Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets

Summary: The preservation of mammalian freeze-dried (FD) spermatozoa is commonly performed using small glass ampules; however, they are bulky and breakable. In this study, we present a protocol to prepare and preserve mouse FD sperm using thin plastic sheets. This approach allows storing thousands o...

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Autores principales: Daiyu Ito, Teruhiko Wakayama
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
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Acceso en línea:https://doaj.org/article/50a1d49e772549db93ceae839ebd4537
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