Caffeic Acid Attenuates Diabetic Kidney Disease via Modulation of Autophagy in a High-Fat Diet/Streptozotocin- Induced Diabetic Rat

Abstract The aim of this study is to evaluate the anti-diabetic nephropathy effect of Caffeic acid and to prove our hypothesis for its mechanism of action that it may occur by reactivation of autophagy pathway via suppression of autophagy regulatory miRNAs. In vivo, high-fat diet and streptozotocin-...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marwa Matboli, Sanaa Eissa, Doaa Ibrahim, Marwa G. A. Hegazy, Shalabia S. Imam, Eman K. Habib
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/50d37152bb5e4fe28082f840852bbfb3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:50d37152bb5e4fe28082f840852bbfb3
record_format	dspace
spelling	oai:doaj.org-article:50d37152bb5e4fe28082f840852bbfb32021-12-02T12:30:54ZCaffeic Acid Attenuates Diabetic Kidney Disease via Modulation of Autophagy in a High-Fat Diet/Streptozotocin- Induced Diabetic Rat10.1038/s41598-017-02320-z2045-2322https://doaj.org/article/50d37152bb5e4fe28082f840852bbfb32017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02320-zhttps://doaj.org/toc/2045-2322Abstract The aim of this study is to evaluate the anti-diabetic nephropathy effect of Caffeic acid and to prove our hypothesis for its mechanism of action that it may occur by reactivation of autophagy pathway via suppression of autophagy regulatory miRNAs. In vivo, high-fat diet and streptozotocin-induced (HFD-STZ) diabetic rats were treated with Caffeic acid once per day for 12 weeks before and after development of diabetic nephropathy. Blood and urine biochemical parameters, autophagy transcripts and their epigenetic regulators together with renal tissue morphology were investigated. In diabetic rats, Caffeic acid intake, caused improvement in albumin excretion,blood glucose, reduced renal mesangial matrix extension with increased vacuolation and reappearance of autophagosomes. Meanwhile, it resulted in autophagy genes up-regulation [RB 1-inducible coiled coil protein (RB1CC1), Microtubule-associated proteins 1A/1B light chain 3(MAP1LC3B), Autophagy related gene (ATG-12),] with simultaneous reduction in their epigenetic regulators; miRNA-133b, −342 and 30a, respectively. These above mentioned effects were more significant in the diabetic nephropathy Caffeic treated rats than in the prophylactic group. Based on our results we postulated that caffeic acid modulates autophagy pathway through inhibition of autophagy regulatory miRNAs, that could explain its curative properties against diabetic kidney disease.Marwa MatboliSanaa EissaDoaa IbrahimMarwa G. A. HegazyShalabia S. ImamEman K. HabibNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marwa Matboli Sanaa Eissa Doaa Ibrahim Marwa G. A. Hegazy Shalabia S. Imam Eman K. Habib Caffeic Acid Attenuates Diabetic Kidney Disease via Modulation of Autophagy in a High-Fat Diet/Streptozotocin- Induced Diabetic Rat
description	Abstract The aim of this study is to evaluate the anti-diabetic nephropathy effect of Caffeic acid and to prove our hypothesis for its mechanism of action that it may occur by reactivation of autophagy pathway via suppression of autophagy regulatory miRNAs. In vivo, high-fat diet and streptozotocin-induced (HFD-STZ) diabetic rats were treated with Caffeic acid once per day for 12 weeks before and after development of diabetic nephropathy. Blood and urine biochemical parameters, autophagy transcripts and their epigenetic regulators together with renal tissue morphology were investigated. In diabetic rats, Caffeic acid intake, caused improvement in albumin excretion,blood glucose, reduced renal mesangial matrix extension with increased vacuolation and reappearance of autophagosomes. Meanwhile, it resulted in autophagy genes up-regulation [RB 1-inducible coiled coil protein (RB1CC1), Microtubule-associated proteins 1A/1B light chain 3(MAP1LC3B), Autophagy related gene (ATG-12),] with simultaneous reduction in their epigenetic regulators; miRNA-133b, −342 and 30a, respectively. These above mentioned effects were more significant in the diabetic nephropathy Caffeic treated rats than in the prophylactic group. Based on our results we postulated that caffeic acid modulates autophagy pathway through inhibition of autophagy regulatory miRNAs, that could explain its curative properties against diabetic kidney disease.
format	article
author	Marwa Matboli Sanaa Eissa Doaa Ibrahim Marwa G. A. Hegazy Shalabia S. Imam Eman K. Habib
author_facet	Marwa Matboli Sanaa Eissa Doaa Ibrahim Marwa G. A. Hegazy Shalabia S. Imam Eman K. Habib
author_sort	Marwa Matboli
title	Caffeic Acid Attenuates Diabetic Kidney Disease via Modulation of Autophagy in a High-Fat Diet/Streptozotocin- Induced Diabetic Rat
title_short	Caffeic Acid Attenuates Diabetic Kidney Disease via Modulation of Autophagy in a High-Fat Diet/Streptozotocin- Induced Diabetic Rat
title_full	Caffeic Acid Attenuates Diabetic Kidney Disease via Modulation of Autophagy in a High-Fat Diet/Streptozotocin- Induced Diabetic Rat
title_fullStr	Caffeic Acid Attenuates Diabetic Kidney Disease via Modulation of Autophagy in a High-Fat Diet/Streptozotocin- Induced Diabetic Rat
title_full_unstemmed	Caffeic Acid Attenuates Diabetic Kidney Disease via Modulation of Autophagy in a High-Fat Diet/Streptozotocin- Induced Diabetic Rat
title_sort	caffeic acid attenuates diabetic kidney disease via modulation of autophagy in a high-fat diet/streptozotocin- induced diabetic rat
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/50d37152bb5e4fe28082f840852bbfb3
work_keys_str_mv	AT marwamatboli caffeicacidattenuatesdiabetickidneydiseaseviamodulationofautophagyinahighfatdietstreptozotocininduceddiabeticrat AT sanaaeissa caffeicacidattenuatesdiabetickidneydiseaseviamodulationofautophagyinahighfatdietstreptozotocininduceddiabeticrat AT doaaibrahim caffeicacidattenuatesdiabetickidneydiseaseviamodulationofautophagyinahighfatdietstreptozotocininduceddiabeticrat AT marwagahegazy caffeicacidattenuatesdiabetickidneydiseaseviamodulationofautophagyinahighfatdietstreptozotocininduceddiabeticrat AT shalabiasimam caffeicacidattenuatesdiabetickidneydiseaseviamodulationofautophagyinahighfatdietstreptozotocininduceddiabeticrat AT emankhabib caffeicacidattenuatesdiabetickidneydiseaseviamodulationofautophagyinahighfatdietstreptozotocininduceddiabeticrat
_version_	1718394277675925504

Caffeic Acid Attenuates Diabetic Kidney Disease via Modulation of Autophagy in a High-Fat Diet/Streptozotocin- Induced Diabetic Rat

Ejemplares similares