The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.

The capped Small segment mRNA (SmRNA) of the Andes orthohantavirus (ANDV) lacks a poly(A) tail. In this study, we characterize the mechanism driving ANDV-SmRNA translation. Results show that the ANDV-nucleocapsid protein (ANDV-N) promotes in vitro translation from capped mRNAs without replacing euka...

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Autores principales:	Jorge Vera-Otarola, Estefania Castillo-Vargas, Jenniffer Angulo, Francisco M Barriga, Eduard Batlle, Marcelo Lopez-Lastra
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/50b495b2106e46d983ccac4998ebde04
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spelling	oai:doaj.org-article:50b495b2106e46d983ccac4998ebde042021-12-02T20:00:08ZThe viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.1553-73661553-737410.1371/journal.ppat.1009931https://doaj.org/article/50b495b2106e46d983ccac4998ebde042021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009931https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The capped Small segment mRNA (SmRNA) of the Andes orthohantavirus (ANDV) lacks a poly(A) tail. In this study, we characterize the mechanism driving ANDV-SmRNA translation. Results show that the ANDV-nucleocapsid protein (ANDV-N) promotes in vitro translation from capped mRNAs without replacing eukaryotic initiation factor (eIF) 4G. Using an RNA affinity chromatography approach followed by mass spectrometry, we identify the human RNA chaperone Mex3A (hMex3A) as a SmRNA-3'UTR binding protein. Results show that hMex3A enhances SmRNA translation in a 3'UTR dependent manner, either alone or when co-expressed with the ANDV-N. The ANDV-N and hMex3A proteins do not interact in cells, but both proteins interact with eIF4G. The hMex3A-eIF4G interaction showed to be independent of ANDV-infection or ANDV-N expression. Together, our observations suggest that translation of the ANDV SmRNA is enhanced by a 5'-3' end interaction, mediated by both viral and cellular proteins.Jorge Vera-OtarolaEstefania Castillo-VargasJenniffer AnguloFrancisco M BarrigaEduard BatlleMarcelo Lopez-LastraPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 9, p e1009931 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Jorge Vera-Otarola Estefania Castillo-Vargas Jenniffer Angulo Francisco M Barriga Eduard Batlle Marcelo Lopez-Lastra The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.
description	The capped Small segment mRNA (SmRNA) of the Andes orthohantavirus (ANDV) lacks a poly(A) tail. In this study, we characterize the mechanism driving ANDV-SmRNA translation. Results show that the ANDV-nucleocapsid protein (ANDV-N) promotes in vitro translation from capped mRNAs without replacing eukaryotic initiation factor (eIF) 4G. Using an RNA affinity chromatography approach followed by mass spectrometry, we identify the human RNA chaperone Mex3A (hMex3A) as a SmRNA-3'UTR binding protein. Results show that hMex3A enhances SmRNA translation in a 3'UTR dependent manner, either alone or when co-expressed with the ANDV-N. The ANDV-N and hMex3A proteins do not interact in cells, but both proteins interact with eIF4G. The hMex3A-eIF4G interaction showed to be independent of ANDV-infection or ANDV-N expression. Together, our observations suggest that translation of the ANDV SmRNA is enhanced by a 5'-3' end interaction, mediated by both viral and cellular proteins.
format	article
author	Jorge Vera-Otarola Estefania Castillo-Vargas Jenniffer Angulo Francisco M Barriga Eduard Batlle Marcelo Lopez-Lastra
author_facet	Jorge Vera-Otarola Estefania Castillo-Vargas Jenniffer Angulo Francisco M Barriga Eduard Batlle Marcelo Lopez-Lastra
author_sort	Jorge Vera-Otarola
title	The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.
title_short	The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.
title_full	The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.
title_fullStr	The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.
title_full_unstemmed	The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.
title_sort	viral nucleocapsid protein and the human rna-binding protein mex3a promote translation of the andes orthohantavirus small mrna.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/50b495b2106e46d983ccac4998ebde04
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The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.

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