Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets

Summary: The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands o...

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Autores principales:	Daiyu Ito, Teruhiko Wakayama
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biotechnology and bioengineering Developmental biology Model Organisms Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/50a1d49e772549db93ceae839ebd4537
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spelling	oai:doaj.org-article:50a1d49e772549db93ceae839ebd45372021-11-06T04:36:04ZProtocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets2666-166710.1016/j.xpro.2021.100933https://doaj.org/article/50a1d49e772549db93ceae839ebd45372021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006390https://doaj.org/toc/2666-1667Summary: The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands of mouse strains in a card folder. We can also send the FD sperm domestically using a postcard without any extra equipment.For complete details on the use and execution of this protocol, please refer to Ito et al. (2021).Daiyu ItoTeruhiko WakayamaElsevierarticleBiotechnology and bioengineeringDevelopmental biologyModel OrganismsScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100933- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology and bioengineering Developmental biology Model Organisms Science (General) Q1-390
spellingShingle	Biotechnology and bioengineering Developmental biology Model Organisms Science (General) Q1-390 Daiyu Ito Teruhiko Wakayama Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
description	Summary: The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands of mouse strains in a card folder. We can also send the FD sperm domestically using a postcard without any extra equipment.For complete details on the use and execution of this protocol, please refer to Ito et al. (2021).
format	article
author	Daiyu Ito Teruhiko Wakayama
author_facet	Daiyu Ito Teruhiko Wakayama
author_sort	Daiyu Ito
title	Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title_short	Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title_full	Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title_fullStr	Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title_full_unstemmed	Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
title_sort	protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/50a1d49e772549db93ceae839ebd4537
work_keys_str_mv	AT daiyuito protocoltopreservemousefreezedriedspermatozoainthethinplasticsheets AT teruhikowakayama protocoltopreservemousefreezedriedspermatozoainthethinplasticsheets
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Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets

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