Inflammatory cytokines regulate endothelial cell survival and tissue repair functions via NF-κB signaling
Nobuhiro Kanaji1, Tadashi Sato2, Amy Nelson3, Xingqi Wang3, YingJi Li4, Miok Kim5, Masanori Nakanishi6, Hesham Basma3, Joel Michalski3, Maha Farid3, Michael Chandler3, William Pease3, Amol Patil3, Stephen I Rennard3, Xiangde Liu31Division of Hematology, Rheumatology and Respiratory Medicine, Kagawa...
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Dove Medical Press
2011
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oai:doaj.org-article:50a49140d6524926acb336abc9dd45552021-12-02T00:50:56ZInflammatory cytokines regulate endothelial cell survival and tissue repair functions via NF-κB signaling1178-7031https://doaj.org/article/50a49140d6524926acb336abc9dd45552011-09-01T00:00:00Zhttp://www.dovepress.com/inflammatory-cytokines-regulate-endothelial-cell-survival-and-tissue-r-a8291https://doaj.org/toc/1178-7031Nobuhiro Kanaji1, Tadashi Sato2, Amy Nelson3, Xingqi Wang3, YingJi Li4, Miok Kim5, Masanori Nakanishi6, Hesham Basma3, Joel Michalski3, Maha Farid3, Michael Chandler3, William Pease3, Amol Patil3, Stephen I Rennard3, Xiangde Liu31Division of Hematology, Rheumatology and Respiratory Medicine, Kagawa University, Kagawa, Japan; 2Department of Respiratory Medicine, Juntendo University School of Medicine, Tokyo, Japan; 3Pulmonary and Critical Care Medicine, University of Nebraska Medical Center, Omaha, Nebraska; 4Department of Hygiene and Public Health, Nippon Medical School, Tokyo, Japan; 5Third Department of Internal Medicine, Wakayama Medical University School of Medicine, Wakayama, Japan; 6Department of Internal Medicine, Jeju Medical College, Jeju, Republic of KoreaAbstract: Inflammation contributes to the development of fibrotic and malignant diseases. We assessed the ability of inflammatory cytokines to modulate endothelial cell survival and functions related to tissue repair/remodeling. Treatment with interleukin (IL)-1ß or tumor necrosis factor (TNF)-α (2 ng/mL) led to human pulmonary artery endothelial cells becoming spindle-shaped fibroblast-like cells. However, immunoblot and DNA microarray showed no change in most endothelial and mesenchymal markers. In the presence of IL-1ß or TNF-α, cells were resistant to apoptosis induced by deprivation of serum and growth factor, and were more migratory. In addition, cells treated with IL-1ß or TNF-α contracted collagen gels more robustly. In contrast, transforming growth factor-ß1 did not induce these responses. RNA interference targeting nuclear factor (NF)- κB p65 blocked the effects of IL-1ß or TNF-α on cell morphologic change, survival, migration, and collagen gel contraction. These results suggest that endothelial cells may contribute to tissue repair/remodeling via the NF-κB signaling in a milieu of airway inflammation.Keywords: NF-κB, IL-1ß, TNF-α, apoptosis, tissue repairKanaji NSato TNelson AWang XLi YKim MNakanishi MBasma HMichalski JFarid MChandler MPease WPatil ARennard SILiu XDove Medical PressarticlePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2011, Iss default, Pp 127-138 (2011) |
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Pathology RB1-214 Therapeutics. Pharmacology RM1-950 |
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Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Kanaji N Sato T Nelson A Wang X Li Y Kim M Nakanishi M Basma H Michalski J Farid M Chandler M Pease W Patil A Rennard SI Liu X Inflammatory cytokines regulate endothelial cell survival and tissue repair functions via NF-κB signaling |
| description |
Nobuhiro Kanaji1, Tadashi Sato2, Amy Nelson3, Xingqi Wang3, YingJi Li4, Miok Kim5, Masanori Nakanishi6, Hesham Basma3, Joel Michalski3, Maha Farid3, Michael Chandler3, William Pease3, Amol Patil3, Stephen I Rennard3, Xiangde Liu31Division of Hematology, Rheumatology and Respiratory Medicine, Kagawa University, Kagawa, Japan; 2Department of Respiratory Medicine, Juntendo University School of Medicine, Tokyo, Japan; 3Pulmonary and Critical Care Medicine, University of Nebraska Medical Center, Omaha, Nebraska; 4Department of Hygiene and Public Health, Nippon Medical School, Tokyo, Japan; 5Third Department of Internal Medicine, Wakayama Medical University School of Medicine, Wakayama, Japan; 6Department of Internal Medicine, Jeju Medical College, Jeju, Republic of KoreaAbstract: Inflammation contributes to the development of fibrotic and malignant diseases. We assessed the ability of inflammatory cytokines to modulate endothelial cell survival and functions related to tissue repair/remodeling. Treatment with interleukin (IL)-1ß or tumor necrosis factor (TNF)-α (2 ng/mL) led to human pulmonary artery endothelial cells becoming spindle-shaped fibroblast-like cells. However, immunoblot and DNA microarray showed no change in most endothelial and mesenchymal markers. In the presence of IL-1ß or TNF-α, cells were resistant to apoptosis induced by deprivation of serum and growth factor, and were more migratory. In addition, cells treated with IL-1ß or TNF-α contracted collagen gels more robustly. In contrast, transforming growth factor-ß1 did not induce these responses. RNA interference targeting nuclear factor (NF)- κB p65 blocked the effects of IL-1ß or TNF-α on cell morphologic change, survival, migration, and collagen gel contraction. These results suggest that endothelial cells may contribute to tissue repair/remodeling via the NF-κB signaling in a milieu of airway inflammation.Keywords: NF-κB, IL-1ß, TNF-α, apoptosis, tissue repair |
| format |
article |
| author |
Kanaji N Sato T Nelson A Wang X Li Y Kim M Nakanishi M Basma H Michalski J Farid M Chandler M Pease W Patil A Rennard SI Liu X |
| author_facet |
Kanaji N Sato T Nelson A Wang X Li Y Kim M Nakanishi M Basma H Michalski J Farid M Chandler M Pease W Patil A Rennard SI Liu X |
| author_sort |
Kanaji N |
| title |
Inflammatory cytokines regulate endothelial cell survival and tissue repair functions via NF-κB signaling |
| title_short |
Inflammatory cytokines regulate endothelial cell survival and tissue repair functions via NF-κB signaling |
| title_full |
Inflammatory cytokines regulate endothelial cell survival and tissue repair functions via NF-κB signaling |
| title_fullStr |
Inflammatory cytokines regulate endothelial cell survival and tissue repair functions via NF-κB signaling |
| title_full_unstemmed |
Inflammatory cytokines regulate endothelial cell survival and tissue repair functions via NF-κB signaling |
| title_sort |
inflammatory cytokines regulate endothelial cell survival and tissue repair functions via nf-κb signaling |
| publisher |
Dove Medical Press |
| publishDate |
2011 |
| url |
https://doaj.org/article/50a49140d6524926acb336abc9dd4555 |
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