Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies

The parasitoid wasp Muscidifurax raptorellus (Hymenoptera: Pteromalidae) is a gregarious species that has received extensive attention for its potential in biological pest control against house fly, stable fly, and other filth flies. It has a high reproductive capacity and can be reared easily. Howe...

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Autores principales:	Xiao Xiong, Yogeshwar D Kelkar, Chris J Geden, Chao Zhang, Yidong Wang, Evelien Jongepier, Ellen O. Martinson, Eveline C Verhulst, Jürgen Gadau, John H Werren, Xu Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Muscidifurax parasitoid wasp biological control housefly linked-read technology PacBio sequencing Genetics QH426-470
Acceso en línea:	https://doaj.org/article/50e1c8c840dc46f485c328405aabb44a
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spelling	oai:doaj.org-article:50e1c8c840dc46f485c328405aabb44a2021-11-12T10:11:10ZLong-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies1664-802110.3389/fgene.2021.748135https://doaj.org/article/50e1c8c840dc46f485c328405aabb44a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.748135/fullhttps://doaj.org/toc/1664-8021The parasitoid wasp Muscidifurax raptorellus (Hymenoptera: Pteromalidae) is a gregarious species that has received extensive attention for its potential in biological pest control against house fly, stable fly, and other filth flies. It has a high reproductive capacity and can be reared easily. However, genome assembly is not available for M. raptorellus or any other species in this genus. Previously, we assembled a complete circular mitochondrial genome with a length of 24,717 bp. Here, we assembled and annotated a high-quality nuclear genome of M. raptorellus, using a combination of long-read (104× genome coverage) and short-read (326× genome coverage) sequencing technologies. The assembled genome size is 314 Mbp in 226 contigs, with a 97.9% BUSCO completeness score and a contig N50 of 4.67 Mb, suggesting excellent continuity of this assembly. Our assembly builds the foundation for comparative and evolutionary genomic analysis in the genus of Muscidifurax and possible future biocontrol applications.Xiao XiongXiao XiongYogeshwar D KelkarChris J GedenChao ZhangYidong WangEvelien JongepierEllen O. MartinsonEllen O. MartinsonEveline C VerhulstJürgen GadauJohn H WerrenXu WangXu WangXu WangFrontiers Media S.A.articleMuscidifuraxparasitoid waspbiological controlhouseflylinked-read technologyPacBio sequencingGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Muscidifurax parasitoid wasp biological control housefly linked-read technology PacBio sequencing Genetics QH426-470
spellingShingle	Muscidifurax parasitoid wasp biological control housefly linked-read technology PacBio sequencing Genetics QH426-470 Xiao Xiong Xiao Xiong Yogeshwar D Kelkar Chris J Geden Chao Zhang Yidong Wang Evelien Jongepier Ellen O. Martinson Ellen O. Martinson Eveline C Verhulst Jürgen Gadau John H Werren Xu Wang Xu Wang Xu Wang Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies
description	The parasitoid wasp Muscidifurax raptorellus (Hymenoptera: Pteromalidae) is a gregarious species that has received extensive attention for its potential in biological pest control against house fly, stable fly, and other filth flies. It has a high reproductive capacity and can be reared easily. However, genome assembly is not available for M. raptorellus or any other species in this genus. Previously, we assembled a complete circular mitochondrial genome with a length of 24,717 bp. Here, we assembled and annotated a high-quality nuclear genome of M. raptorellus, using a combination of long-read (104× genome coverage) and short-read (326× genome coverage) sequencing technologies. The assembled genome size is 314 Mbp in 226 contigs, with a 97.9% BUSCO completeness score and a contig N50 of 4.67 Mb, suggesting excellent continuity of this assembly. Our assembly builds the foundation for comparative and evolutionary genomic analysis in the genus of Muscidifurax and possible future biocontrol applications.
format	article
author	Xiao Xiong Xiao Xiong Yogeshwar D Kelkar Chris J Geden Chao Zhang Yidong Wang Evelien Jongepier Ellen O. Martinson Ellen O. Martinson Eveline C Verhulst Jürgen Gadau John H Werren Xu Wang Xu Wang Xu Wang
author_facet	Xiao Xiong Xiao Xiong Yogeshwar D Kelkar Chris J Geden Chao Zhang Yidong Wang Evelien Jongepier Ellen O. Martinson Ellen O. Martinson Eveline C Verhulst Jürgen Gadau John H Werren Xu Wang Xu Wang Xu Wang
author_sort	Xiao Xiong
title	Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies
title_short	Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies
title_full	Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies
title_fullStr	Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies
title_full_unstemmed	Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies
title_sort	long-read assembly and annotation of the parasitoid wasp muscidifurax raptorellus, a biological control agent for filth flies
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/50e1c8c840dc46f485c328405aabb44a
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Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies

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