Cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges

Abstract Ripe Cara Cara sweet orange contains 25 times as much carotenoids in flesh as Newhall sweet orange, due to high accumulation of carotenes, mainly phytoene, lycopene and phytofluene. Only yellow globular chromoplasts were observed in Newhall flesh. Distinct yellow globular and red elongated...

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Autores principales:	Peng-jun Lu, Chun-yan Wang, Ting-ting Yin, Si-lin Zhong, Don Grierson, Kun-song Chen, Chang-jie Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5108c763a42e4b3682f6036df840a6c5
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spelling	oai:doaj.org-article:5108c763a42e4b3682f6036df840a6c52021-12-02T12:31:46ZCytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges10.1038/s41598-017-00898-y2045-2322https://doaj.org/article/5108c763a42e4b3682f6036df840a6c52017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00898-yhttps://doaj.org/toc/2045-2322Abstract Ripe Cara Cara sweet orange contains 25 times as much carotenoids in flesh as Newhall sweet orange, due to high accumulation of carotenes, mainly phytoene, lycopene and phytofluene. Only yellow globular chromoplasts were observed in Newhall flesh. Distinct yellow globular and red elongated crystalline chromoplasts were found in Cara Cara but only one type of chromoplast was present in each cell. The red crystalline chromoplasts contained lycopene as a dominant carotenoid and were associated with characteristic carotenoid sequestering structures. The increased accumulation of linear carotenes in Cara Cara is not explained by differences in expression of all 18 carotenogenic genes or gene family members examined, or sequence or abundance of mRNAs from phytoene synthase (PSY) and chromoplast-specific lycopene β-cyclase (CYCB) alleles. 2-(4-Chlorophenylthio)-triethylamine hydrochloride (CPTA) enhanced lycopene accumulation and induced occurrence of red crystalline chromoplasts in cultured Newhall juice vesicles, indicating that carotenoid synthesis and accumulation can directly affect chromoplast differentiation and structure. Norflurazon (NFZ) treatment resulted in high accumulation of phytoene and phytofluene in both oranges, and the biosynthetic activity upstream of phytoene desaturase was similar in Newhall and Cara Cara. Possible mechanisms for high carotene accumulation and unique development of red crystalline chromoplasts in Cara Cara are discussed.Peng-jun LuChun-yan WangTing-ting YinSi-lin ZhongDon GriersonKun-song ChenChang-jie XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng-jun Lu Chun-yan Wang Ting-ting Yin Si-lin Zhong Don Grierson Kun-song Chen Chang-jie Xu Cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges
description	Abstract Ripe Cara Cara sweet orange contains 25 times as much carotenoids in flesh as Newhall sweet orange, due to high accumulation of carotenes, mainly phytoene, lycopene and phytofluene. Only yellow globular chromoplasts were observed in Newhall flesh. Distinct yellow globular and red elongated crystalline chromoplasts were found in Cara Cara but only one type of chromoplast was present in each cell. The red crystalline chromoplasts contained lycopene as a dominant carotenoid and were associated with characteristic carotenoid sequestering structures. The increased accumulation of linear carotenes in Cara Cara is not explained by differences in expression of all 18 carotenogenic genes or gene family members examined, or sequence or abundance of mRNAs from phytoene synthase (PSY) and chromoplast-specific lycopene β-cyclase (CYCB) alleles. 2-(4-Chlorophenylthio)-triethylamine hydrochloride (CPTA) enhanced lycopene accumulation and induced occurrence of red crystalline chromoplasts in cultured Newhall juice vesicles, indicating that carotenoid synthesis and accumulation can directly affect chromoplast differentiation and structure. Norflurazon (NFZ) treatment resulted in high accumulation of phytoene and phytofluene in both oranges, and the biosynthetic activity upstream of phytoene desaturase was similar in Newhall and Cara Cara. Possible mechanisms for high carotene accumulation and unique development of red crystalline chromoplasts in Cara Cara are discussed.
format	article
author	Peng-jun Lu Chun-yan Wang Ting-ting Yin Si-lin Zhong Don Grierson Kun-song Chen Chang-jie Xu
author_facet	Peng-jun Lu Chun-yan Wang Ting-ting Yin Si-lin Zhong Don Grierson Kun-song Chen Chang-jie Xu
author_sort	Peng-jun Lu
title	Cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges
title_short	Cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges
title_full	Cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges
title_fullStr	Cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges
title_full_unstemmed	Cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges
title_sort	cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5108c763a42e4b3682f6036df840a6c5
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Cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges

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