Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis

Label-free SERS is a powerful bio-analytical technique in which molecular fingerprinting is combined with localized surface plasmons (LSPs) on metal surfaces to achieve high sensitivity. Silver and gold colloids are among the most common nanostructured substrates used in SERS, but since protein-rich...

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Autores principales:	Alessandro Esposito, Alois Bonifacio, Valter Sergo, Stefano Fornasaro
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	label-free SERS biofluids plasmonic paper vibrational spectroscopy serum Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/50d1b2237e58452a80c403c4b8ada0b3
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id	oai:doaj.org-article:50d1b2237e58452a80c403c4b8ada0b3
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spelling	oai:doaj.org-article:50d1b2237e58452a80c403c4b8ada0b32021-11-25T16:55:52ZLabel-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis10.3390/bios111104672079-6374https://doaj.org/article/50d1b2237e58452a80c403c4b8ada0b32021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/467https://doaj.org/toc/2079-6374Label-free SERS is a powerful bio-analytical technique in which molecular fingerprinting is combined with localized surface plasmons (LSPs) on metal surfaces to achieve high sensitivity. Silver and gold colloids are among the most common nanostructured substrates used in SERS, but since protein-rich samples such as serum or plasma can hinder the SERS effect due to protein–substrate interactions, they often require a deproteinization step. Moreover, SERS methods based on metal colloids often suffer from a poor reproducibility. Here, we propose a paper-based SERS sampling method in which unprocessed human serum samples are first soaked on paper strips (0.4 × 2 cm<sup>2</sup>), and then mixed with colloidal silver nanoparticles by centrifugation to obtain a Centrifugal Silver Plasmonic Paper (CSPP). The CSPP methodology has the potential to become a promising tool in bioanalytical SERS applications: it uses common colloidal substrates but without the need for sample deproteinization, while having a good reproducibility both in terms of overall spectral shape (r > 0.96) and absolute intensity (RSD < 10%). Moreover, this methodology allows SERS analysis more than one month after serum collection on the paper strip, facilitating storage and handling of clinical samples (including shipping from clinical sites to labs).Alessandro EspositoAlois BonifacioValter SergoStefano FornasaroMDPI AGarticlelabel-free SERSbiofluidsplasmonic papervibrational spectroscopyserumBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 467, p 467 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	label-free SERS biofluids plasmonic paper vibrational spectroscopy serum Biotechnology TP248.13-248.65
spellingShingle	label-free SERS biofluids plasmonic paper vibrational spectroscopy serum Biotechnology TP248.13-248.65 Alessandro Esposito Alois Bonifacio Valter Sergo Stefano Fornasaro Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis
description	Label-free SERS is a powerful bio-analytical technique in which molecular fingerprinting is combined with localized surface plasmons (LSPs) on metal surfaces to achieve high sensitivity. Silver and gold colloids are among the most common nanostructured substrates used in SERS, but since protein-rich samples such as serum or plasma can hinder the SERS effect due to protein–substrate interactions, they often require a deproteinization step. Moreover, SERS methods based on metal colloids often suffer from a poor reproducibility. Here, we propose a paper-based SERS sampling method in which unprocessed human serum samples are first soaked on paper strips (0.4 × 2 cm<sup>2</sup>), and then mixed with colloidal silver nanoparticles by centrifugation to obtain a Centrifugal Silver Plasmonic Paper (CSPP). The CSPP methodology has the potential to become a promising tool in bioanalytical SERS applications: it uses common colloidal substrates but without the need for sample deproteinization, while having a good reproducibility both in terms of overall spectral shape (r > 0.96) and absolute intensity (RSD < 10%). Moreover, this methodology allows SERS analysis more than one month after serum collection on the paper strip, facilitating storage and handling of clinical samples (including shipping from clinical sites to labs).
format	article
author	Alessandro Esposito Alois Bonifacio Valter Sergo Stefano Fornasaro
author_facet	Alessandro Esposito Alois Bonifacio Valter Sergo Stefano Fornasaro
author_sort	Alessandro Esposito
title	Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis
title_short	Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis
title_full	Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis
title_fullStr	Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis
title_full_unstemmed	Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis
title_sort	label-free surface enhanced raman scattering (sers) on centrifugal silver plasmonic paper (cspp): a novel methodology for unprocessed biofluids sampling and analysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/50d1b2237e58452a80c403c4b8ada0b3
work_keys_str_mv	AT alessandroesposito labelfreesurfaceenhancedramanscatteringsersoncentrifugalsilverplasmonicpapercsppanovelmethodologyforunprocessedbiofluidssamplingandanalysis AT aloisbonifacio labelfreesurfaceenhancedramanscatteringsersoncentrifugalsilverplasmonicpapercsppanovelmethodologyforunprocessedbiofluidssamplingandanalysis AT valtersergo labelfreesurfaceenhancedramanscatteringsersoncentrifugalsilverplasmonicpapercsppanovelmethodologyforunprocessedbiofluidssamplingandanalysis AT stefanofornasaro labelfreesurfaceenhancedramanscatteringsersoncentrifugalsilverplasmonicpapercsppanovelmethodologyforunprocessedbiofluidssamplingandanalysis
_version_	1718412836058693632

Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis

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