Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia

ABSTRACT Tilapia are an important global food source due to their omnivorous diet, tolerance for high-density aquaculture, and relative disease resistance. Since 2009, tilapia aquaculture has been threatened by mass die-offs in farmed fish in Israel and Ecuador. Here we report evidence implicating a...

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Autores principales:	Eran Bacharach, Nischay Mishra, Thomas Briese, Michael C. Zody, Japhette Esther Kembou Tsofack, Rachel Zamostiano, Asaf Berkowitz, James Ng, Adam Nitido, André Corvelo, Nora C. Toussaint, Sandra Cathrine Abel Nielsen, Mady Hornig, Jorge Del Pozo, Toby Bloom, Hugh Ferguson, Avi Eldar, W. Ian Lipkin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/50b88d96a40f4c0ead256d47f3512cd7
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spelling	oai:doaj.org-article:50b88d96a40f4c0ead256d47f3512cd72021-11-15T15:41:41ZCharacterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia10.1128/mBio.00431-162150-7511https://doaj.org/article/50b88d96a40f4c0ead256d47f3512cd72016-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00431-16https://doaj.org/toc/2150-7511ABSTRACT Tilapia are an important global food source due to their omnivorous diet, tolerance for high-density aquaculture, and relative disease resistance. Since 2009, tilapia aquaculture has been threatened by mass die-offs in farmed fish in Israel and Ecuador. Here we report evidence implicating a novel orthomyxo-like virus in these outbreaks. The tilapia lake virus (TiLV) has a 10-segment, negative-sense RNA genome. The largest segment, segment 1, contains an open reading frame with weak sequence homology to the influenza C virus PB1 subunit. The other nine segments showed no homology to other viruses but have conserved, complementary sequences at their 5′ and 3′ termini, consistent with the genome organization found in other orthomyxoviruses. In situ hybridization indicates TiLV replication and transcription at sites of pathology in the liver and central nervous system of tilapia with disease. IMPORTANCE The economic impact of worldwide trade in tilapia is estimated at $7.5 billion U.S. dollars (USD) annually. The infectious agent implicated in mass tilapia die-offs in two continents poses a threat to the global tilapia industry, which not only provides inexpensive dietary protein but also is a major employer in the developing world. Here we report characterization of the causative agent as a novel orthomyxo-like virus, tilapia lake virus (TiLV). We also describe complete genomic and protein sequences that will facilitate TiLV detection and containment and enable vaccine development.Eran BacharachNischay MishraThomas BrieseMichael C. ZodyJaphette Esther Kembou TsofackRachel ZamostianoAsaf BerkowitzJames NgAdam NitidoAndré CorveloNora C. ToussaintSandra Cathrine Abel NielsenMady HornigJorge Del PozoToby BloomHugh FergusonAvi EldarW. Ian LipkinAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Eran Bacharach Nischay Mishra Thomas Briese Michael C. Zody Japhette Esther Kembou Tsofack Rachel Zamostiano Asaf Berkowitz James Ng Adam Nitido André Corvelo Nora C. Toussaint Sandra Cathrine Abel Nielsen Mady Hornig Jorge Del Pozo Toby Bloom Hugh Ferguson Avi Eldar W. Ian Lipkin Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia
description	ABSTRACT Tilapia are an important global food source due to their omnivorous diet, tolerance for high-density aquaculture, and relative disease resistance. Since 2009, tilapia aquaculture has been threatened by mass die-offs in farmed fish in Israel and Ecuador. Here we report evidence implicating a novel orthomyxo-like virus in these outbreaks. The tilapia lake virus (TiLV) has a 10-segment, negative-sense RNA genome. The largest segment, segment 1, contains an open reading frame with weak sequence homology to the influenza C virus PB1 subunit. The other nine segments showed no homology to other viruses but have conserved, complementary sequences at their 5′ and 3′ termini, consistent with the genome organization found in other orthomyxoviruses. In situ hybridization indicates TiLV replication and transcription at sites of pathology in the liver and central nervous system of tilapia with disease. IMPORTANCE The economic impact of worldwide trade in tilapia is estimated at $7.5 billion U.S. dollars (USD) annually. The infectious agent implicated in mass tilapia die-offs in two continents poses a threat to the global tilapia industry, which not only provides inexpensive dietary protein but also is a major employer in the developing world. Here we report characterization of the causative agent as a novel orthomyxo-like virus, tilapia lake virus (TiLV). We also describe complete genomic and protein sequences that will facilitate TiLV detection and containment and enable vaccine development.
format	article
author	Eran Bacharach Nischay Mishra Thomas Briese Michael C. Zody Japhette Esther Kembou Tsofack Rachel Zamostiano Asaf Berkowitz James Ng Adam Nitido André Corvelo Nora C. Toussaint Sandra Cathrine Abel Nielsen Mady Hornig Jorge Del Pozo Toby Bloom Hugh Ferguson Avi Eldar W. Ian Lipkin
author_facet	Eran Bacharach Nischay Mishra Thomas Briese Michael C. Zody Japhette Esther Kembou Tsofack Rachel Zamostiano Asaf Berkowitz James Ng Adam Nitido André Corvelo Nora C. Toussaint Sandra Cathrine Abel Nielsen Mady Hornig Jorge Del Pozo Toby Bloom Hugh Ferguson Avi Eldar W. Ian Lipkin
author_sort	Eran Bacharach
title	Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia
title_short	Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia
title_full	Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia
title_fullStr	Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia
title_full_unstemmed	Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia
title_sort	characterization of a novel orthomyxo-like virus causing mass die-offs of tilapia
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/50b88d96a40f4c0ead256d47f3512cd7
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Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia

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