Solution structure of the major fish allergen parvalbumin Sco j 1 derived from the Pacific mackerel

Abstract Although fish is an important part of the human diet, it is also a common source of food allergy. The major allergen in fish is parvalbumin, a well-conserved Ca2+-binding protein found in the white muscle of many fish species. Here, we studied the solution structure of the parvalbumin Sco j...

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Autores principales:	Hiroyuki Kumeta, Haruka Nakayama, Kenji Ogura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/509fb3e6bfff4ba0852209bf3ff83bf0
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spelling	oai:doaj.org-article:509fb3e6bfff4ba0852209bf3ff83bf02021-12-02T15:05:02ZSolution structure of the major fish allergen parvalbumin Sco j 1 derived from the Pacific mackerel10.1038/s41598-017-17281-62045-2322https://doaj.org/article/509fb3e6bfff4ba0852209bf3ff83bf02017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17281-6https://doaj.org/toc/2045-2322Abstract Although fish is an important part of the human diet, it is also a common source of food allergy. The major allergen in fish is parvalbumin, a well-conserved Ca2+-binding protein found in the white muscle of many fish species. Here, we studied the solution structure of the parvalbumin Sco j 1, derived from the Pacific mackerel, using nuclear magnetic resonance spectroscopy. We mapped the IgE-binding epitope proposed in a recent study onto the present structure. Interestingly, three of four residues, which were elucidated as key residues of the IgE-binding epitope, were exposed to solvent, whereas one residue faced the inside of the molecule. We expect that this solution structure can be used in future studies attempting to analyze the various IgE-binding modes of these allergens.Hiroyuki KumetaHaruka NakayamaKenji OguraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroyuki Kumeta Haruka Nakayama Kenji Ogura Solution structure of the major fish allergen parvalbumin Sco j 1 derived from the Pacific mackerel
description	Abstract Although fish is an important part of the human diet, it is also a common source of food allergy. The major allergen in fish is parvalbumin, a well-conserved Ca2+-binding protein found in the white muscle of many fish species. Here, we studied the solution structure of the parvalbumin Sco j 1, derived from the Pacific mackerel, using nuclear magnetic resonance spectroscopy. We mapped the IgE-binding epitope proposed in a recent study onto the present structure. Interestingly, three of four residues, which were elucidated as key residues of the IgE-binding epitope, were exposed to solvent, whereas one residue faced the inside of the molecule. We expect that this solution structure can be used in future studies attempting to analyze the various IgE-binding modes of these allergens.
format	article
author	Hiroyuki Kumeta Haruka Nakayama Kenji Ogura
author_facet	Hiroyuki Kumeta Haruka Nakayama Kenji Ogura
author_sort	Hiroyuki Kumeta
title	Solution structure of the major fish allergen parvalbumin Sco j 1 derived from the Pacific mackerel
title_short	Solution structure of the major fish allergen parvalbumin Sco j 1 derived from the Pacific mackerel
title_full	Solution structure of the major fish allergen parvalbumin Sco j 1 derived from the Pacific mackerel
title_fullStr	Solution structure of the major fish allergen parvalbumin Sco j 1 derived from the Pacific mackerel
title_full_unstemmed	Solution structure of the major fish allergen parvalbumin Sco j 1 derived from the Pacific mackerel
title_sort	solution structure of the major fish allergen parvalbumin sco j 1 derived from the pacific mackerel
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/509fb3e6bfff4ba0852209bf3ff83bf0
work_keys_str_mv	AT hiroyukikumeta solutionstructureofthemajorfishallergenparvalbuminscoj1derivedfromthepacificmackerel AT harukanakayama solutionstructureofthemajorfishallergenparvalbuminscoj1derivedfromthepacificmackerel AT kenjiogura solutionstructureofthemajorfishallergenparvalbuminscoj1derivedfromthepacificmackerel
_version_	1718388954130022400

Solution structure of the major fish allergen parvalbumin Sco j 1 derived from the Pacific mackerel

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