Exploratory and confirmatory molecular approaches to determine genetically modified status in different crops

Abstract Background One of the parameters required for the assessment of food and feed safety is detection and identification of genetically modified organisms. Legislation in some countries necessitates detection and quantification of modification in food and feed samples. Scientists have raised co...

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Autores principales:	Hanaa Abdel-Sadek Oraby, Nadia Aboul-Ftooh Aboul-Maaty, Hayam Ahmad Al-Sharawi
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Genetically modified organisms GM crops PCR Melting curve RT-PCR Measurement uncertainty Science Q
Acceso en línea:	https://doaj.org/article/50e1ca52f73449dd9bf77d29d09c9acf
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spelling	oai:doaj.org-article:50e1ca52f73449dd9bf77d29d09c9acf2021-11-21T12:39:13ZExploratory and confirmatory molecular approaches to determine genetically modified status in different crops10.1186/s42269-021-00654-32522-8307https://doaj.org/article/50e1ca52f73449dd9bf77d29d09c9acf2021-11-01T00:00:00Zhttps://doi.org/10.1186/s42269-021-00654-3https://doaj.org/toc/2522-8307Abstract Background One of the parameters required for the assessment of food and feed safety is detection and identification of genetically modified organisms. Legislation in some countries necessitates detection and quantification of modification in food and feed samples. Scientists have raised concern about safety of antibiotic resistance marker (ARM) genes used for transformation of crops intended for human and animal consumption. In the present work two molecular approaches have been adopted: one exploratory; for detection and quantification of ARM genes in tested plant samples and the other confirmatory; to determine the specificity/reliability of the obtained results. Results Results revealed that primers for neomycin phosphotransferase (nptII) and aminoglycoside 3″ adenyl-transferase (aadA) were amplified in the majority of the 36 DNA screened samples. Melting curve analysis using hygromycin phosphotransferase (aphIV) gene as target sequence for the fluorescent-based detection approach was performed to ensure reliability and specificity of this procedure and to confirm results obtained by using conventional polymerase chain reaction (PCR). Quantitative RT-PCR results and validation analysis followed, revealed that all of the tested DNA samples were not violating the European legislation for GMOs labeling (0.9%). Conclusions The results fully demonstrated the reproducibility, sensitivity/specificity of the adopted approaches for detection and quantification of even traces of GMO contents. Applying measurement uncertainty (MU) procedures presented in this work will help decision makers to ensure compliance with International Legislation and Regulations. This in its turn will facilitate and enhance trading with countries having compelling labeling regulations.Hanaa Abdel-Sadek OrabyNadia Aboul-Ftooh Aboul-MaatyHayam Ahmad Al-SharawiSpringerOpenarticleGenetically modified organismsGM cropsPCRMelting curveRT-PCRMeasurement uncertaintyScienceQENBulletin of the National Research Centre, Vol 45, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetically modified organisms GM crops PCR Melting curve RT-PCR Measurement uncertainty Science Q
spellingShingle	Genetically modified organisms GM crops PCR Melting curve RT-PCR Measurement uncertainty Science Q Hanaa Abdel-Sadek Oraby Nadia Aboul-Ftooh Aboul-Maaty Hayam Ahmad Al-Sharawi Exploratory and confirmatory molecular approaches to determine genetically modified status in different crops
description	Abstract Background One of the parameters required for the assessment of food and feed safety is detection and identification of genetically modified organisms. Legislation in some countries necessitates detection and quantification of modification in food and feed samples. Scientists have raised concern about safety of antibiotic resistance marker (ARM) genes used for transformation of crops intended for human and animal consumption. In the present work two molecular approaches have been adopted: one exploratory; for detection and quantification of ARM genes in tested plant samples and the other confirmatory; to determine the specificity/reliability of the obtained results. Results Results revealed that primers for neomycin phosphotransferase (nptII) and aminoglycoside 3″ adenyl-transferase (aadA) were amplified in the majority of the 36 DNA screened samples. Melting curve analysis using hygromycin phosphotransferase (aphIV) gene as target sequence for the fluorescent-based detection approach was performed to ensure reliability and specificity of this procedure and to confirm results obtained by using conventional polymerase chain reaction (PCR). Quantitative RT-PCR results and validation analysis followed, revealed that all of the tested DNA samples were not violating the European legislation for GMOs labeling (0.9%). Conclusions The results fully demonstrated the reproducibility, sensitivity/specificity of the adopted approaches for detection and quantification of even traces of GMO contents. Applying measurement uncertainty (MU) procedures presented in this work will help decision makers to ensure compliance with International Legislation and Regulations. This in its turn will facilitate and enhance trading with countries having compelling labeling regulations.
format	article
author	Hanaa Abdel-Sadek Oraby Nadia Aboul-Ftooh Aboul-Maaty Hayam Ahmad Al-Sharawi
author_facet	Hanaa Abdel-Sadek Oraby Nadia Aboul-Ftooh Aboul-Maaty Hayam Ahmad Al-Sharawi
author_sort	Hanaa Abdel-Sadek Oraby
title	Exploratory and confirmatory molecular approaches to determine genetically modified status in different crops
title_short	Exploratory and confirmatory molecular approaches to determine genetically modified status in different crops
title_full	Exploratory and confirmatory molecular approaches to determine genetically modified status in different crops
title_fullStr	Exploratory and confirmatory molecular approaches to determine genetically modified status in different crops
title_full_unstemmed	Exploratory and confirmatory molecular approaches to determine genetically modified status in different crops
title_sort	exploratory and confirmatory molecular approaches to determine genetically modified status in different crops
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/50e1ca52f73449dd9bf77d29d09c9acf
work_keys_str_mv	AT hanaaabdelsadekoraby exploratoryandconfirmatorymolecularapproachestodeterminegeneticallymodifiedstatusindifferentcrops AT nadiaaboulftoohaboulmaaty exploratoryandconfirmatorymolecularapproachestodeterminegeneticallymodifiedstatusindifferentcrops AT hayamahmadalsharawi exploratoryandconfirmatorymolecularapproachestodeterminegeneticallymodifiedstatusindifferentcrops
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Exploratory and confirmatory molecular approaches to determine genetically modified status in different crops

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