Proteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium Anabaena PD-1 exposed to Aroclor 1254.

The cyanobacterium Anabaena PD-1, which was originally isolated from polychlorobiphenyl (PCB)-contaminated paddy soils, has capabilities for dechlorinatin and for degrading the commercial PCB mixture Aroclor 1254. In this study, 25 upregulated proteins were identified using 2D electrophoresis (2-DE)...

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Autores principales:	Hangjun Zhang, Xiaojun Jiang, Wenfeng Xiao, Liping Lu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/50744dc181bc4fcba68d18081a644666
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spelling	oai:doaj.org-article:50744dc181bc4fcba68d18081a6446662021-11-18T08:28:48ZProteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium Anabaena PD-1 exposed to Aroclor 1254.1932-620310.1371/journal.pone.0091162https://doaj.org/article/50744dc181bc4fcba68d18081a6446662014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24618583/?tool=EBIhttps://doaj.org/toc/1932-6203The cyanobacterium Anabaena PD-1, which was originally isolated from polychlorobiphenyl (PCB)-contaminated paddy soils, has capabilities for dechlorinatin and for degrading the commercial PCB mixture Aroclor 1254. In this study, 25 upregulated proteins were identified using 2D electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). These proteins were involved in (i) PCB degradation (i.e., 3-chlorobenzoate-3,4-dioxygenase); (ii) transport processes [e.g., ATP-binding cassette (ABC) transporter substrate-binding protein, amino acid ABC transporter substrate-binding protein, peptide ABC transporter substrate-binding protein, putrescine-binding protein, periplasmic solute-binding protein, branched-chain amino acid uptake periplasmic solute-binding protein, periplasmic phosphate-binding protein, phosphonate ABC transporter substrate-binding protein, and xylose ABC transporter substrate-binding protein]; (iii) energetic metabolism (e.g., methanol/ethanol family pyrroloquinoline quinone (PQQ)-dependent dehydrogenase, malate-CoA ligase subunit beta, enolase, ATP synthase β subunit, FOF1 ATP synthase subunit beta, ATP synthase α subunit, and IMP cyclohydrolase); (iv) electron transport (cytochrome b6f complex Fe-S protein); (v) general stress response (e.g., molecular chaperone DnaK, elongation factor G, and translation elongation factor thermostable); (vi) carbon metabolism (methanol dehydrogenase and malate-CoA ligase subunit beta); and (vii) nitrogen reductase (nitrous oxide reductase). The results of real-time polymerase chain reaction showed that the genes encoding for dioxygenase, ABC transporters, transmembrane proteins, electron transporter, and energetic metabolism proteins were significantly upregulated during PCB degradation. These genes upregulated by 1.26- to 8.98-fold. These findings reveal the resistance and adaptation of cyanobacterium to the presence of PCBs, shedding light on the complexity of PCB catabolism by Anabaena PD-1.Hangjun ZhangXiaojun JiangWenfeng XiaoLiping LuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91162 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hangjun Zhang Xiaojun Jiang Wenfeng Xiao Liping Lu Proteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium Anabaena PD-1 exposed to Aroclor 1254.
description	The cyanobacterium Anabaena PD-1, which was originally isolated from polychlorobiphenyl (PCB)-contaminated paddy soils, has capabilities for dechlorinatin and for degrading the commercial PCB mixture Aroclor 1254. In this study, 25 upregulated proteins were identified using 2D electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). These proteins were involved in (i) PCB degradation (i.e., 3-chlorobenzoate-3,4-dioxygenase); (ii) transport processes [e.g., ATP-binding cassette (ABC) transporter substrate-binding protein, amino acid ABC transporter substrate-binding protein, peptide ABC transporter substrate-binding protein, putrescine-binding protein, periplasmic solute-binding protein, branched-chain amino acid uptake periplasmic solute-binding protein, periplasmic phosphate-binding protein, phosphonate ABC transporter substrate-binding protein, and xylose ABC transporter substrate-binding protein]; (iii) energetic metabolism (e.g., methanol/ethanol family pyrroloquinoline quinone (PQQ)-dependent dehydrogenase, malate-CoA ligase subunit beta, enolase, ATP synthase β subunit, FOF1 ATP synthase subunit beta, ATP synthase α subunit, and IMP cyclohydrolase); (iv) electron transport (cytochrome b6f complex Fe-S protein); (v) general stress response (e.g., molecular chaperone DnaK, elongation factor G, and translation elongation factor thermostable); (vi) carbon metabolism (methanol dehydrogenase and malate-CoA ligase subunit beta); and (vii) nitrogen reductase (nitrous oxide reductase). The results of real-time polymerase chain reaction showed that the genes encoding for dioxygenase, ABC transporters, transmembrane proteins, electron transporter, and energetic metabolism proteins were significantly upregulated during PCB degradation. These genes upregulated by 1.26- to 8.98-fold. These findings reveal the resistance and adaptation of cyanobacterium to the presence of PCBs, shedding light on the complexity of PCB catabolism by Anabaena PD-1.
format	article
author	Hangjun Zhang Xiaojun Jiang Wenfeng Xiao Liping Lu
author_facet	Hangjun Zhang Xiaojun Jiang Wenfeng Xiao Liping Lu
author_sort	Hangjun Zhang
title	Proteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium Anabaena PD-1 exposed to Aroclor 1254.
title_short	Proteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium Anabaena PD-1 exposed to Aroclor 1254.
title_full	Proteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium Anabaena PD-1 exposed to Aroclor 1254.
title_fullStr	Proteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium Anabaena PD-1 exposed to Aroclor 1254.
title_full_unstemmed	Proteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium Anabaena PD-1 exposed to Aroclor 1254.
title_sort	proteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium anabaena pd-1 exposed to aroclor 1254.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/50744dc181bc4fcba68d18081a644666
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Proteomic strategy for the analysis of the polychlorobiphenyl-degrading cyanobacterium Anabaena PD-1 exposed to Aroclor 1254.

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