Prognostic association of starvation-induced gene expression in head and neck cancer

Abstract Autophagy-related genes (ARGs) have been implicated in the initiation and progression of malignant tumor promotion. To investigate the dynamics of expression of genes, including ARGs, head and neck squamous cell carcinoma (HNSCC) cells were placed under serum-free conditions to induce growt...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Masakazu Hamada, Hiroaki Inaba, Kyoko Nishiyama, Sho Yoshida, Yoshiaki Yura, Michiyo Matsumoto-Nakano, Narikazu Uzawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/50ccf741aa1c4cac93bd87c7a353cb38
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:50ccf741aa1c4cac93bd87c7a353cb38
record_format	dspace
spelling	oai:doaj.org-article:50ccf741aa1c4cac93bd87c7a353cb382021-12-02T17:17:39ZPrognostic association of starvation-induced gene expression in head and neck cancer10.1038/s41598-021-98544-12045-2322https://doaj.org/article/50ccf741aa1c4cac93bd87c7a353cb382021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98544-1https://doaj.org/toc/2045-2322Abstract Autophagy-related genes (ARGs) have been implicated in the initiation and progression of malignant tumor promotion. To investigate the dynamics of expression of genes, including ARGs, head and neck squamous cell carcinoma (HNSCC) cells were placed under serum-free conditions to induce growth retardation and autophagy, and these starved cells were subjected to transcriptome analysis. Among the 21 starvation-induced genes (SIGs) located in the autophagy, cell proliferation, and survival signaling pathways, we identified SIGs that showed prominent up-regulation or down-regulation in vitro. These included AGR2, BST2, CALR, CD22, DDIT3, FOXA2, HSPA5, PIWIL4, PYCR1, SGK3, and TRIB3. The Cancer Genome Atlas (TCGA) database of HNSCC patients was used to examine the expression of up-regulated genes, and CALR, HSPA5, and TRIB3 were found to be highly expressed relative to solid normal tissue in cancer and the survival rate was reduced in patients with high expression. Protein–protein interaction analysis demonstrated the formation of a dense network of these genes. Cox regression analysis revealed that high expression of CALR, HSPA5, and TRIB3 was associated with poor prognosis in patients with TCGA-HNSCC. Therefore, these SIGs up-regulated under serum starvation may be molecular prognostic markers in HNSCC patients.Masakazu HamadaHiroaki InabaKyoko NishiyamaSho YoshidaYoshiaki YuraMichiyo Matsumoto-NakanoNarikazu UzawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masakazu Hamada Hiroaki Inaba Kyoko Nishiyama Sho Yoshida Yoshiaki Yura Michiyo Matsumoto-Nakano Narikazu Uzawa Prognostic association of starvation-induced gene expression in head and neck cancer
description	Abstract Autophagy-related genes (ARGs) have been implicated in the initiation and progression of malignant tumor promotion. To investigate the dynamics of expression of genes, including ARGs, head and neck squamous cell carcinoma (HNSCC) cells were placed under serum-free conditions to induce growth retardation and autophagy, and these starved cells were subjected to transcriptome analysis. Among the 21 starvation-induced genes (SIGs) located in the autophagy, cell proliferation, and survival signaling pathways, we identified SIGs that showed prominent up-regulation or down-regulation in vitro. These included AGR2, BST2, CALR, CD22, DDIT3, FOXA2, HSPA5, PIWIL4, PYCR1, SGK3, and TRIB3. The Cancer Genome Atlas (TCGA) database of HNSCC patients was used to examine the expression of up-regulated genes, and CALR, HSPA5, and TRIB3 were found to be highly expressed relative to solid normal tissue in cancer and the survival rate was reduced in patients with high expression. Protein–protein interaction analysis demonstrated the formation of a dense network of these genes. Cox regression analysis revealed that high expression of CALR, HSPA5, and TRIB3 was associated with poor prognosis in patients with TCGA-HNSCC. Therefore, these SIGs up-regulated under serum starvation may be molecular prognostic markers in HNSCC patients.
format	article
author	Masakazu Hamada Hiroaki Inaba Kyoko Nishiyama Sho Yoshida Yoshiaki Yura Michiyo Matsumoto-Nakano Narikazu Uzawa
author_facet	Masakazu Hamada Hiroaki Inaba Kyoko Nishiyama Sho Yoshida Yoshiaki Yura Michiyo Matsumoto-Nakano Narikazu Uzawa
author_sort	Masakazu Hamada
title	Prognostic association of starvation-induced gene expression in head and neck cancer
title_short	Prognostic association of starvation-induced gene expression in head and neck cancer
title_full	Prognostic association of starvation-induced gene expression in head and neck cancer
title_fullStr	Prognostic association of starvation-induced gene expression in head and neck cancer
title_full_unstemmed	Prognostic association of starvation-induced gene expression in head and neck cancer
title_sort	prognostic association of starvation-induced gene expression in head and neck cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/50ccf741aa1c4cac93bd87c7a353cb38
work_keys_str_mv	AT masakazuhamada prognosticassociationofstarvationinducedgeneexpressioninheadandneckcancer AT hiroakiinaba prognosticassociationofstarvationinducedgeneexpressioninheadandneckcancer AT kyokonishiyama prognosticassociationofstarvationinducedgeneexpressioninheadandneckcancer AT shoyoshida prognosticassociationofstarvationinducedgeneexpressioninheadandneckcancer AT yoshiakiyura prognosticassociationofstarvationinducedgeneexpressioninheadandneckcancer AT michiyomatsumotonakano prognosticassociationofstarvationinducedgeneexpressioninheadandneckcancer AT narikazuuzawa prognosticassociationofstarvationinducedgeneexpressioninheadandneckcancer
_version_	1718381143949049856

Prognostic association of starvation-induced gene expression in head and neck cancer

Ejemplares similares