Genome-Wide Characterization of Glutamine Synthetase Family Genes in <i>Cucurbitaceae</i> and Their Potential Roles in Cold Response and Rootstock-Scion Signaling Communication

Glutamine synthetase (GS; EC 6.3.1.2, L-glutamate: ammonia ligase ADP-forming) is the key enzyme responsible for the primary assimilation and reassimilation of nitrogen (N) in higher plants. There are two main isoforms of GS in higher plants, classified as cytosolic GS (GS1) and chloroplastic GS (GS...

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Autores principales:	Xiaojun Li, Xiaohong Lu, Mengshuang Liu, Chenggang Xiang, Wenqian Liu, Cuicui Wang, Xiaojing Zhang, Tao Wang, Zixi Liu, Lihong Gao, Wenna Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	genome-wide glutamine synthetase <i>Cucurbita moschata</i> expression profile phylogeny rootstock breeding Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/508d7b02552646748d5c8728779e2752
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spelling	oai:doaj.org-article:508d7b02552646748d5c8728779e27522021-11-25T16:01:06ZGenome-Wide Characterization of Glutamine Synthetase Family Genes in <i>Cucurbitaceae</i> and Their Potential Roles in Cold Response and Rootstock-Scion Signaling Communication10.3390/agriculture111111562077-0472https://doaj.org/article/508d7b02552646748d5c8728779e27522021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0472/11/11/1156https://doaj.org/toc/2077-0472Glutamine synthetase (GS; EC 6.3.1.2, L-glutamate: ammonia ligase ADP-forming) is the key enzyme responsible for the primary assimilation and reassimilation of nitrogen (N) in higher plants. There are two main isoforms of GS in higher plants, classified as cytosolic GS (GS1) and chloroplastic GS (GS2) by their size and subcellular localization. In order to improve the stress tolerance, quality, and yield of cucurbit crops such as cucumbers (Csa, <i>Cucumis sativus</i> L.), pumpkins (Cmo, <i>Cucurbita moschata</i> var. Rifu) are often used as rootstocks. Here, the GS family of the two species were comprehensively analyzed using bioinformatics in terms of aspects of the phylogenic tree, gene structure, chromosome location, subcellular localization, and evolutionary and expression patterns. Seven and four <i>GS</i> gene family members were screened in pumpkin and cucumber, respectively. <i>GS</i> family genes were divided into three groups (one for <i>GS2</i> and two for <i>GS1</i>) according to their homology and phylogenetic relationships with other species. The analysis of gene ontology annotation of <i>GS</i> family genes, promoter regulatory elements, and tissue-specific expression patterns indicates the potential different biological roles of GS isoforms in <i>Cucurbitaceae</i>. In particular, we have identified a potentially available gene (<i>GS</i>1: CmoCh08G004920) from pumpkin that is relatively highly expressed and tissue-specifically expressed. RT-PCR analysis showed that most CmoGSs are induced by low temperature, and long-term (day 2 to day 9) cold stress has a more obvious effect on the RNA abundance of CmoGS. Our work presents the structure and expression patterns of all candidate members of the pumpkin and cucumber <i>GS</i> gene family, and to the best of our knowledge, this is the first time such work has been presented. It is worth focusing on the candidate genes with strong capacity for improving pumpkin rootstock breeding in order to increase nitrogen-use efficiency in cold conditions, as well as rootstock-scion communication.Xiaojun LiXiaohong LuMengshuang LiuChenggang XiangWenqian LiuCuicui WangXiaojing ZhangTao WangZixi LiuLihong GaoWenna ZhangMDPI AGarticlegenome-wideglutamine synthetase<i>Cucurbita moschata</i>expression profilephylogenyrootstock breedingAgriculture (General)S1-972ENAgriculture, Vol 11, Iss 1156, p 1156 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	genome-wide glutamine synthetase <i>Cucurbita moschata</i> expression profile phylogeny rootstock breeding Agriculture (General) S1-972
spellingShingle	genome-wide glutamine synthetase <i>Cucurbita moschata</i> expression profile phylogeny rootstock breeding Agriculture (General) S1-972 Xiaojun Li Xiaohong Lu Mengshuang Liu Chenggang Xiang Wenqian Liu Cuicui Wang Xiaojing Zhang Tao Wang Zixi Liu Lihong Gao Wenna Zhang Genome-Wide Characterization of Glutamine Synthetase Family Genes in <i>Cucurbitaceae</i> and Their Potential Roles in Cold Response and Rootstock-Scion Signaling Communication
description	Glutamine synthetase (GS; EC 6.3.1.2, L-glutamate: ammonia ligase ADP-forming) is the key enzyme responsible for the primary assimilation and reassimilation of nitrogen (N) in higher plants. There are two main isoforms of GS in higher plants, classified as cytosolic GS (GS1) and chloroplastic GS (GS2) by their size and subcellular localization. In order to improve the stress tolerance, quality, and yield of cucurbit crops such as cucumbers (Csa, <i>Cucumis sativus</i> L.), pumpkins (Cmo, <i>Cucurbita moschata</i> var. Rifu) are often used as rootstocks. Here, the GS family of the two species were comprehensively analyzed using bioinformatics in terms of aspects of the phylogenic tree, gene structure, chromosome location, subcellular localization, and evolutionary and expression patterns. Seven and four <i>GS</i> gene family members were screened in pumpkin and cucumber, respectively. <i>GS</i> family genes were divided into three groups (one for <i>GS2</i> and two for <i>GS1</i>) according to their homology and phylogenetic relationships with other species. The analysis of gene ontology annotation of <i>GS</i> family genes, promoter regulatory elements, and tissue-specific expression patterns indicates the potential different biological roles of GS isoforms in <i>Cucurbitaceae</i>. In particular, we have identified a potentially available gene (<i>GS</i>1: CmoCh08G004920) from pumpkin that is relatively highly expressed and tissue-specifically expressed. RT-PCR analysis showed that most CmoGSs are induced by low temperature, and long-term (day 2 to day 9) cold stress has a more obvious effect on the RNA abundance of CmoGS. Our work presents the structure and expression patterns of all candidate members of the pumpkin and cucumber <i>GS</i> gene family, and to the best of our knowledge, this is the first time such work has been presented. It is worth focusing on the candidate genes with strong capacity for improving pumpkin rootstock breeding in order to increase nitrogen-use efficiency in cold conditions, as well as rootstock-scion communication.
format	article
author	Xiaojun Li Xiaohong Lu Mengshuang Liu Chenggang Xiang Wenqian Liu Cuicui Wang Xiaojing Zhang Tao Wang Zixi Liu Lihong Gao Wenna Zhang
author_facet	Xiaojun Li Xiaohong Lu Mengshuang Liu Chenggang Xiang Wenqian Liu Cuicui Wang Xiaojing Zhang Tao Wang Zixi Liu Lihong Gao Wenna Zhang
author_sort	Xiaojun Li
title	Genome-Wide Characterization of Glutamine Synthetase Family Genes in <i>Cucurbitaceae</i> and Their Potential Roles in Cold Response and Rootstock-Scion Signaling Communication
title_short	Genome-Wide Characterization of Glutamine Synthetase Family Genes in <i>Cucurbitaceae</i> and Their Potential Roles in Cold Response and Rootstock-Scion Signaling Communication
title_full	Genome-Wide Characterization of Glutamine Synthetase Family Genes in <i>Cucurbitaceae</i> and Their Potential Roles in Cold Response and Rootstock-Scion Signaling Communication
title_fullStr	Genome-Wide Characterization of Glutamine Synthetase Family Genes in <i>Cucurbitaceae</i> and Their Potential Roles in Cold Response and Rootstock-Scion Signaling Communication
title_full_unstemmed	Genome-Wide Characterization of Glutamine Synthetase Family Genes in <i>Cucurbitaceae</i> and Their Potential Roles in Cold Response and Rootstock-Scion Signaling Communication
title_sort	genome-wide characterization of glutamine synthetase family genes in <i>cucurbitaceae</i> and their potential roles in cold response and rootstock-scion signaling communication
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/508d7b02552646748d5c8728779e2752
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Genome-Wide Characterization of Glutamine Synthetase Family Genes in <i>Cucurbitaceae</i> and Their Potential Roles in Cold Response and Rootstock-Scion Signaling Communication

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