Short-lived detection of an introduced vertebrate eDNA signal in a nearshore rocky reef environment.

Environmental DNA (eDNA) is increasingly used to measure biodiversity of marine ecosystems, yet key aspects of the temporal dynamics of eDNA remain unknown. Of particular interest is in situ persistence of eDNA signals in dynamic marine environments, as eDNA degradation rates have predominantly been...

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Autores principales:	Taylor Ely, Paul H Barber, Lauren Man, Zachary Gold
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/509f7569bfcd419f907e916434985126
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spelling	oai:doaj.org-article:509f7569bfcd419f907e9164349851262021-12-02T20:03:56ZShort-lived detection of an introduced vertebrate eDNA signal in a nearshore rocky reef environment.1932-620310.1371/journal.pone.0245314https://doaj.org/article/509f7569bfcd419f907e9164349851262021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0245314https://doaj.org/toc/1932-6203Environmental DNA (eDNA) is increasingly used to measure biodiversity of marine ecosystems, yet key aspects of the temporal dynamics of eDNA remain unknown. Of particular interest is in situ persistence of eDNA signals in dynamic marine environments, as eDNA degradation rates have predominantly been quantified through mesocosm studies. To determine in situ eDNA residence times, we introduced an eDNA signal from a non-native fish into a protected bay of a Southern California rocky reef ecosystem, and then measured changes in both introduced and background eDNA signals across a fixed transect over 96 hours. Foreign eDNA signal was no longer detected only 7.5 hours after introduction, a time substantially shorter than the multi-day persistence times in laboratory studies. Moreover, the foreign eDNA signal spread along the entire 38 m transect within 1.5 hours after introduction, indicating that transport and diffusion play a role in eDNA detectability even in protected low energy marine environments. Similarly, native vertebrate eDNA signals varied greatly over the 96 hours of observation as well as within two additional nearby fixed transects sampled over 120 hours. While community structure did significantly change across time of day and tidal direction, neither accounted for the majority of observed variation. Combined, results show that both foreign and native eDNA signatures can exhibit substantial temporal heterogeneity, even on hourly time scales. Further work exploring eDNA decay from lagrangian perspective and quantifying effects of sample and technical replication are needed to better understand temporal variation of eDNA signatures in nearshore marine environments.Taylor ElyPaul H BarberLauren ManZachary GoldPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0245314 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Taylor Ely Paul H Barber Lauren Man Zachary Gold Short-lived detection of an introduced vertebrate eDNA signal in a nearshore rocky reef environment.
description	Environmental DNA (eDNA) is increasingly used to measure biodiversity of marine ecosystems, yet key aspects of the temporal dynamics of eDNA remain unknown. Of particular interest is in situ persistence of eDNA signals in dynamic marine environments, as eDNA degradation rates have predominantly been quantified through mesocosm studies. To determine in situ eDNA residence times, we introduced an eDNA signal from a non-native fish into a protected bay of a Southern California rocky reef ecosystem, and then measured changes in both introduced and background eDNA signals across a fixed transect over 96 hours. Foreign eDNA signal was no longer detected only 7.5 hours after introduction, a time substantially shorter than the multi-day persistence times in laboratory studies. Moreover, the foreign eDNA signal spread along the entire 38 m transect within 1.5 hours after introduction, indicating that transport and diffusion play a role in eDNA detectability even in protected low energy marine environments. Similarly, native vertebrate eDNA signals varied greatly over the 96 hours of observation as well as within two additional nearby fixed transects sampled over 120 hours. While community structure did significantly change across time of day and tidal direction, neither accounted for the majority of observed variation. Combined, results show that both foreign and native eDNA signatures can exhibit substantial temporal heterogeneity, even on hourly time scales. Further work exploring eDNA decay from lagrangian perspective and quantifying effects of sample and technical replication are needed to better understand temporal variation of eDNA signatures in nearshore marine environments.
format	article
author	Taylor Ely Paul H Barber Lauren Man Zachary Gold
author_facet	Taylor Ely Paul H Barber Lauren Man Zachary Gold
author_sort	Taylor Ely
title	Short-lived detection of an introduced vertebrate eDNA signal in a nearshore rocky reef environment.
title_short	Short-lived detection of an introduced vertebrate eDNA signal in a nearshore rocky reef environment.
title_full	Short-lived detection of an introduced vertebrate eDNA signal in a nearshore rocky reef environment.
title_fullStr	Short-lived detection of an introduced vertebrate eDNA signal in a nearshore rocky reef environment.
title_full_unstemmed	Short-lived detection of an introduced vertebrate eDNA signal in a nearshore rocky reef environment.
title_sort	short-lived detection of an introduced vertebrate edna signal in a nearshore rocky reef environment.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/509f7569bfcd419f907e916434985126
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Short-lived detection of an introduced vertebrate eDNA signal in a nearshore rocky reef environment.

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