title_fullStr	Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts
title_full_unstemmed	Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts
title_sort	improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/50669ae914c64d65b09ab92e3fd40c76
work_keys_str_mv	AT rajangamt improvedfibronectinimmobilizedfibrinogenmicrothreadsfortheattachmentandproliferationoffibroblasts AT aanss improvedfibronectinimmobilizedfibrinogenmicrothreadsfortheattachmentandproliferationoffibroblasts
_version_	1718403253866070016

Improved fibronectin-immobilized fibrinogen microthreads for the attachment and proliferation of fibroblasts

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