Morpho‐metabotyping the oxidative stress response

Abstract Oxidative stress and reactive oxygen species (ROS) are central to many physiological and pathophysiological processes. However, due to multiple technical challenges, it is hard to capture a comprehensive readout of the cell, involving both biochemical and functional status. We addressed thi...

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Autores principales:	Mate Rusz, Giorgia Del Favero, Yasin El Abiead, Christopher Gerner, Bernhard K. Keppler, Michael A. Jakupec, Gunda Koellensperger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/50dbdd09112b498cbe5c3a1330ed7682
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spelling	oai:doaj.org-article:50dbdd09112b498cbe5c3a1330ed76822021-12-02T18:47:08ZMorpho‐metabotyping the oxidative stress response10.1038/s41598-021-94585-82045-2322https://doaj.org/article/50dbdd09112b498cbe5c3a1330ed76822021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94585-8https://doaj.org/toc/2045-2322Abstract Oxidative stress and reactive oxygen species (ROS) are central to many physiological and pathophysiological processes. However, due to multiple technical challenges, it is hard to capture a comprehensive readout of the cell, involving both biochemical and functional status. We addressed this problem by developing a fully parallelized workflow for metabolomics (providing absolute quantities for > 100 metabolites including TCA cycle, pentose phosphate pathway, purine metabolism, glutathione metabolism, cysteine and methionine metabolism, glycolysis and gluconeogenesis) and live cell imaging microscopy. The correlative imaging strategy was applied to study morphological and metabolic adaptation of cancer cells upon short-term hydrogen peroxide (H2O2) exposure in vitro. The combination provided rich metabolic information at the endpoint of exposure together with imaging of mitochondrial effects. As a response, superoxide concentrations were elevated with a strong mitochondrial localization, and multi-parametric image analysis revealed a shift towards fragmentation. In line with this, metabolism reflected both the impaired mitochondrial function and shifts to support the first-line cellular defense and compensate for energy loss. The presented workflow combining high-end technologies demonstrates the applicability for the study of short-term oxidative stress, but it can be suitable for the in-depth study of various short-term oxidative and other cellular stress-related phenomena.Mate RuszGiorgia Del FaveroYasin El AbieadChristopher GernerBernhard K. KepplerMichael A. JakupecGunda KoellenspergerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mate Rusz Giorgia Del Favero Yasin El Abiead Christopher Gerner Bernhard K. Keppler Michael A. Jakupec Gunda Koellensperger Morpho‐metabotyping the oxidative stress response
description	Abstract Oxidative stress and reactive oxygen species (ROS) are central to many physiological and pathophysiological processes. However, due to multiple technical challenges, it is hard to capture a comprehensive readout of the cell, involving both biochemical and functional status. We addressed this problem by developing a fully parallelized workflow for metabolomics (providing absolute quantities for > 100 metabolites including TCA cycle, pentose phosphate pathway, purine metabolism, glutathione metabolism, cysteine and methionine metabolism, glycolysis and gluconeogenesis) and live cell imaging microscopy. The correlative imaging strategy was applied to study morphological and metabolic adaptation of cancer cells upon short-term hydrogen peroxide (H2O2) exposure in vitro. The combination provided rich metabolic information at the endpoint of exposure together with imaging of mitochondrial effects. As a response, superoxide concentrations were elevated with a strong mitochondrial localization, and multi-parametric image analysis revealed a shift towards fragmentation. In line with this, metabolism reflected both the impaired mitochondrial function and shifts to support the first-line cellular defense and compensate for energy loss. The presented workflow combining high-end technologies demonstrates the applicability for the study of short-term oxidative stress, but it can be suitable for the in-depth study of various short-term oxidative and other cellular stress-related phenomena.
format	article
author	Mate Rusz Giorgia Del Favero Yasin El Abiead Christopher Gerner Bernhard K. Keppler Michael A. Jakupec Gunda Koellensperger
author_facet	Mate Rusz Giorgia Del Favero Yasin El Abiead Christopher Gerner Bernhard K. Keppler Michael A. Jakupec Gunda Koellensperger
author_sort	Mate Rusz
title	Morpho‐metabotyping the oxidative stress response
title_short	Morpho‐metabotyping the oxidative stress response
title_full	Morpho‐metabotyping the oxidative stress response
title_fullStr	Morpho‐metabotyping the oxidative stress response
title_full_unstemmed	Morpho‐metabotyping the oxidative stress response
title_sort	morpho‐metabotyping the oxidative stress response
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/50dbdd09112b498cbe5c3a1330ed7682
work_keys_str_mv	AT materusz morphometabotypingtheoxidativestressresponse AT giorgiadelfavero morphometabotypingtheoxidativestressresponse AT yasinelabiead morphometabotypingtheoxidativestressresponse AT christophergerner morphometabotypingtheoxidativestressresponse AT bernhardkkeppler morphometabotypingtheoxidativestressresponse AT michaelajakupec morphometabotypingtheoxidativestressresponse AT gundakoellensperger morphometabotypingtheoxidativestressresponse
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Morpho‐metabotyping the oxidative stress response

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