Deformability based Cell Sorting using Microfluidic Ratchets Enabling Phenotypic Separation of Leukocytes Directly from Whole Blood

Abstract The separation of leukocytes from whole blood is a prerequisite for many biological assays. Traditional methods require significant sample volumes and are often undesirable because they expose leukocytes to harsh physical or chemical treatment. Existing microfluidic approaches can work with...

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Autores principales:	Quan Guo, Simon P. Duffy, Kerryn Matthews, Emel Islamzada, Hongshen Ma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5074f8f2f4a3494a9d7cd6b70d5341fa
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spelling	oai:doaj.org-article:5074f8f2f4a3494a9d7cd6b70d5341fa2021-12-02T16:08:21ZDeformability based Cell Sorting using Microfluidic Ratchets Enabling Phenotypic Separation of Leukocytes Directly from Whole Blood10.1038/s41598-017-06865-x2045-2322https://doaj.org/article/5074f8f2f4a3494a9d7cd6b70d5341fa2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06865-xhttps://doaj.org/toc/2045-2322Abstract The separation of leukocytes from whole blood is a prerequisite for many biological assays. Traditional methods require significant sample volumes and are often undesirable because they expose leukocytes to harsh physical or chemical treatment. Existing microfluidic approaches can work with smaller volumes, but lack selectivity. In particular, the selectivity of microfluidic systems based on microfiltration is limited by fouling due to clogging. Here, we developed a method to separate leukocytes from whole blood using the microfluidic ratchet mechanism, which filters the blood sample using a matrix of micrometer-scale tapered constrictions. Deforming single cells through such constrictions requires directionally asymmetrical forces, which enables oscillatory flow to create a ratcheting transport that depends on cell size and deformability. Simultaneously, oscillatory flow continuously agitates the cells to limit the contact time with the filter microstructure to prevent adsorption and clogging. We show this device is capable of isolating leukocytes from whole blood with 100% purity (i.e. no contaminant erythrocytes) and <2% leukocytes loss. We further demonstrate the potential to phenotypically sort leukocytes to enrich for granulocytes and lymphocytes subpopulations. Together, this process provides a sensitive method to isolate and sort leukocytes directly from whole blood based on their biophysical properties.Quan GuoSimon P. DuffyKerryn MatthewsEmel IslamzadaHongshen MaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Quan Guo Simon P. Duffy Kerryn Matthews Emel Islamzada Hongshen Ma Deformability based Cell Sorting using Microfluidic Ratchets Enabling Phenotypic Separation of Leukocytes Directly from Whole Blood
description	Abstract The separation of leukocytes from whole blood is a prerequisite for many biological assays. Traditional methods require significant sample volumes and are often undesirable because they expose leukocytes to harsh physical or chemical treatment. Existing microfluidic approaches can work with smaller volumes, but lack selectivity. In particular, the selectivity of microfluidic systems based on microfiltration is limited by fouling due to clogging. Here, we developed a method to separate leukocytes from whole blood using the microfluidic ratchet mechanism, which filters the blood sample using a matrix of micrometer-scale tapered constrictions. Deforming single cells through such constrictions requires directionally asymmetrical forces, which enables oscillatory flow to create a ratcheting transport that depends on cell size and deformability. Simultaneously, oscillatory flow continuously agitates the cells to limit the contact time with the filter microstructure to prevent adsorption and clogging. We show this device is capable of isolating leukocytes from whole blood with 100% purity (i.e. no contaminant erythrocytes) and <2% leukocytes loss. We further demonstrate the potential to phenotypically sort leukocytes to enrich for granulocytes and lymphocytes subpopulations. Together, this process provides a sensitive method to isolate and sort leukocytes directly from whole blood based on their biophysical properties.
format	article
author	Quan Guo Simon P. Duffy Kerryn Matthews Emel Islamzada Hongshen Ma
author_facet	Quan Guo Simon P. Duffy Kerryn Matthews Emel Islamzada Hongshen Ma
author_sort	Quan Guo
title	Deformability based Cell Sorting using Microfluidic Ratchets Enabling Phenotypic Separation of Leukocytes Directly from Whole Blood
title_short	Deformability based Cell Sorting using Microfluidic Ratchets Enabling Phenotypic Separation of Leukocytes Directly from Whole Blood
title_full	Deformability based Cell Sorting using Microfluidic Ratchets Enabling Phenotypic Separation of Leukocytes Directly from Whole Blood
title_fullStr	Deformability based Cell Sorting using Microfluidic Ratchets Enabling Phenotypic Separation of Leukocytes Directly from Whole Blood
title_full_unstemmed	Deformability based Cell Sorting using Microfluidic Ratchets Enabling Phenotypic Separation of Leukocytes Directly from Whole Blood
title_sort	deformability based cell sorting using microfluidic ratchets enabling phenotypic separation of leukocytes directly from whole blood
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5074f8f2f4a3494a9d7cd6b70d5341fa
work_keys_str_mv	AT quanguo deformabilitybasedcellsortingusingmicrofluidicratchetsenablingphenotypicseparationofleukocytesdirectlyfromwholeblood AT simonpduffy deformabilitybasedcellsortingusingmicrofluidicratchetsenablingphenotypicseparationofleukocytesdirectlyfromwholeblood AT kerrynmatthews deformabilitybasedcellsortingusingmicrofluidicratchetsenablingphenotypicseparationofleukocytesdirectlyfromwholeblood AT emelislamzada deformabilitybasedcellsortingusingmicrofluidicratchetsenablingphenotypicseparationofleukocytesdirectlyfromwholeblood AT hongshenma deformabilitybasedcellsortingusingmicrofluidicratchetsenablingphenotypicseparationofleukocytesdirectlyfromwholeblood
_version_	1718384527978528768

Deformability based Cell Sorting using Microfluidic Ratchets Enabling Phenotypic Separation of Leukocytes Directly from Whole Blood

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