Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics

Abstract. Rafi M, Septaningsih DA, Karomah AH, Lukman, Prajogo B, Amran MB, Rohman A. 2021. Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics. Biodivers...

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Autores principales:	Mohamad Rafi, DEWI ANGGRAINI SEPTANINGSIH, ALFI HUDATUL KAROMAH, LUKMAN LUKMAN, BAMBANG PRAJOGO, MUHAMMAD BACHRI AMRAN, ABDUL ROHMAN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	andrographis paniculata inhibition of α-glucosidase metabolomics ftir uhplc-q-orbitrap hrms Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/50c846f432754d9c8e426cf18c5c2586
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spelling	oai:doaj.org-article:50c846f432754d9c8e426cf18c5c25862021-11-22T00:56:36ZInhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics1412-033X2085-472210.13057/biodiv/d220359https://doaj.org/article/50c846f432754d9c8e426cf18c5c25862021-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7798https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Rafi M, Septaningsih DA, Karomah AH, Lukman, Prajogo B, Amran MB, Rohman A. 2021. Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics. Biodiversitas 22: 1535-1542. Ensuring consistency of quality, safety, and efficacy of herbal medicines from raw materials to finished products is important because of the variability in medicinal plants' active components. One of the plants that have been used as an antidiabetic herbal medicine is A. paniculata. This study aims to determine ?-glucosidase inhibitory activity, the content of several metals, FTIR spectrum profile, and putative identification of A. paniculata metabolites using UHPLC-Q-Orbitrap HRMS from different geographical origin. We found that ethanol extract of A. paniculata gave higher inhibition of ?-glucosidase activity compared to water extract. Eight metals were determined using FAAS and FAES, and calcium showed the highest content in all A. paniculata samples. FTIR spectra of A. paniculata showed a similar profile and only differed in the absorbance. We detected the presence of OH, C=O, C=C aromatic, and C-O functional groups in A. paniculata extract. About 32 metabolites were putatively identified in A. paniculata, mainly from phenolic and diterpene lactones class compounds based on UHPLC-Q-Orbitrap HRMS. Using a combination of FTIR spectra and peak area from 32 identified peaks, we can clustered A. paniculata based on its geographical origin. Based on the result obtained, the geographical origin of A. paniculata affected the metals and metabolites composition and level, resulting in different levels of ?-glucosidase inhibitory activity.Mohamad RafiDEWI ANGGRAINI SEPTANINGSIHALFI HUDATUL KAROMAHLUKMAN LUKMANBAMBANG PRAJOGOMUHAMMAD BACHRI AMRANABDUL ROHMANMBI & UNS Soloarticleandrographis paniculatainhibition of α-glucosidasemetabolomicsftiruhplc-q-orbitrap hrmsBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	andrographis paniculata inhibition of α-glucosidase metabolomics ftir uhplc-q-orbitrap hrms Biology (General) QH301-705.5
spellingShingle	andrographis paniculata inhibition of α-glucosidase metabolomics ftir uhplc-q-orbitrap hrms Biology (General) QH301-705.5 Mohamad Rafi DEWI ANGGRAINI SEPTANINGSIH ALFI HUDATUL KAROMAH LUKMAN LUKMAN BAMBANG PRAJOGO MUHAMMAD BACHRI AMRAN ABDUL ROHMAN Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics
description	Abstract. Rafi M, Septaningsih DA, Karomah AH, Lukman, Prajogo B, Amran MB, Rohman A. 2021. Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics. Biodiversitas 22: 1535-1542. Ensuring consistency of quality, safety, and efficacy of herbal medicines from raw materials to finished products is important because of the variability in medicinal plants' active components. One of the plants that have been used as an antidiabetic herbal medicine is A. paniculata. This study aims to determine ?-glucosidase inhibitory activity, the content of several metals, FTIR spectrum profile, and putative identification of A. paniculata metabolites using UHPLC-Q-Orbitrap HRMS from different geographical origin. We found that ethanol extract of A. paniculata gave higher inhibition of ?-glucosidase activity compared to water extract. Eight metals were determined using FAAS and FAES, and calcium showed the highest content in all A. paniculata samples. FTIR spectra of A. paniculata showed a similar profile and only differed in the absorbance. We detected the presence of OH, C=O, C=C aromatic, and C-O functional groups in A. paniculata extract. About 32 metabolites were putatively identified in A. paniculata, mainly from phenolic and diterpene lactones class compounds based on UHPLC-Q-Orbitrap HRMS. Using a combination of FTIR spectra and peak area from 32 identified peaks, we can clustered A. paniculata based on its geographical origin. Based on the result obtained, the geographical origin of A. paniculata affected the metals and metabolites composition and level, resulting in different levels of ?-glucosidase inhibitory activity.
format	article
author	Mohamad Rafi DEWI ANGGRAINI SEPTANINGSIH ALFI HUDATUL KAROMAH LUKMAN LUKMAN BAMBANG PRAJOGO MUHAMMAD BACHRI AMRAN ABDUL ROHMAN
author_facet	Mohamad Rafi DEWI ANGGRAINI SEPTANINGSIH ALFI HUDATUL KAROMAH LUKMAN LUKMAN BAMBANG PRAJOGO MUHAMMAD BACHRI AMRAN ABDUL ROHMAN
author_sort	Mohamad Rafi
title	Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics
title_short	Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics
title_full	Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics
title_fullStr	Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics
title_full_unstemmed	Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics
title_sort	inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of andrographis paniculata from different geographical origins based on ftir and uhplc-q-orbitrap hrms metabolomics
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/50c846f432754d9c8e426cf18c5c2586
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Inhibition of ?-glucosidase activity, metals content, and phytochemical profiling of Andrographis paniculata from different geographical origins based on FTIR and UHPLC-Q-Orbitrap HRMS metabolomics

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