Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions

Abstract Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are gaining increasing importance in the field of regenerative medicine. Although therapeutic value of MSCs is now being established through many clinical trials, issues have been raised regarding their expansion as per regulatory guid...

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Autores principales:	Samatha Bhat, Pachaiyappan Viswanathan, Shashank Chandanala, S. Jyothi Prasanna, Raviraja N. Seetharam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/507e5f051ff34c1ba30abd9f3c42903b
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spelling	oai:doaj.org-article:507e5f051ff34c1ba30abd9f3c42903b2021-12-02T14:26:48ZExpansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions10.1038/s41598-021-83088-12045-2322https://doaj.org/article/507e5f051ff34c1ba30abd9f3c42903b2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83088-1https://doaj.org/toc/2045-2322Abstract Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are gaining increasing importance in the field of regenerative medicine. Although therapeutic value of MSCs is now being established through many clinical trials, issues have been raised regarding their expansion as per regulatory guidelines. Fetal bovine serum usage in cell therapy poses difficulties due to its less-defined, highly variable composition and safety issues. Hence, there is a need for transition from serum-based to serum-free media (SFM). Since SFM are cell type-specific, a precise analysis of the properties of MSCs cultured in SFM is required to determine the most suitable one. Six different commercially available low serum/SFM with two different seeding densities were evaluated to explore their ability to support the growth and expansion of BM-MSCs and assess the characteristics of BM-MSCs cultured in these media. Except for one of the SFM, all other media tested supported the growth of BM-MSCs at a low seeding density. No significant differences were observed in the expression of MSC specific markers among the various media tested. In contrary, the population doubling time, cell yield, potency, colony-forming ability, differentiation potential, and immunosuppressive properties of MSCs varied with one another. We show that SFM tested supports the growth and expansion of BM-MSCs even at low seeding density and may serve as possible replacement for animal-derived serum.Samatha BhatPachaiyappan ViswanathanShashank ChandanalaS. Jyothi PrasannaRaviraja N. SeetharamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samatha Bhat Pachaiyappan Viswanathan Shashank Chandanala S. Jyothi Prasanna Raviraja N. Seetharam Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions
description	Abstract Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are gaining increasing importance in the field of regenerative medicine. Although therapeutic value of MSCs is now being established through many clinical trials, issues have been raised regarding their expansion as per regulatory guidelines. Fetal bovine serum usage in cell therapy poses difficulties due to its less-defined, highly variable composition and safety issues. Hence, there is a need for transition from serum-based to serum-free media (SFM). Since SFM are cell type-specific, a precise analysis of the properties of MSCs cultured in SFM is required to determine the most suitable one. Six different commercially available low serum/SFM with two different seeding densities were evaluated to explore their ability to support the growth and expansion of BM-MSCs and assess the characteristics of BM-MSCs cultured in these media. Except for one of the SFM, all other media tested supported the growth of BM-MSCs at a low seeding density. No significant differences were observed in the expression of MSC specific markers among the various media tested. In contrary, the population doubling time, cell yield, potency, colony-forming ability, differentiation potential, and immunosuppressive properties of MSCs varied with one another. We show that SFM tested supports the growth and expansion of BM-MSCs even at low seeding density and may serve as possible replacement for animal-derived serum.
format	article
author	Samatha Bhat Pachaiyappan Viswanathan Shashank Chandanala S. Jyothi Prasanna Raviraja N. Seetharam
author_facet	Samatha Bhat Pachaiyappan Viswanathan Shashank Chandanala S. Jyothi Prasanna Raviraja N. Seetharam
author_sort	Samatha Bhat
title	Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions
title_short	Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions
title_full	Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions
title_fullStr	Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions
title_full_unstemmed	Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions
title_sort	expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/507e5f051ff34c1ba30abd9f3c42903b
work_keys_str_mv	AT samathabhat expansionandcharacterizationofbonemarrowderivedhumanmesenchymalstromalcellsinserumfreeconditions AT pachaiyappanviswanathan expansionandcharacterizationofbonemarrowderivedhumanmesenchymalstromalcellsinserumfreeconditions AT shashankchandanala expansionandcharacterizationofbonemarrowderivedhumanmesenchymalstromalcellsinserumfreeconditions AT sjyothiprasanna expansionandcharacterizationofbonemarrowderivedhumanmesenchymalstromalcellsinserumfreeconditions AT ravirajanseetharam expansionandcharacterizationofbonemarrowderivedhumanmesenchymalstromalcellsinserumfreeconditions
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Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions

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