Ocular hypotensive effects of a Rho-associated protein kinase inhibitor in rabbits

Muhammad Irfan Kamaruddin,1,2 Momoko Nakamura-Shibasaki,1 Yu Mizuno,1 Yoshiaki Kiuchi1 1Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan; 2Department of Ophthalmology, Hasanuddin University, Makassar, South Sulawesi, Ind...

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Autores principales:	Kamaruddin MI, Nakamura-Shibasaki M, Mizuno Y, Kiuchi Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	ripasudil timolol pilocarpine dorzolamide intraocular pressure rabbit Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/50d8dcb4a78547c7bca71e46c83a9138
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spelling	oai:doaj.org-article:50d8dcb4a78547c7bca71e46c83a91382021-12-02T07:35:31ZOcular hypotensive effects of a Rho-associated protein kinase inhibitor in rabbits1177-5483https://doaj.org/article/50d8dcb4a78547c7bca71e46c83a91382017-03-01T00:00:00Zhttps://www.dovepress.com/ocular-hypotensive-effects-of-a-rho-associated-protein-kinase-inhibito-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Muhammad Irfan Kamaruddin,1,2 Momoko Nakamura-Shibasaki,1 Yu Mizuno,1 Yoshiaki Kiuchi1 1Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan; 2Department of Ophthalmology, Hasanuddin University, Makassar, South Sulawesi, Indonesia Purpose: Ripasudil is a novel Rho-associated protein kinase inhibitor that is used to treat ocular hypertension. However, the comparison of the intraocular pressure (IOP)-lowering effects between ripasudil alone and other ocular hypotensive drugs has not been studied thoroughly. The purpose of this study is to examine the ocular hypotensive effects of 0.4% ripasudil, 2% pilocarpine, 0.5% timolol and 0.1% dorzolamide in rabbits. We also studied the IOP changes when 0.4% ripasudil was combined with 2% pilocarpine, 0.5% timolol or 0.1% dorzolamide. Methods: One drop of saline solution, 0.4% ripasudil, 0.5% timolol, 2% pilocarpine or 1% dorzolamide or a combination of these agents was applied topically to the left eyes of eight healthy albino rabbits. Posttreatment changes in the IOP of albino rabbits were monitored using a rebound tonometer over a 5-h time course. Changes in IOP after application of saline served as the control. One-way analysis of variance and Dunnett’s post hoc tests were used for statistical analyses. Results: After topical instillation, 0.4% ripasudil resulted in significant decreases in IOP at 0.5 and 1 h compared with the control group. Treatment with timolol, pilocarpine or dorzolamide had no significant effect on IOP. Treatment with timolol, pilocarpine or dorzolamide in combination with ripasudil resulted in significant reductions in IOP at 1 h. However, none of these agents enhanced the IOP-lowering effects of ripasudil. Conclusion: Ripasudil has stronger IOP-lowering effects than timolol, pilocarpine or dorzolamide hypotensive agents in our rabbit model. Addition of timolol, pilocarpine or dorzolamide did not enhance the IOP-lowering effects of ripasudil alone. Keywords: ripasudil, timolol, pilocarpine, dorzolamide, intraocular pressure, rabbitKamaruddin MINakamura-Shibasaki MMizuno YKiuchi YDove Medical Pressarticleripasudiltimololpilocarpinedorzolamideintraocular pressurerabbitOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 11, Pp 591-597 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	ripasudil timolol pilocarpine dorzolamide intraocular pressure rabbit Ophthalmology RE1-994
spellingShingle	ripasudil timolol pilocarpine dorzolamide intraocular pressure rabbit Ophthalmology RE1-994 Kamaruddin MI Nakamura-Shibasaki M Mizuno Y Kiuchi Y Ocular hypotensive effects of a Rho-associated protein kinase inhibitor in rabbits
description	Muhammad Irfan Kamaruddin,1,2 Momoko Nakamura-Shibasaki,1 Yu Mizuno,1 Yoshiaki Kiuchi1 1Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan; 2Department of Ophthalmology, Hasanuddin University, Makassar, South Sulawesi, Indonesia Purpose: Ripasudil is a novel Rho-associated protein kinase inhibitor that is used to treat ocular hypertension. However, the comparison of the intraocular pressure (IOP)-lowering effects between ripasudil alone and other ocular hypotensive drugs has not been studied thoroughly. The purpose of this study is to examine the ocular hypotensive effects of 0.4% ripasudil, 2% pilocarpine, 0.5% timolol and 0.1% dorzolamide in rabbits. We also studied the IOP changes when 0.4% ripasudil was combined with 2% pilocarpine, 0.5% timolol or 0.1% dorzolamide. Methods: One drop of saline solution, 0.4% ripasudil, 0.5% timolol, 2% pilocarpine or 1% dorzolamide or a combination of these agents was applied topically to the left eyes of eight healthy albino rabbits. Posttreatment changes in the IOP of albino rabbits were monitored using a rebound tonometer over a 5-h time course. Changes in IOP after application of saline served as the control. One-way analysis of variance and Dunnett’s post hoc tests were used for statistical analyses. Results: After topical instillation, 0.4% ripasudil resulted in significant decreases in IOP at 0.5 and 1 h compared with the control group. Treatment with timolol, pilocarpine or dorzolamide had no significant effect on IOP. Treatment with timolol, pilocarpine or dorzolamide in combination with ripasudil resulted in significant reductions in IOP at 1 h. However, none of these agents enhanced the IOP-lowering effects of ripasudil. Conclusion: Ripasudil has stronger IOP-lowering effects than timolol, pilocarpine or dorzolamide hypotensive agents in our rabbit model. Addition of timolol, pilocarpine or dorzolamide did not enhance the IOP-lowering effects of ripasudil alone. Keywords: ripasudil, timolol, pilocarpine, dorzolamide, intraocular pressure, rabbit
format	article
author	Kamaruddin MI Nakamura-Shibasaki M Mizuno Y Kiuchi Y
author_facet	Kamaruddin MI Nakamura-Shibasaki M Mizuno Y Kiuchi Y
author_sort	Kamaruddin MI
title	Ocular hypotensive effects of a Rho-associated protein kinase inhibitor in rabbits
title_short	Ocular hypotensive effects of a Rho-associated protein kinase inhibitor in rabbits
title_full	Ocular hypotensive effects of a Rho-associated protein kinase inhibitor in rabbits
title_fullStr	Ocular hypotensive effects of a Rho-associated protein kinase inhibitor in rabbits
title_full_unstemmed	Ocular hypotensive effects of a Rho-associated protein kinase inhibitor in rabbits
title_sort	ocular hypotensive effects of a rho-associated protein kinase inhibitor in rabbits
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/50d8dcb4a78547c7bca71e46c83a9138
work_keys_str_mv	AT kamaruddinmi ocularhypotensiveeffectsofarhoassociatedproteinkinaseinhibitorinrabbits AT nakamurashibasakim ocularhypotensiveeffectsofarhoassociatedproteinkinaseinhibitorinrabbits AT mizunoy ocularhypotensiveeffectsofarhoassociatedproteinkinaseinhibitorinrabbits AT kiuchiy ocularhypotensiveeffectsofarhoassociatedproteinkinaseinhibitorinrabbits
_version_	1718399335600750592

Ocular hypotensive effects of a Rho-associated protein kinase inhibitor in rabbits

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