An organic NIR-II nanofluorophore with aggregation-induced emission characteristics for in vivo fluorescence imaging
Wei Wu,1,* Yan-Qing Yang,2,* Yang Yang,1,* Yu-Ming Yang,2 Hong Wang,2 Kai-Yuan Zhang,1 Li Guo,3 Hong-Fei Ge,1 Jie Liu,2 Hua Feng11Department of Neurosurgery, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, People’s Republic of China; 2Key...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2019
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/5080c55b28234fe29e46594690f61faa |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:5080c55b28234fe29e46594690f61faa |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:5080c55b28234fe29e46594690f61faa2021-12-02T08:14:19ZAn organic NIR-II nanofluorophore with aggregation-induced emission characteristics for in vivo fluorescence imaging1178-2013https://doaj.org/article/5080c55b28234fe29e46594690f61faa2019-05-01T00:00:00Zhttps://www.dovepress.com/an-organic-nir-ii-nanofluorophore-with-aggregation-induced-emission-ch-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wei Wu,1,* Yan-Qing Yang,2,* Yang Yang,1,* Yu-Ming Yang,2 Hong Wang,2 Kai-Yuan Zhang,1 Li Guo,3 Hong-Fei Ge,1 Jie Liu,2 Hua Feng11Department of Neurosurgery, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, People’s Republic of China; 2Key Laboratory of Flexible Electronics (KLOFE) Institute of Advanced Materials (IAM), Nanjing Tech University, Nanjing 211800, People’s Republic of China; 3Department of Endocrinology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, People’s Republic of China*These authors contributed equally to this workBackground: In vivo fluorescence imaging in the second near-infrared (NIR-II, 1000–1700 nm) window using organic fluorophores has great advantages, but generally suffers from a relatively low fluorescence quantum yield (mostly less than 2%). In this study, organic nanoparticles (L1013 NPs) with a high fluorescence quantum yield (9.9%) were systhesized for in vivo imaging.Methods: A molecule (BTPPA) with donor-acceptor-donor structure and aggregation-induced emission enabling moieties was prepared. BTPPA molecules were then encapsulated into nanoparticles (L1013 NPs) using a nanoprecipitation method. The L1013 NPs were intravenously injected into the mice (including normal, stroke and tumor models) for vascular and tumor imaging.Results: L1013 NPs excited at 808 nm exhibit NIR-II emission with a peak at 1013 nm and an emission tail extending to 1400 nm. They have a quantum yield of 9.9% and also show excellent photo/colloidal stabilities and negligible in vitro and in vivo toxicity. We use L1013 NPs for noninvasive real-time visualization of mouse hindlimb and cerebral vessels (including stroke pathology) under a very low power density (4.6–40 mW cm‒2) and short exposure time (40–100 ms). Moreover, L1013 NPs are able to localize tumor pathology, with a tumor-to-normal tissue ratio of 11.7±1.3, which is unusually high for NIR-II fluorescent imaging through passive targeting strategy.Conclusion: L1013 NPs demonstrate the potential for a range of clinical applications, especially for tumor surgery.Keywords: fluorescence NIR-II imaging, aggregation-induced emission, organic nanoparticle, vascular imaging, tumor imagingWu WYang YQYang YYang YMWang HZhang KYGuo LGe HFLiu JFeng HDove Medical Pressarticlefluorescence NIR-II imagingaggregation-induced emissionorganic nanoparticlevascular imagingtumor imagingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3571-3582 (2019) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
fluorescence NIR-II imaging aggregation-induced emission organic nanoparticle vascular imaging tumor imaging Medicine (General) R5-920 |
| spellingShingle |
fluorescence NIR-II imaging aggregation-induced emission organic nanoparticle vascular imaging tumor imaging Medicine (General) R5-920 Wu W Yang YQ Yang Y Yang YM Wang H Zhang KY Guo L Ge HF Liu J Feng H An organic NIR-II nanofluorophore with aggregation-induced emission characteristics for in vivo fluorescence imaging |
| description |
Wei Wu,1,* Yan-Qing Yang,2,* Yang Yang,1,* Yu-Ming Yang,2 Hong Wang,2 Kai-Yuan Zhang,1 Li Guo,3 Hong-Fei Ge,1 Jie Liu,2 Hua Feng11Department of Neurosurgery, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, People’s Republic of China; 2Key Laboratory of Flexible Electronics (KLOFE) Institute of Advanced Materials (IAM), Nanjing Tech University, Nanjing 211800, People’s Republic of China; 3Department of Endocrinology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, People’s Republic of China*These authors contributed equally to this workBackground: In vivo fluorescence imaging in the second near-infrared (NIR-II, 1000–1700 nm) window using organic fluorophores has great advantages, but generally suffers from a relatively low fluorescence quantum yield (mostly less than 2%). In this study, organic nanoparticles (L1013 NPs) with a high fluorescence quantum yield (9.9%) were systhesized for in vivo imaging.Methods: A molecule (BTPPA) with donor-acceptor-donor structure and aggregation-induced emission enabling moieties was prepared. BTPPA molecules were then encapsulated into nanoparticles (L1013 NPs) using a nanoprecipitation method. The L1013 NPs were intravenously injected into the mice (including normal, stroke and tumor models) for vascular and tumor imaging.Results: L1013 NPs excited at 808 nm exhibit NIR-II emission with a peak at 1013 nm and an emission tail extending to 1400 nm. They have a quantum yield of 9.9% and also show excellent photo/colloidal stabilities and negligible in vitro and in vivo toxicity. We use L1013 NPs for noninvasive real-time visualization of mouse hindlimb and cerebral vessels (including stroke pathology) under a very low power density (4.6–40 mW cm‒2) and short exposure time (40–100 ms). Moreover, L1013 NPs are able to localize tumor pathology, with a tumor-to-normal tissue ratio of 11.7±1.3, which is unusually high for NIR-II fluorescent imaging through passive targeting strategy.Conclusion: L1013 NPs demonstrate the potential for a range of clinical applications, especially for tumor surgery.Keywords: fluorescence NIR-II imaging, aggregation-induced emission, organic nanoparticle, vascular imaging, tumor imaging |
| format |
article |
| author |
Wu W Yang YQ Yang Y Yang YM Wang H Zhang KY Guo L Ge HF Liu J Feng H |
| author_facet |
Wu W Yang YQ Yang Y Yang YM Wang H Zhang KY Guo L Ge HF Liu J Feng H |
| author_sort |
Wu W |
| title |
An organic NIR-II nanofluorophore with aggregation-induced emission characteristics for in vivo fluorescence imaging |
| title_short |
An organic NIR-II nanofluorophore with aggregation-induced emission characteristics for in vivo fluorescence imaging |
| title_full |
An organic NIR-II nanofluorophore with aggregation-induced emission characteristics for in vivo fluorescence imaging |
| title_fullStr |
An organic NIR-II nanofluorophore with aggregation-induced emission characteristics for in vivo fluorescence imaging |
| title_full_unstemmed |
An organic NIR-II nanofluorophore with aggregation-induced emission characteristics for in vivo fluorescence imaging |
| title_sort |
organic nir-ii nanofluorophore with aggregation-induced emission characteristics for in vivo fluorescence imaging |
| publisher |
Dove Medical Press |
| publishDate |
2019 |
| url |
https://doaj.org/article/5080c55b28234fe29e46594690f61faa |
| work_keys_str_mv |
AT wuw anorganicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT yangyq anorganicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT yangy anorganicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT yangym anorganicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT wangh anorganicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT zhangky anorganicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT guol anorganicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT gehf anorganicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT liuj anorganicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT fengh anorganicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT wuw organicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT yangyq organicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT yangy organicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT yangym organicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT wangh organicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT zhangky organicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT guol organicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT gehf organicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT liuj organicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging AT fengh organicniriinanofluorophorewithaggregationinducedemissioncharacteristicsforinvivofluorescenceimaging |
| _version_ |
1718398599042170880 |