Early inflammatory events of mastitis—a pilot study with the isolated perfused bovine udder

Abstract Background Bovine mastitis is an important health and cost factor in the milk industry. To elucidate whether isolated perfused bovine udders can be used to study early inflammatory events of mastitis, 1 mg of lipopolysaccharide (LPS) was instilled into quarters of 10 isolated perfused bovin...

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Autores principales:	Kathrin Susanne Brand, Viviane Filor, Wolfgang Bäumer
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Isolated perfused bovine udder Mastitis Lipopolysaccharide Inflammation Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/50d19a7fbce346b59de456f55e726e93
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spelling	oai:doaj.org-article:50d19a7fbce346b59de456f55e726e932021-11-21T12:25:58ZEarly inflammatory events of mastitis—a pilot study with the isolated perfused bovine udder10.1186/s12917-021-03029-y1746-6148https://doaj.org/article/50d19a7fbce346b59de456f55e726e932021-11-01T00:00:00Zhttps://doi.org/10.1186/s12917-021-03029-yhttps://doaj.org/toc/1746-6148Abstract Background Bovine mastitis is an important health and cost factor in the milk industry. To elucidate whether isolated perfused bovine udders can be used to study early inflammatory events of mastitis, 1 mg of lipopolysaccharide (LPS) was instilled into quarters of 10 isolated perfused bovine udders. Three hours and 6 h after LPS instillation, tissue samples were taken from the gland cistern and base of the udder, subsequently stored in RNAlater and processed for the determination of inflammation-dependent gene regulation by real-time RT-qPCR. Gene expression analysis was performed using delta-delta Ct method. To translate mRNA results to protein, IL-1ß and IL-6 were determined in tissue homogenate by ELISA. Results The instillation of 1 mg LPS lead to an increased expression of pro-inflammatory cytokines and chemokines like TNF-α, CCL20, CXCL8 as well as of IL-1 ß, IL-6 and IL-10, lingual antimicrobial peptide (LAP) and S100A9. However, the degree of elevation differed slightly between gland cistern and udder base and markedly between 3 and 6 h after instillation, with a distinct increase in mediator expression after 6 h. IL-1β protein increased in a time-dependent manner, whereas IL-6 was unchanged within 6 h of LPS instillation. Conclusion Compared to in vivo studies with instillation of LPS into udders of living cows, a similar inflammation-dependent gene regulation profile can be mimicked in the isolated perfused bovine udder, indicating a supplementation of animal experiments. Graphical abstractKathrin Susanne BrandViviane FilorWolfgang BäumerBMCarticleIsolated perfused bovine udderMastitisLipopolysaccharideInflammationVeterinary medicineSF600-1100ENBMC Veterinary Research, Vol 17, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Isolated perfused bovine udder Mastitis Lipopolysaccharide Inflammation Veterinary medicine SF600-1100
spellingShingle	Isolated perfused bovine udder Mastitis Lipopolysaccharide Inflammation Veterinary medicine SF600-1100 Kathrin Susanne Brand Viviane Filor Wolfgang Bäumer Early inflammatory events of mastitis—a pilot study with the isolated perfused bovine udder
description	Abstract Background Bovine mastitis is an important health and cost factor in the milk industry. To elucidate whether isolated perfused bovine udders can be used to study early inflammatory events of mastitis, 1 mg of lipopolysaccharide (LPS) was instilled into quarters of 10 isolated perfused bovine udders. Three hours and 6 h after LPS instillation, tissue samples were taken from the gland cistern and base of the udder, subsequently stored in RNAlater and processed for the determination of inflammation-dependent gene regulation by real-time RT-qPCR. Gene expression analysis was performed using delta-delta Ct method. To translate mRNA results to protein, IL-1ß and IL-6 were determined in tissue homogenate by ELISA. Results The instillation of 1 mg LPS lead to an increased expression of pro-inflammatory cytokines and chemokines like TNF-α, CCL20, CXCL8 as well as of IL-1 ß, IL-6 and IL-10, lingual antimicrobial peptide (LAP) and S100A9. However, the degree of elevation differed slightly between gland cistern and udder base and markedly between 3 and 6 h after instillation, with a distinct increase in mediator expression after 6 h. IL-1β protein increased in a time-dependent manner, whereas IL-6 was unchanged within 6 h of LPS instillation. Conclusion Compared to in vivo studies with instillation of LPS into udders of living cows, a similar inflammation-dependent gene regulation profile can be mimicked in the isolated perfused bovine udder, indicating a supplementation of animal experiments. Graphical abstract
format	article
author	Kathrin Susanne Brand Viviane Filor Wolfgang Bäumer
author_facet	Kathrin Susanne Brand Viviane Filor Wolfgang Bäumer
author_sort	Kathrin Susanne Brand
title	Early inflammatory events of mastitis—a pilot study with the isolated perfused bovine udder
title_short	Early inflammatory events of mastitis—a pilot study with the isolated perfused bovine udder
title_full	Early inflammatory events of mastitis—a pilot study with the isolated perfused bovine udder
title_fullStr	Early inflammatory events of mastitis—a pilot study with the isolated perfused bovine udder
title_full_unstemmed	Early inflammatory events of mastitis—a pilot study with the isolated perfused bovine udder
title_sort	early inflammatory events of mastitis—a pilot study with the isolated perfused bovine udder
publisher	BMC
publishDate	2021
url	https://doaj.org/article/50d19a7fbce346b59de456f55e726e93
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Early inflammatory events of mastitis—a pilot study with the isolated perfused bovine udder

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