High-Throughput, Label-Free Isolation of White Blood Cells from Whole Blood Using Parallel Spiral Microchannels with U-Shaped Cross-Section

Rapid isolation of white blood cells (WBCs) from whole blood is an essential part of any WBC examination platform. However, most conventional cell separation techniques are labor-intensive and low throughput, require large volumes of samples, need extensive cell manipulation, and have low purity. To...

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Autores principales:	Amirhossein Mehran, Peyman Rostami, Mohammad Said Saidi, Bahar Firoozabadi, Navid Kashaninejad
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	WBC isolation spiral microchannels inertial microfluidics passive cell separation high-throughput separation Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/5081587b52fe4e3f8a63d86d5c4880f7
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spelling	oai:doaj.org-article:5081587b52fe4e3f8a63d86d5c4880f72021-11-25T16:54:48ZHigh-Throughput, Label-Free Isolation of White Blood Cells from Whole Blood Using Parallel Spiral Microchannels with U-Shaped Cross-Section10.3390/bios111104062079-6374https://doaj.org/article/5081587b52fe4e3f8a63d86d5c4880f72021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/406https://doaj.org/toc/2079-6374Rapid isolation of white blood cells (WBCs) from whole blood is an essential part of any WBC examination platform. However, most conventional cell separation techniques are labor-intensive and low throughput, require large volumes of samples, need extensive cell manipulation, and have low purity. To address these challenges, we report the design and fabrication of a passive, label-free microfluidic device with a unique U-shaped cross-section to separate WBCs from whole blood using hydrodynamic forces that exist in a microchannel with curvilinear geometry. It is shown that the spiral microchannel with a U-shaped cross-section concentrates larger blood cells (e.g., WBCs) in the inner cross-section of the microchannel by moving smaller blood cells (e.g., RBCs and platelets) to the outer microchannel section and preventing them from returning to the inner microchannel section. Therefore, it overcomes the major limitation of a rectangular cross-section where secondary Dean vortices constantly enforce particles throughout the entire cross-section and decrease its isolation efficiency. Under optimal settings, we managed to isolate more than 95% of WBCs from whole blood under high-throughput (6 mL/min), high-purity (88%), and high-capacity (360 mL of sample in 1 h) conditions. High efficiency, fast processing time, and non-invasive WBC isolation from large blood samples without centrifugation, RBC lysis, cell biomarkers, and chemical pre-treatments make this method an ideal choice for downstream cell study platforms.Amirhossein MehranPeyman RostamiMohammad Said SaidiBahar FiroozabadiNavid KashaninejadMDPI AGarticleWBC isolationspiral microchannelsinertial microfluidicspassive cell separationhigh-throughput separationBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 406, p 406 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	WBC isolation spiral microchannels inertial microfluidics passive cell separation high-throughput separation Biotechnology TP248.13-248.65
spellingShingle	WBC isolation spiral microchannels inertial microfluidics passive cell separation high-throughput separation Biotechnology TP248.13-248.65 Amirhossein Mehran Peyman Rostami Mohammad Said Saidi Bahar Firoozabadi Navid Kashaninejad High-Throughput, Label-Free Isolation of White Blood Cells from Whole Blood Using Parallel Spiral Microchannels with U-Shaped Cross-Section
description	Rapid isolation of white blood cells (WBCs) from whole blood is an essential part of any WBC examination platform. However, most conventional cell separation techniques are labor-intensive and low throughput, require large volumes of samples, need extensive cell manipulation, and have low purity. To address these challenges, we report the design and fabrication of a passive, label-free microfluidic device with a unique U-shaped cross-section to separate WBCs from whole blood using hydrodynamic forces that exist in a microchannel with curvilinear geometry. It is shown that the spiral microchannel with a U-shaped cross-section concentrates larger blood cells (e.g., WBCs) in the inner cross-section of the microchannel by moving smaller blood cells (e.g., RBCs and platelets) to the outer microchannel section and preventing them from returning to the inner microchannel section. Therefore, it overcomes the major limitation of a rectangular cross-section where secondary Dean vortices constantly enforce particles throughout the entire cross-section and decrease its isolation efficiency. Under optimal settings, we managed to isolate more than 95% of WBCs from whole blood under high-throughput (6 mL/min), high-purity (88%), and high-capacity (360 mL of sample in 1 h) conditions. High efficiency, fast processing time, and non-invasive WBC isolation from large blood samples without centrifugation, RBC lysis, cell biomarkers, and chemical pre-treatments make this method an ideal choice for downstream cell study platforms.
format	article
author	Amirhossein Mehran Peyman Rostami Mohammad Said Saidi Bahar Firoozabadi Navid Kashaninejad
author_facet	Amirhossein Mehran Peyman Rostami Mohammad Said Saidi Bahar Firoozabadi Navid Kashaninejad
author_sort	Amirhossein Mehran
title	High-Throughput, Label-Free Isolation of White Blood Cells from Whole Blood Using Parallel Spiral Microchannels with U-Shaped Cross-Section
title_short	High-Throughput, Label-Free Isolation of White Blood Cells from Whole Blood Using Parallel Spiral Microchannels with U-Shaped Cross-Section
title_full	High-Throughput, Label-Free Isolation of White Blood Cells from Whole Blood Using Parallel Spiral Microchannels with U-Shaped Cross-Section
title_fullStr	High-Throughput, Label-Free Isolation of White Blood Cells from Whole Blood Using Parallel Spiral Microchannels with U-Shaped Cross-Section
title_full_unstemmed	High-Throughput, Label-Free Isolation of White Blood Cells from Whole Blood Using Parallel Spiral Microchannels with U-Shaped Cross-Section
title_sort	high-throughput, label-free isolation of white blood cells from whole blood using parallel spiral microchannels with u-shaped cross-section
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5081587b52fe4e3f8a63d86d5c4880f7
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High-Throughput, Label-Free Isolation of White Blood Cells from Whole Blood Using Parallel Spiral Microchannels with U-Shaped Cross-Section

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