Seroepidemiology of human polyomaviruses.

In addition to the previously characterized viruses BK and JC, three new human polyomaviruses (Pys) have been recently identified: KIV, WUV, and Merkel Cell Py (MCV). Using an ELISA employing recombinant VP1 capsid proteins, we have determined the seroprevalence of KIV, WUV, and MCV, along with BKV...

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Autores principales:	Jaime M Kean, Suchitra Rao, Michael Wang, Robert L Garcea
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5100236fe1a948f98ce548bde6e515df
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spelling	oai:doaj.org-article:5100236fe1a948f98ce548bde6e515df2021-11-25T05:47:10ZSeroepidemiology of human polyomaviruses.1553-73661553-737410.1371/journal.ppat.1000363https://doaj.org/article/5100236fe1a948f98ce548bde6e515df2009-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19325891/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374In addition to the previously characterized viruses BK and JC, three new human polyomaviruses (Pys) have been recently identified: KIV, WUV, and Merkel Cell Py (MCV). Using an ELISA employing recombinant VP1 capsid proteins, we have determined the seroprevalence of KIV, WUV, and MCV, along with BKV and JCV, and the monkey viruses SV40 and LPV. Soluble VP1 proteins were used to assess crossreactivity between viruses. We found the seroprevalence (+/- 1%) in healthy adult blood donors (1501) was SV40 (9%), BKV (82%), JCV (39%), LPV (15%), KIV (55%), WUV (69%), MCV strain 350 (25%), and MCV strain 339 (42%). Competition assays detected no sero-crossreactivity between the VP1 proteins of LPV or MCV or between WUV and KIV. There was considerable sero-crossreactivity between SV40 and BKV, and to a lesser extent, between SV40 and JCV VP1 proteins. After correcting for crossreactivity, the SV40 seroprevalence was approximately 2%. The seroprevalence in children under 21 years of age (n = 721) for all Pys was similar to that of the adult population, suggesting that primary exposure to these viruses likely occurs in childhood.Jaime M KeanSuchitra RaoMichael WangRobert L GarceaPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 3, p e1000363 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Jaime M Kean Suchitra Rao Michael Wang Robert L Garcea Seroepidemiology of human polyomaviruses.
description	In addition to the previously characterized viruses BK and JC, three new human polyomaviruses (Pys) have been recently identified: KIV, WUV, and Merkel Cell Py (MCV). Using an ELISA employing recombinant VP1 capsid proteins, we have determined the seroprevalence of KIV, WUV, and MCV, along with BKV and JCV, and the monkey viruses SV40 and LPV. Soluble VP1 proteins were used to assess crossreactivity between viruses. We found the seroprevalence (+/- 1%) in healthy adult blood donors (1501) was SV40 (9%), BKV (82%), JCV (39%), LPV (15%), KIV (55%), WUV (69%), MCV strain 350 (25%), and MCV strain 339 (42%). Competition assays detected no sero-crossreactivity between the VP1 proteins of LPV or MCV or between WUV and KIV. There was considerable sero-crossreactivity between SV40 and BKV, and to a lesser extent, between SV40 and JCV VP1 proteins. After correcting for crossreactivity, the SV40 seroprevalence was approximately 2%. The seroprevalence in children under 21 years of age (n = 721) for all Pys was similar to that of the adult population, suggesting that primary exposure to these viruses likely occurs in childhood.
format	article
author	Jaime M Kean Suchitra Rao Michael Wang Robert L Garcea
author_facet	Jaime M Kean Suchitra Rao Michael Wang Robert L Garcea
author_sort	Jaime M Kean
title	Seroepidemiology of human polyomaviruses.
title_short	Seroepidemiology of human polyomaviruses.
title_full	Seroepidemiology of human polyomaviruses.
title_fullStr	Seroepidemiology of human polyomaviruses.
title_full_unstemmed	Seroepidemiology of human polyomaviruses.
title_sort	seroepidemiology of human polyomaviruses.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/5100236fe1a948f98ce548bde6e515df
work_keys_str_mv	AT jaimemkean seroepidemiologyofhumanpolyomaviruses AT suchitrarao seroepidemiologyofhumanpolyomaviruses AT michaelwang seroepidemiologyofhumanpolyomaviruses AT robertlgarcea seroepidemiologyofhumanpolyomaviruses
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Seroepidemiology of human polyomaviruses.

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