Evaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration

Abstract Mechanically ventilated (MV) patients may present airway inflammation and elevated secretion production. However, it is unknown whether cell and/or protein counts in bronchial samples may be useful to evaluate their clinical condition. Our aim was to standardize sampling and propose a new m...

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Autores principales:	Rodopi Stamatiou, Vasiliki Tsolaki, Apostolia Hatziefthimiou, Epaminondas Zakynthinos, Demosthenes Makris
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/50b698fd247a4f4b96008504348cc7ce
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spelling	oai:doaj.org-article:50b698fd247a4f4b96008504348cc7ce2021-12-02T18:01:40ZEvaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration10.1038/s41598-021-99262-42045-2322https://doaj.org/article/50b698fd247a4f4b96008504348cc7ce2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99262-4https://doaj.org/toc/2045-2322Abstract Mechanically ventilated (MV) patients may present airway inflammation and elevated secretion production. However, it is unknown whether cell and/or protein counts in bronchial samples may be useful to evaluate their clinical condition. Our aim was to standardize sampling and propose a new mechanical mucus dissolution in Tracheal-Bronchial secretions. In all patients, bronchial lining fluid aspiration (BLF), Bronchoalveolar lavage (BAL) and Bronchial Washings (BW40, BW5) were performed, while visible bronchial secretions were obtained via bronchoscopy (VBS) and blinded, via a common catheter for tracheobronchial aspiration (AC). Mucus was mechanically or DTT dissolved and cell number was count. Protein, albumin and TNF-α levels were measured, in mucus dissolved samples from control and MV patients. Cell number and protein levels were elevated in mucus dissolved compared to non-dissolved, or DTT dissolved. Cell number and TNF-α levels were elevated in MV patients compared to controls, while protein levels were lower in MV patients. Differences in cell and protein levels were observed in samples acquired using different sampling technics. Therefore, mechanical mucus dissolution provides a proper sample for evaluation, and the sampling technic used can influence the sample’s characteristics.Rodopi StamatiouVasiliki TsolakiApostolia HatziefthimiouEpaminondas ZakynthinosDemosthenes MakrisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rodopi Stamatiou Vasiliki Tsolaki Apostolia Hatziefthimiou Epaminondas Zakynthinos Demosthenes Makris Evaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration
description	Abstract Mechanically ventilated (MV) patients may present airway inflammation and elevated secretion production. However, it is unknown whether cell and/or protein counts in bronchial samples may be useful to evaluate their clinical condition. Our aim was to standardize sampling and propose a new mechanical mucus dissolution in Tracheal-Bronchial secretions. In all patients, bronchial lining fluid aspiration (BLF), Bronchoalveolar lavage (BAL) and Bronchial Washings (BW40, BW5) were performed, while visible bronchial secretions were obtained via bronchoscopy (VBS) and blinded, via a common catheter for tracheobronchial aspiration (AC). Mucus was mechanically or DTT dissolved and cell number was count. Protein, albumin and TNF-α levels were measured, in mucus dissolved samples from control and MV patients. Cell number and protein levels were elevated in mucus dissolved compared to non-dissolved, or DTT dissolved. Cell number and TNF-α levels were elevated in MV patients compared to controls, while protein levels were lower in MV patients. Differences in cell and protein levels were observed in samples acquired using different sampling technics. Therefore, mechanical mucus dissolution provides a proper sample for evaluation, and the sampling technic used can influence the sample’s characteristics.
format	article
author	Rodopi Stamatiou Vasiliki Tsolaki Apostolia Hatziefthimiou Epaminondas Zakynthinos Demosthenes Makris
author_facet	Rodopi Stamatiou Vasiliki Tsolaki Apostolia Hatziefthimiou Epaminondas Zakynthinos Demosthenes Makris
author_sort	Rodopi Stamatiou
title	Evaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration
title_short	Evaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration
title_full	Evaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration
title_fullStr	Evaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration
title_full_unstemmed	Evaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration
title_sort	evaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/50b698fd247a4f4b96008504348cc7ce
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Evaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration

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