Crowdsourced analysis of fungal growth and branching on microfluidic platforms.
Fungal hyphal growth and branching are essential traits that allow fungi to spread and proliferate in many environments. This sustained growth is essential for a myriad of applications in health, agriculture, and industry. However, comparisons between different fungi are difficult in the absence of...
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Public Library of Science (PLoS)
2021
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oai:doaj.org-article:50a52dd44e9b41b2b2cff5b4117efc3d2021-12-02T20:14:01ZCrowdsourced analysis of fungal growth and branching on microfluidic platforms.1932-620310.1371/journal.pone.0257823https://doaj.org/article/50a52dd44e9b41b2b2cff5b4117efc3d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257823https://doaj.org/toc/1932-6203Fungal hyphal growth and branching are essential traits that allow fungi to spread and proliferate in many environments. This sustained growth is essential for a myriad of applications in health, agriculture, and industry. However, comparisons between different fungi are difficult in the absence of standardized metrics. Here, we used a microfluidic device featuring four different maze patterns to compare the growth velocity and branching frequency of fourteen filamentous fungi. These measurements result from the collective work of several labs in the form of a competition named the "Fungus Olympics." The competing fungi included five ascomycete species (ten strains total), two basidiomycete species, and two zygomycete species. We found that growth velocity within a straight channel varied from 1 to 4 μm/min. We also found that the time to complete mazes when fungal hyphae branched or turned at various angles did not correlate with linear growth velocity. We discovered that fungi in our study used one of two distinct strategies to traverse mazes: high-frequency branching in which all possible paths were explored, and low-frequency branching in which only one or two paths were explored. While the high-frequency branching helped fungi escape mazes with sharp turns faster, the low-frequency turning had a significant advantage in mazes with shallower turns. Future work will more systematically examine these trends.Alex HopkeAlex MelaFelix EllettDerreck Carter-HouseJesús F PeñaJason E StajichSophie AltamiranoBrian LovettMartin EganShiv KaleIlkka KronholmPaul GueretteEdyta SzewczykKevin McCluskeyDavid BreslauerHiral ShahBryan R CoadMichelle MomanyDaniel IrimiaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257823 (2021) |
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Medicine R Science Q |
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Medicine R Science Q Alex Hopke Alex Mela Felix Ellett Derreck Carter-House Jesús F Peña Jason E Stajich Sophie Altamirano Brian Lovett Martin Egan Shiv Kale Ilkka Kronholm Paul Guerette Edyta Szewczyk Kevin McCluskey David Breslauer Hiral Shah Bryan R Coad Michelle Momany Daniel Irimia Crowdsourced analysis of fungal growth and branching on microfluidic platforms. |
| description |
Fungal hyphal growth and branching are essential traits that allow fungi to spread and proliferate in many environments. This sustained growth is essential for a myriad of applications in health, agriculture, and industry. However, comparisons between different fungi are difficult in the absence of standardized metrics. Here, we used a microfluidic device featuring four different maze patterns to compare the growth velocity and branching frequency of fourteen filamentous fungi. These measurements result from the collective work of several labs in the form of a competition named the "Fungus Olympics." The competing fungi included five ascomycete species (ten strains total), two basidiomycete species, and two zygomycete species. We found that growth velocity within a straight channel varied from 1 to 4 μm/min. We also found that the time to complete mazes when fungal hyphae branched or turned at various angles did not correlate with linear growth velocity. We discovered that fungi in our study used one of two distinct strategies to traverse mazes: high-frequency branching in which all possible paths were explored, and low-frequency branching in which only one or two paths were explored. While the high-frequency branching helped fungi escape mazes with sharp turns faster, the low-frequency turning had a significant advantage in mazes with shallower turns. Future work will more systematically examine these trends. |
| format |
article |
| author |
Alex Hopke Alex Mela Felix Ellett Derreck Carter-House Jesús F Peña Jason E Stajich Sophie Altamirano Brian Lovett Martin Egan Shiv Kale Ilkka Kronholm Paul Guerette Edyta Szewczyk Kevin McCluskey David Breslauer Hiral Shah Bryan R Coad Michelle Momany Daniel Irimia |
| author_facet |
Alex Hopke Alex Mela Felix Ellett Derreck Carter-House Jesús F Peña Jason E Stajich Sophie Altamirano Brian Lovett Martin Egan Shiv Kale Ilkka Kronholm Paul Guerette Edyta Szewczyk Kevin McCluskey David Breslauer Hiral Shah Bryan R Coad Michelle Momany Daniel Irimia |
| author_sort |
Alex Hopke |
| title |
Crowdsourced analysis of fungal growth and branching on microfluidic platforms. |
| title_short |
Crowdsourced analysis of fungal growth and branching on microfluidic platforms. |
| title_full |
Crowdsourced analysis of fungal growth and branching on microfluidic platforms. |
| title_fullStr |
Crowdsourced analysis of fungal growth and branching on microfluidic platforms. |
| title_full_unstemmed |
Crowdsourced analysis of fungal growth and branching on microfluidic platforms. |
| title_sort |
crowdsourced analysis of fungal growth and branching on microfluidic platforms. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/50a52dd44e9b41b2b2cff5b4117efc3d |
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