Identification of lncRNA and mRNA Expression Profile in Relapsed Graves’ Disease

Background: Graves’ disease (GD) is a common autoimmune disease, and its pathogenesis is unclear. Studies have found that the occurrence of GD is related to the immune disorder caused by the interaction of genetic susceptibility and environmental factors. The CD4+ T cell subset is closely related to...

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Autores principales:	Qiuming Yao, Zhenyu Song, Bin Wang, Xi Jia, Ronghua Song, Jinan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lncRNAs relapsed GD WGCNA NONHSAT093153.2 NONHSAT209004.1 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5086f6a98a524125ba534780068aaae2
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spelling	oai:doaj.org-article:5086f6a98a524125ba534780068aaae22021-12-02T01:34:40ZIdentification of lncRNA and mRNA Expression Profile in Relapsed Graves’ Disease2296-634X10.3389/fcell.2021.756560https://doaj.org/article/5086f6a98a524125ba534780068aaae22021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.756560/fullhttps://doaj.org/toc/2296-634XBackground: Graves’ disease (GD) is a common autoimmune disease, and its pathogenesis is unclear. Studies have found that the occurrence of GD is related to the immune disorder caused by the interaction of genetic susceptibility and environmental factors. The CD4+ T cell subset is closely related to the immune disorder of GD. LncRNAs are RNA molecules with a length of more than 200 nt and are involved in a variety of autoimmune diseases. However, the roles of lncRNAs in recurrent GD are still elusive. The purpose of this study is to identify lncRNA and mRNA expression profile in relapsed Graves’ disease.Method: CD4+ T cells from 12 recurrent GD and 8 healthy controls were collected for high-throughput sequencing. The gene-weighted co-expression network analysis (WGCNA) was used to construct the co-expression module relevant to recurrent GD, and the key genes in the module were verified by RT-PCR.Results: There are 602 upregulated lncRNAs and 734 downregulated lncRNAs in CD4+ T cells in recurrent GD patients compared with the healthy controls. The module most relevant to GD recurrence was constructed using WGCNA, and the key genes in the module were verified by RT-PCR. We found that the expression of RPL8, OAS2, NFAT5, DROSHA, NONHSAT093153.2, NONHSAT118924.2, and NONHSAT209004.1 was significantly decreased in GD group (p < 0.001, p < 0.001, p < 0.01, p < 0.05, p < 0.001, p < 0.05, and p < 0.01, respectively).Conclusion: LncRNAs are closely related to the recurrence of GD. For the first time, we constructed the expression profile of lncRNAs and mRNAs in CD4+ T cells in recurrent GD patients.Qiuming YaoZhenyu SongBin WangXi JiaRonghua SongJinan ZhangFrontiers Media S.A.articlelncRNAsrelapsed GDWGCNANONHSAT093153.2NONHSAT209004.1Biology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lncRNAs relapsed GD WGCNA NONHSAT093153.2 NONHSAT209004.1 Biology (General) QH301-705.5
spellingShingle	lncRNAs relapsed GD WGCNA NONHSAT093153.2 NONHSAT209004.1 Biology (General) QH301-705.5 Qiuming Yao Zhenyu Song Bin Wang Xi Jia Ronghua Song Jinan Zhang Identification of lncRNA and mRNA Expression Profile in Relapsed Graves’ Disease
description	Background: Graves’ disease (GD) is a common autoimmune disease, and its pathogenesis is unclear. Studies have found that the occurrence of GD is related to the immune disorder caused by the interaction of genetic susceptibility and environmental factors. The CD4+ T cell subset is closely related to the immune disorder of GD. LncRNAs are RNA molecules with a length of more than 200 nt and are involved in a variety of autoimmune diseases. However, the roles of lncRNAs in recurrent GD are still elusive. The purpose of this study is to identify lncRNA and mRNA expression profile in relapsed Graves’ disease.Method: CD4+ T cells from 12 recurrent GD and 8 healthy controls were collected for high-throughput sequencing. The gene-weighted co-expression network analysis (WGCNA) was used to construct the co-expression module relevant to recurrent GD, and the key genes in the module were verified by RT-PCR.Results: There are 602 upregulated lncRNAs and 734 downregulated lncRNAs in CD4+ T cells in recurrent GD patients compared with the healthy controls. The module most relevant to GD recurrence was constructed using WGCNA, and the key genes in the module were verified by RT-PCR. We found that the expression of RPL8, OAS2, NFAT5, DROSHA, NONHSAT093153.2, NONHSAT118924.2, and NONHSAT209004.1 was significantly decreased in GD group (p < 0.001, p < 0.001, p < 0.01, p < 0.05, p < 0.001, p < 0.05, and p < 0.01, respectively).Conclusion: LncRNAs are closely related to the recurrence of GD. For the first time, we constructed the expression profile of lncRNAs and mRNAs in CD4+ T cells in recurrent GD patients.
format	article
author	Qiuming Yao Zhenyu Song Bin Wang Xi Jia Ronghua Song Jinan Zhang
author_facet	Qiuming Yao Zhenyu Song Bin Wang Xi Jia Ronghua Song Jinan Zhang
author_sort	Qiuming Yao
title	Identification of lncRNA and mRNA Expression Profile in Relapsed Graves’ Disease
title_short	Identification of lncRNA and mRNA Expression Profile in Relapsed Graves’ Disease
title_full	Identification of lncRNA and mRNA Expression Profile in Relapsed Graves’ Disease
title_fullStr	Identification of lncRNA and mRNA Expression Profile in Relapsed Graves’ Disease
title_full_unstemmed	Identification of lncRNA and mRNA Expression Profile in Relapsed Graves’ Disease
title_sort	identification of lncrna and mrna expression profile in relapsed graves’ disease
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5086f6a98a524125ba534780068aaae2
work_keys_str_mv	AT qiumingyao identificationoflncrnaandmrnaexpressionprofileinrelapsedgravesdisease AT zhenyusong identificationoflncrnaandmrnaexpressionprofileinrelapsedgravesdisease AT binwang identificationoflncrnaandmrnaexpressionprofileinrelapsedgravesdisease AT xijia identificationoflncrnaandmrnaexpressionprofileinrelapsedgravesdisease AT ronghuasong identificationoflncrnaandmrnaexpressionprofileinrelapsedgravesdisease AT jinanzhang identificationoflncrnaandmrnaexpressionprofileinrelapsedgravesdisease
_version_	1718402956731088896

Identification of lncRNA and mRNA Expression Profile in Relapsed Graves’ Disease

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