Two LEAFY homologs ILFY1 and ILFY2 control reproductive and vegetative developments in Isoetes L.

Abstract LEAFY (LFY) is a plant-specific transcription factor, which is found in algae and all land plants. LFY homologs exert ancestral roles in regulating cell division and obtain novel functions to control floral identity. Isoetes L. is an ancient genus of heterosporous lycophytes. However, chara...

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Autores principales:	Tao Yang, Ming-fang Du, You-hao Guo, Xing Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/50c4ce43af4b49159de31d80b62ac0bb
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spelling	oai:doaj.org-article:50c4ce43af4b49159de31d80b62ac0bb2021-12-02T15:05:36ZTwo LEAFY homologs ILFY1 and ILFY2 control reproductive and vegetative developments in Isoetes L.10.1038/s41598-017-00297-32045-2322https://doaj.org/article/50c4ce43af4b49159de31d80b62ac0bb2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00297-3https://doaj.org/toc/2045-2322Abstract LEAFY (LFY) is a plant-specific transcription factor, which is found in algae and all land plants. LFY homologs exert ancestral roles in regulating cell division and obtain novel functions to control floral identity. Isoetes L. is an ancient genus of heterosporous lycophytes. However, characters about LFY homologs in lycophytes remain poorly investigated. In this study, two LFY homologs, ILFY1 and ILFY2, were cloned from five Isoetes species, including I. hypsophila, I. yunguiensis, I. sinensis, I. orientalis, and I. taiwanensis. The full length of ILFY1 was 1449–1456 bp with an open reading frame (ORF) of 927–936 bp. The full length of ILFY2 was 1768 bp with ORF of 726 bp. Phylogenetic tree revealed that ILFY1 and ILFY2 were separated into two clades, and I. hypsophila were separated with the others. Expression analysis demonstrated that IsLFY1 and IsLFY2 for I. sinensis did not show functional diversity. The two transcripts were similarly accumulated in both vegetative and reproductive tissues and highly expressed in juvenile tissues. In addition, the IsLFY1 and IsLFY2 transgenic Arabidopsis similarly did not promote precocious flowering, and they were inactive to rescue lfy mutants. The results facilitate general understandings about the characteristics of LFY in Isoetes and evolutionary process.Tao YangMing-fang DuYou-hao GuoXing LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tao Yang Ming-fang Du You-hao Guo Xing Liu Two LEAFY homologs ILFY1 and ILFY2 control reproductive and vegetative developments in Isoetes L.
description	Abstract LEAFY (LFY) is a plant-specific transcription factor, which is found in algae and all land plants. LFY homologs exert ancestral roles in regulating cell division and obtain novel functions to control floral identity. Isoetes L. is an ancient genus of heterosporous lycophytes. However, characters about LFY homologs in lycophytes remain poorly investigated. In this study, two LFY homologs, ILFY1 and ILFY2, were cloned from five Isoetes species, including I. hypsophila, I. yunguiensis, I. sinensis, I. orientalis, and I. taiwanensis. The full length of ILFY1 was 1449–1456 bp with an open reading frame (ORF) of 927–936 bp. The full length of ILFY2 was 1768 bp with ORF of 726 bp. Phylogenetic tree revealed that ILFY1 and ILFY2 were separated into two clades, and I. hypsophila were separated with the others. Expression analysis demonstrated that IsLFY1 and IsLFY2 for I. sinensis did not show functional diversity. The two transcripts were similarly accumulated in both vegetative and reproductive tissues and highly expressed in juvenile tissues. In addition, the IsLFY1 and IsLFY2 transgenic Arabidopsis similarly did not promote precocious flowering, and they were inactive to rescue lfy mutants. The results facilitate general understandings about the characteristics of LFY in Isoetes and evolutionary process.
format	article
author	Tao Yang Ming-fang Du You-hao Guo Xing Liu
author_facet	Tao Yang Ming-fang Du You-hao Guo Xing Liu
author_sort	Tao Yang
title	Two LEAFY homologs ILFY1 and ILFY2 control reproductive and vegetative developments in Isoetes L.
title_short	Two LEAFY homologs ILFY1 and ILFY2 control reproductive and vegetative developments in Isoetes L.
title_full	Two LEAFY homologs ILFY1 and ILFY2 control reproductive and vegetative developments in Isoetes L.
title_fullStr	Two LEAFY homologs ILFY1 and ILFY2 control reproductive and vegetative developments in Isoetes L.
title_full_unstemmed	Two LEAFY homologs ILFY1 and ILFY2 control reproductive and vegetative developments in Isoetes L.
title_sort	two leafy homologs ilfy1 and ilfy2 control reproductive and vegetative developments in isoetes l.
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/50c4ce43af4b49159de31d80b62ac0bb
work_keys_str_mv	AT taoyang twoleafyhomologsilfy1andilfy2controlreproductiveandvegetativedevelopmentsinisoetesl AT mingfangdu twoleafyhomologsilfy1andilfy2controlreproductiveandvegetativedevelopmentsinisoetesl AT youhaoguo twoleafyhomologsilfy1andilfy2controlreproductiveandvegetativedevelopmentsinisoetesl AT xingliu twoleafyhomologsilfy1andilfy2controlreproductiveandvegetativedevelopmentsinisoetesl
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Two LEAFY homologs ILFY1 and ILFY2 control reproductive and vegetative developments in Isoetes L.

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