Does the Evidence Support the Existence of the Simian Polyomavirus SV40 Vp4 Viroporin?

ABSTRACT The simian polyomavirus SV40 was reported to express Vp4, an N-terminally truncated form of the minor capsid proteins Vp2 and Vp3. Since a missense mutation of the putative Vp4 start codon (Vp2M228I) was found to give reduced progeny release and delayed lysis, Vp4 was claimed to be a viropo...

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Autores principales:	Stian Henriksen, Christine Hanssen Rinaldo
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	BK virus BKPyV SV40 Vp4 polyomavirus simian virus 40 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/508c88e75c524b7b90703fc4c783ce80
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spelling	oai:doaj.org-article:508c88e75c524b7b90703fc4c783ce802021-11-15T15:29:17ZDoes the Evidence Support the Existence of the Simian Polyomavirus SV40 Vp4 Viroporin?10.1128/mSphere.00019-202379-5042https://doaj.org/article/508c88e75c524b7b90703fc4c783ce802020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00019-20https://doaj.org/toc/2379-5042ABSTRACT The simian polyomavirus SV40 was reported to express Vp4, an N-terminally truncated form of the minor capsid proteins Vp2 and Vp3. Since a missense mutation of the putative Vp4 start codon (Vp2M228I) was found to give reduced progeny release and delayed lysis, Vp4 was claimed to be a viroporin. However, two independent research groups, including our own, were unable to replicate these findings. In contrast, we found no Vp4 expression in SV40-infected cells and no reduction in progeny release for Vp4-deficient virus, and finally, we found that the single amino acid substitution unavoidably introduced into the overlapping Vp2/Vp3 genes during Vp4 mutagenesis reduced early steps but not virus release. Remarkably, the existence of the viroporin Vp4 still seems to be widely accepted, which presumably is preventing important research on polyomavirus release. With this perspective, we will review and comment on the most important experiments that led to the disputed announcement of the viroporin Vp4.Stian HenriksenChristine Hanssen RinaldoAmerican Society for MicrobiologyarticleBK virusBKPyVSV40Vp4polyomavirussimian virus 40MicrobiologyQR1-502ENmSphere, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	BK virus BKPyV SV40 Vp4 polyomavirus simian virus 40 Microbiology QR1-502
spellingShingle	BK virus BKPyV SV40 Vp4 polyomavirus simian virus 40 Microbiology QR1-502 Stian Henriksen Christine Hanssen Rinaldo Does the Evidence Support the Existence of the Simian Polyomavirus SV40 Vp4 Viroporin?
description	ABSTRACT The simian polyomavirus SV40 was reported to express Vp4, an N-terminally truncated form of the minor capsid proteins Vp2 and Vp3. Since a missense mutation of the putative Vp4 start codon (Vp2M228I) was found to give reduced progeny release and delayed lysis, Vp4 was claimed to be a viroporin. However, two independent research groups, including our own, were unable to replicate these findings. In contrast, we found no Vp4 expression in SV40-infected cells and no reduction in progeny release for Vp4-deficient virus, and finally, we found that the single amino acid substitution unavoidably introduced into the overlapping Vp2/Vp3 genes during Vp4 mutagenesis reduced early steps but not virus release. Remarkably, the existence of the viroporin Vp4 still seems to be widely accepted, which presumably is preventing important research on polyomavirus release. With this perspective, we will review and comment on the most important experiments that led to the disputed announcement of the viroporin Vp4.
format	article
author	Stian Henriksen Christine Hanssen Rinaldo
author_facet	Stian Henriksen Christine Hanssen Rinaldo
author_sort	Stian Henriksen
title	Does the Evidence Support the Existence of the Simian Polyomavirus SV40 Vp4 Viroporin?
title_short	Does the Evidence Support the Existence of the Simian Polyomavirus SV40 Vp4 Viroporin?
title_full	Does the Evidence Support the Existence of the Simian Polyomavirus SV40 Vp4 Viroporin?
title_fullStr	Does the Evidence Support the Existence of the Simian Polyomavirus SV40 Vp4 Viroporin?
title_full_unstemmed	Does the Evidence Support the Existence of the Simian Polyomavirus SV40 Vp4 Viroporin?
title_sort	does the evidence support the existence of the simian polyomavirus sv40 vp4 viroporin?
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/508c88e75c524b7b90703fc4c783ce80
work_keys_str_mv	AT stianhenriksen doestheevidencesupporttheexistenceofthesimianpolyomavirussv40vp4viroporin AT christinehanssenrinaldo doestheevidencesupporttheexistenceofthesimianpolyomavirussv40vp4viroporin
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Does the Evidence Support the Existence of the Simian Polyomavirus SV40 Vp4 Viroporin?

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