Claudin 14/15 play important roles in early wallerian degeneration after rat sciatic nerve injury

Purpose: Wallerian degeneration (WD) is an antegrade degenerative process distal to peripheral nerve injury. Numerous genes are differentially regulated in response to the process. However, the underlying mechanism is unclear, especially the early response. We aimed at investigating the effects of s...

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Autores principales:	Min Cai, Jian Shao, Yi Wang, Bryant Yung, Jian-Nan Li, Huan-Huan Zhang, Yu-Ting Li, Deng-Bing Yao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Nerve regeneration Schwann cells Sciatic nerve Tight junctions Wallerian degeneration Claudin 14/15 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/50b08c90e22d4446a0117359778c2ab2
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spelling	oai:doaj.org-article:50b08c90e22d4446a0117359778c2ab22021-11-18T04:43:42ZClaudin 14/15 play important roles in early wallerian degeneration after rat sciatic nerve injury1008-127510.1016/j.cjtee.2021.04.004https://doaj.org/article/50b08c90e22d4446a0117359778c2ab22021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1008127521000602https://doaj.org/toc/1008-1275Purpose: Wallerian degeneration (WD) is an antegrade degenerative process distal to peripheral nerve injury. Numerous genes are differentially regulated in response to the process. However, the underlying mechanism is unclear, especially the early response. We aimed at investigating the effects of sciatic nerve injury on WD via CLDN 14/15 interactions in vivo and in vitro. Methods: Using the methods of molecular biology and bioinformatics analysis, we investigated the molecular mechanism by which claudin 14/15 participate in WD. Our previous study showed that claudins 14 and 15 trigger the early signal flow and pathway in damaged sciatic nerves. Here, we report the effects of the interaction between claudin 14 and claudin 15 on nerve degeneration and regeneration during early WD. Results: It was found that claudin 14/15 were upregulated in the sciatic nerve in WD. Claudin 14/15 promoted Schwann cell proliferation, migration and anti-apoptosis in vitro. PKCα, NT3, NF2, and bFGF were significantly upregulated in transfected Schwann cells. Moreover, the expression levels of the β-catenin, p-AKT/AKT, p-c-jun/c-jun, and p-ERK/ERK signaling pathways were also significantly altered. Conclusion: Claudin 14/15 affect Schwann cell proliferation, migration, and anti-apoptosis via the β-catenin, p-AKT/AKT, p-c-jun/c-jun, and p-ERK/ERK pathways in vitro and in vivo. The results of this study may help elucidate the molecular mechanisms of the tight junction signaling pathway underlying peripheral nerve degeneration.Min CaiJian ShaoYi WangBryant YungJian-Nan LiHuan-Huan ZhangYu-Ting LiDeng-Bing YaoElsevierarticleNerve regenerationSchwann cellsSciatic nerveTight junctionsWallerian degenerationClaudin 14/15Medicine (General)R5-920ENChinese Journal of Traumatology, Vol 24, Iss 6, Pp 374-382 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nerve regeneration Schwann cells Sciatic nerve Tight junctions Wallerian degeneration Claudin 14/15 Medicine (General) R5-920
spellingShingle	Nerve regeneration Schwann cells Sciatic nerve Tight junctions Wallerian degeneration Claudin 14/15 Medicine (General) R5-920 Min Cai Jian Shao Yi Wang Bryant Yung Jian-Nan Li Huan-Huan Zhang Yu-Ting Li Deng-Bing Yao Claudin 14/15 play important roles in early wallerian degeneration after rat sciatic nerve injury
description	Purpose: Wallerian degeneration (WD) is an antegrade degenerative process distal to peripheral nerve injury. Numerous genes are differentially regulated in response to the process. However, the underlying mechanism is unclear, especially the early response. We aimed at investigating the effects of sciatic nerve injury on WD via CLDN 14/15 interactions in vivo and in vitro. Methods: Using the methods of molecular biology and bioinformatics analysis, we investigated the molecular mechanism by which claudin 14/15 participate in WD. Our previous study showed that claudins 14 and 15 trigger the early signal flow and pathway in damaged sciatic nerves. Here, we report the effects of the interaction between claudin 14 and claudin 15 on nerve degeneration and regeneration during early WD. Results: It was found that claudin 14/15 were upregulated in the sciatic nerve in WD. Claudin 14/15 promoted Schwann cell proliferation, migration and anti-apoptosis in vitro. PKCα, NT3, NF2, and bFGF were significantly upregulated in transfected Schwann cells. Moreover, the expression levels of the β-catenin, p-AKT/AKT, p-c-jun/c-jun, and p-ERK/ERK signaling pathways were also significantly altered. Conclusion: Claudin 14/15 affect Schwann cell proliferation, migration, and anti-apoptosis via the β-catenin, p-AKT/AKT, p-c-jun/c-jun, and p-ERK/ERK pathways in vitro and in vivo. The results of this study may help elucidate the molecular mechanisms of the tight junction signaling pathway underlying peripheral nerve degeneration.
format	article
author	Min Cai Jian Shao Yi Wang Bryant Yung Jian-Nan Li Huan-Huan Zhang Yu-Ting Li Deng-Bing Yao
author_facet	Min Cai Jian Shao Yi Wang Bryant Yung Jian-Nan Li Huan-Huan Zhang Yu-Ting Li Deng-Bing Yao
author_sort	Min Cai
title	Claudin 14/15 play important roles in early wallerian degeneration after rat sciatic nerve injury
title_short	Claudin 14/15 play important roles in early wallerian degeneration after rat sciatic nerve injury
title_full	Claudin 14/15 play important roles in early wallerian degeneration after rat sciatic nerve injury
title_fullStr	Claudin 14/15 play important roles in early wallerian degeneration after rat sciatic nerve injury
title_full_unstemmed	Claudin 14/15 play important roles in early wallerian degeneration after rat sciatic nerve injury
title_sort	claudin 14/15 play important roles in early wallerian degeneration after rat sciatic nerve injury
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/50b08c90e22d4446a0117359778c2ab2
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Claudin 14/15 play important roles in early wallerian degeneration after rat sciatic nerve injury

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