Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy

Abstract The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of h...

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Autores principales:	Tibor Z. Veres, Tamás Kopcsányi, Marko Tirri, Armin Braun, Masayuki Miyasaka, Ronald N. Germain, Sirpa Jalkanen, Marko Salmi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/50d69311e3a24a059be6d61846273554
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spelling	oai:doaj.org-article:50d69311e3a24a059be6d618462735542021-12-02T15:05:33ZIntubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy10.1038/s41598-017-00769-62045-2322https://doaj.org/article/50d69311e3a24a059be6d618462735542017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00769-6https://doaj.org/toc/2045-2322Abstract The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.Tibor Z. VeresTamás KopcsányiMarko TirriArmin BraunMasayuki MiyasakaRonald N. GermainSirpa JalkanenMarko SalmiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tibor Z. Veres Tamás Kopcsányi Marko Tirri Armin Braun Masayuki Miyasaka Ronald N. Germain Sirpa Jalkanen Marko Salmi Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
description	Abstract The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.
format	article
author	Tibor Z. Veres Tamás Kopcsányi Marko Tirri Armin Braun Masayuki Miyasaka Ronald N. Germain Sirpa Jalkanen Marko Salmi
author_facet	Tibor Z. Veres Tamás Kopcsányi Marko Tirri Armin Braun Masayuki Miyasaka Ronald N. Germain Sirpa Jalkanen Marko Salmi
author_sort	Tibor Z. Veres
title	Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_short	Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_full	Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_fullStr	Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_full_unstemmed	Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_sort	intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/50d69311e3a24a059be6d61846273554
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Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy

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