Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain

Abstract Background The naked mole-rat (NMR) is the longest-lived rodent with a maximum lifespan of more than 37 years and shows a negligible senescence phenotype, suggesting that tissue stem cells of NMRs are highly capable of maintaining homeostasis. However, the properties of NMR tissue stem cell...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuki Yamamura, Yoshimi Kawamura, Yuki Oiwa, Kaori Oka, Nobuyuki Onishi, Hideyuki Saya, Kyoko Miura
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Naked mole-rat Neural stem cell Cell cycle Cell proliferation DNA damage response Pathology RB1-214
Acceso en línea:	https://doaj.org/article/50fb4623413c418db821bffc07ceb691
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:50fb4623413c418db821bffc07ceb691
record_format	dspace
spelling	oai:doaj.org-article:50fb4623413c418db821bffc07ceb6912021-11-08T10:59:05ZIsolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain10.1186/s41232-021-00182-71880-8190https://doaj.org/article/50fb4623413c418db821bffc07ceb6912021-11-01T00:00:00Zhttps://doi.org/10.1186/s41232-021-00182-7https://doaj.org/toc/1880-8190Abstract Background The naked mole-rat (NMR) is the longest-lived rodent with a maximum lifespan of more than 37 years and shows a negligible senescence phenotype, suggesting that tissue stem cells of NMRs are highly capable of maintaining homeostasis. However, the properties of NMR tissue stem cells, including neural stem cells (NSCs), are largely unclear. Methods Neural stem/progenitor cells (NS/PCs) were isolated from the subventricular zone of the neonate NMR brain (NMR-NS/PCs) and cultured in neurosphere and adherent culture conditions. Expression of NSC markers and markers of neurons, astrocytes, and oligodendrocytes was analyzed by immunocytochemistry. In adherent culture conditions, the proliferation rate and cell cycle of NMR-NS/PCs were assessed and compared with those of NS/PCs from mice (mouse-NS/PCs). The DNA damage response to γ-irradiation was analyzed by immunocytochemistry and reverse transcription-quantitative PCR. Results NMR-NS/PCs expressed several NSC markers and differentiated into neurons, astrocytes, and oligodendrocytes. NMR-NS/PCs proliferated markedly slower than mouse-NS/PCs, and a higher percentage of NMR-NS/PCs than mouse-NS/PCs was in G0/G1 phase. Notably, upon γ-irradiation, NMR-NS/PCs exhibited a faster initiation of the DNA damage response and were less prone to dying than mouse-NS/PCs. Conclusions NMR-NS/PCs were successfully isolated and cultured. The slow proliferation of NMR-NS/PCs and their resistance to DNA damage may help to prevent stem cell exhaustion in the brain during the long lifespan of NMRs. Our findings provide novel insights into the mechanism underlying delayed aging of NMRs. Further analysis of NMR tissue stem cells may lead to the development of new strategies that can prevent aging in humans.Yuki YamamuraYoshimi KawamuraYuki OiwaKaori OkaNobuyuki OnishiHideyuki SayaKyoko MiuraBMCarticleNaked mole-ratNeural stem cellCell cycleCell proliferationDNA damage responsePathologyRB1-214ENInflammation and Regeneration, Vol 41, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Naked mole-rat Neural stem cell Cell cycle Cell proliferation DNA damage response Pathology RB1-214
spellingShingle	Naked mole-rat Neural stem cell Cell cycle Cell proliferation DNA damage response Pathology RB1-214 Yuki Yamamura Yoshimi Kawamura Yuki Oiwa Kaori Oka Nobuyuki Onishi Hideyuki Saya Kyoko Miura Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain
description	Abstract Background The naked mole-rat (NMR) is the longest-lived rodent with a maximum lifespan of more than 37 years and shows a negligible senescence phenotype, suggesting that tissue stem cells of NMRs are highly capable of maintaining homeostasis. However, the properties of NMR tissue stem cells, including neural stem cells (NSCs), are largely unclear. Methods Neural stem/progenitor cells (NS/PCs) were isolated from the subventricular zone of the neonate NMR brain (NMR-NS/PCs) and cultured in neurosphere and adherent culture conditions. Expression of NSC markers and markers of neurons, astrocytes, and oligodendrocytes was analyzed by immunocytochemistry. In adherent culture conditions, the proliferation rate and cell cycle of NMR-NS/PCs were assessed and compared with those of NS/PCs from mice (mouse-NS/PCs). The DNA damage response to γ-irradiation was analyzed by immunocytochemistry and reverse transcription-quantitative PCR. Results NMR-NS/PCs expressed several NSC markers and differentiated into neurons, astrocytes, and oligodendrocytes. NMR-NS/PCs proliferated markedly slower than mouse-NS/PCs, and a higher percentage of NMR-NS/PCs than mouse-NS/PCs was in G0/G1 phase. Notably, upon γ-irradiation, NMR-NS/PCs exhibited a faster initiation of the DNA damage response and were less prone to dying than mouse-NS/PCs. Conclusions NMR-NS/PCs were successfully isolated and cultured. The slow proliferation of NMR-NS/PCs and their resistance to DNA damage may help to prevent stem cell exhaustion in the brain during the long lifespan of NMRs. Our findings provide novel insights into the mechanism underlying delayed aging of NMRs. Further analysis of NMR tissue stem cells may lead to the development of new strategies that can prevent aging in humans.
format	article
author	Yuki Yamamura Yoshimi Kawamura Yuki Oiwa Kaori Oka Nobuyuki Onishi Hideyuki Saya Kyoko Miura
author_facet	Yuki Yamamura Yoshimi Kawamura Yuki Oiwa Kaori Oka Nobuyuki Onishi Hideyuki Saya Kyoko Miura
author_sort	Yuki Yamamura
title	Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain
title_short	Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain
title_full	Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain
title_fullStr	Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain
title_full_unstemmed	Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain
title_sort	isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain
publisher	BMC
publishDate	2021
url	https://doaj.org/article/50fb4623413c418db821bffc07ceb691
work_keys_str_mv	AT yukiyamamura isolationandcharacterizationofneuralstemprogenitorcellsinthesubventricularzoneofthenakedmoleratbrain AT yoshimikawamura isolationandcharacterizationofneuralstemprogenitorcellsinthesubventricularzoneofthenakedmoleratbrain AT yukioiwa isolationandcharacterizationofneuralstemprogenitorcellsinthesubventricularzoneofthenakedmoleratbrain AT kaorioka isolationandcharacterizationofneuralstemprogenitorcellsinthesubventricularzoneofthenakedmoleratbrain AT nobuyukionishi isolationandcharacterizationofneuralstemprogenitorcellsinthesubventricularzoneofthenakedmoleratbrain AT hideyukisaya isolationandcharacterizationofneuralstemprogenitorcellsinthesubventricularzoneofthenakedmoleratbrain AT kyokomiura isolationandcharacterizationofneuralstemprogenitorcellsinthesubventricularzoneofthenakedmoleratbrain
_version_	1718442422457860096

Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain

Ejemplares similares