AtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow

Annexins are a multifunctional class of calcium-binding proteins in plants, and their physiological functions and regulation in response to drought stress remain to be elucidated. Here, we found that AtANN2 and AtANN3 conferred to drought tolerance under short-day and long-day conditions, respective...

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Autores principales:	Ting Liu, Leyan Du, Qiushi Li, Jingda Kang, Qi Guo, Shilin Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	drought tolerance photoperiod calcium annexin cryptochrome Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/50b87d7bc9214bfe82a55e1844566bbc
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spelling	oai:doaj.org-article:50b87d7bc9214bfe82a55e1844566bbc2021-11-30T12:28:33ZAtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow1664-462X10.3389/fpls.2021.754567https://doaj.org/article/50b87d7bc9214bfe82a55e1844566bbc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.754567/fullhttps://doaj.org/toc/1664-462XAnnexins are a multifunctional class of calcium-binding proteins in plants, and their physiological functions and regulation in response to drought stress remain to be elucidated. Here, we found that AtANN2 and AtANN3 conferred to drought tolerance under short-day and long-day conditions, respectively. Under their functional photoperiod, AtANN2 and AtANN3 gene expression was enhanced in the mannitol-treated roots, and their encoded proteins were rapidly targeted to the plasma membrane, and mediated significant Ca2+ flows across the plasma membrane. Cryptochromes as photoreceptors can not only sense the photoperiod and regulate ion channels on the plasma membrane to influence ion flow but also induce downstream physiological responses. AtCRY2 repressed the functions of AtANN2 and AtANN3 by affecting their plasma membrane localization and inhibited AtANN2- and AtANN3-dependent transmembrane Ca2+ flow in response to drought stress. Taken together, these results uncover a mechanism linking Annexins-AtCRY2 to transmembrane Ca2+ flow and resulting in enhanced drought tolerance in Arabidopsis.Ting LiuTing LiuLeyan DuQiushi LiQiushi LiJingda KangQi GuoQi GuoShilin WangShilin WangFrontiers Media S.A.articledrought tolerancephotoperiodcalciumannexincryptochromePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	drought tolerance photoperiod calcium annexin cryptochrome Plant culture SB1-1110
spellingShingle	drought tolerance photoperiod calcium annexin cryptochrome Plant culture SB1-1110 Ting Liu Ting Liu Leyan Du Qiushi Li Qiushi Li Jingda Kang Qi Guo Qi Guo Shilin Wang Shilin Wang AtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow
description	Annexins are a multifunctional class of calcium-binding proteins in plants, and their physiological functions and regulation in response to drought stress remain to be elucidated. Here, we found that AtANN2 and AtANN3 conferred to drought tolerance under short-day and long-day conditions, respectively. Under their functional photoperiod, AtANN2 and AtANN3 gene expression was enhanced in the mannitol-treated roots, and their encoded proteins were rapidly targeted to the plasma membrane, and mediated significant Ca2+ flows across the plasma membrane. Cryptochromes as photoreceptors can not only sense the photoperiod and regulate ion channels on the plasma membrane to influence ion flow but also induce downstream physiological responses. AtCRY2 repressed the functions of AtANN2 and AtANN3 by affecting their plasma membrane localization and inhibited AtANN2- and AtANN3-dependent transmembrane Ca2+ flow in response to drought stress. Taken together, these results uncover a mechanism linking Annexins-AtCRY2 to transmembrane Ca2+ flow and resulting in enhanced drought tolerance in Arabidopsis.
format	article
author	Ting Liu Ting Liu Leyan Du Qiushi Li Qiushi Li Jingda Kang Qi Guo Qi Guo Shilin Wang Shilin Wang
author_facet	Ting Liu Ting Liu Leyan Du Qiushi Li Qiushi Li Jingda Kang Qi Guo Qi Guo Shilin Wang Shilin Wang
author_sort	Ting Liu
title	AtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow
title_short	AtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow
title_full	AtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow
title_fullStr	AtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow
title_full_unstemmed	AtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow
title_sort	atcry2 negatively regulates the functions of atann2 and atann3 in drought tolerance by affecting their subcellular localization and transmembrane ca2+ flow
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/50b87d7bc9214bfe82a55e1844566bbc
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AtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow

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