Subgingival Microbiota and Cytokines Profile Changes in Patients with Periodontitis: A Pilot Study Comparing Healthy and Diseased Sites in the Same Oral Cavities

Periodontitis is a common condition characterized by an exacerbated pro-inflammatory response, which leads to tissue destruction and, ultimately, alveolar bone loss. In this pilot study, we assess the microbiota composition and cytokine profile changes in patients with stage III/IV, grade B/C period...

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Autores principales:	Pauline Esparbès, Arnaud Legrand, Octave Nadile Bandiaky, Marjorie Chéraud-Carpentier, Hamida Martin, Emmanuel Montassier, Assem Soueidan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	subgingival microbiota periodontitis dysbiosis gingival crevicular fluid 16S rRNA gene sequencing Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/50789a6af13c4f4fb6f0b7af5878bc1f
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spelling	oai:doaj.org-article:50789a6af13c4f4fb6f0b7af5878bc1f2021-11-25T18:25:26ZSubgingival Microbiota and Cytokines Profile Changes in Patients with Periodontitis: A Pilot Study Comparing Healthy and Diseased Sites in the Same Oral Cavities10.3390/microorganisms91123642076-2607https://doaj.org/article/50789a6af13c4f4fb6f0b7af5878bc1f2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2364https://doaj.org/toc/2076-2607Periodontitis is a common condition characterized by an exacerbated pro-inflammatory response, which leads to tissue destruction and, ultimately, alveolar bone loss. In this pilot study, we assess the microbiota composition and cytokine profile changes in patients with stage III/IV, grade B/C periodontitis, specifically by comparing healthy and diseased sites in the same oral cavity. Overall, we found that microbiota architecture was significantly disrupted between diseased and healthy sites, and that the clustering was driven, in part, by the increased relative abundances of Synergistetes in diseased sites, as well as the increased abundances of Firmicutes in healthy sites. We also observed that diseased sites were enriched in Synergistetes, TM7, SR1, Spirochaetes, Bacteroidetes and Fusobacteria, and depleted in Firmicutes, Proteobacteria, Tenericutes and Actinobacteria compared to healthy sites. We found that Interleukin-1b, Interleukin-4, Interleukin-10, and Interleukin-17A were significantly overexpressed in diseased sites, whereas Interleukin-6 and TNF-alpha do not differ significantly between healthy and diseased sites. Here, we observed concomitant changes in the subgingival plaque microbiota and cytokines profile, suggesting that this combined alteration could contribute to the pathobiology of periodontitis.Pauline EsparbèsArnaud LegrandOctave Nadile BandiakyMarjorie Chéraud-CarpentierHamida MartinEmmanuel MontassierAssem SoueidanMDPI AGarticlesubgingival microbiotaperiodontitisdysbiosisgingival crevicular fluid16S rRNA gene sequencingBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2364, p 2364 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	subgingival microbiota periodontitis dysbiosis gingival crevicular fluid 16S rRNA gene sequencing Biology (General) QH301-705.5
spellingShingle	subgingival microbiota periodontitis dysbiosis gingival crevicular fluid 16S rRNA gene sequencing Biology (General) QH301-705.5 Pauline Esparbès Arnaud Legrand Octave Nadile Bandiaky Marjorie Chéraud-Carpentier Hamida Martin Emmanuel Montassier Assem Soueidan Subgingival Microbiota and Cytokines Profile Changes in Patients with Periodontitis: A Pilot Study Comparing Healthy and Diseased Sites in the Same Oral Cavities
description	Periodontitis is a common condition characterized by an exacerbated pro-inflammatory response, which leads to tissue destruction and, ultimately, alveolar bone loss. In this pilot study, we assess the microbiota composition and cytokine profile changes in patients with stage III/IV, grade B/C periodontitis, specifically by comparing healthy and diseased sites in the same oral cavity. Overall, we found that microbiota architecture was significantly disrupted between diseased and healthy sites, and that the clustering was driven, in part, by the increased relative abundances of Synergistetes in diseased sites, as well as the increased abundances of Firmicutes in healthy sites. We also observed that diseased sites were enriched in Synergistetes, TM7, SR1, Spirochaetes, Bacteroidetes and Fusobacteria, and depleted in Firmicutes, Proteobacteria, Tenericutes and Actinobacteria compared to healthy sites. We found that Interleukin-1b, Interleukin-4, Interleukin-10, and Interleukin-17A were significantly overexpressed in diseased sites, whereas Interleukin-6 and TNF-alpha do not differ significantly between healthy and diseased sites. Here, we observed concomitant changes in the subgingival plaque microbiota and cytokines profile, suggesting that this combined alteration could contribute to the pathobiology of periodontitis.
format	article
author	Pauline Esparbès Arnaud Legrand Octave Nadile Bandiaky Marjorie Chéraud-Carpentier Hamida Martin Emmanuel Montassier Assem Soueidan
author_facet	Pauline Esparbès Arnaud Legrand Octave Nadile Bandiaky Marjorie Chéraud-Carpentier Hamida Martin Emmanuel Montassier Assem Soueidan
author_sort	Pauline Esparbès
title	Subgingival Microbiota and Cytokines Profile Changes in Patients with Periodontitis: A Pilot Study Comparing Healthy and Diseased Sites in the Same Oral Cavities
title_short	Subgingival Microbiota and Cytokines Profile Changes in Patients with Periodontitis: A Pilot Study Comparing Healthy and Diseased Sites in the Same Oral Cavities
title_full	Subgingival Microbiota and Cytokines Profile Changes in Patients with Periodontitis: A Pilot Study Comparing Healthy and Diseased Sites in the Same Oral Cavities
title_fullStr	Subgingival Microbiota and Cytokines Profile Changes in Patients with Periodontitis: A Pilot Study Comparing Healthy and Diseased Sites in the Same Oral Cavities
title_full_unstemmed	Subgingival Microbiota and Cytokines Profile Changes in Patients with Periodontitis: A Pilot Study Comparing Healthy and Diseased Sites in the Same Oral Cavities
title_sort	subgingival microbiota and cytokines profile changes in patients with periodontitis: a pilot study comparing healthy and diseased sites in the same oral cavities
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/50789a6af13c4f4fb6f0b7af5878bc1f
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Subgingival Microbiota and Cytokines Profile Changes in Patients with Periodontitis: A Pilot Study Comparing Healthy and Diseased Sites in the Same Oral Cavities

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