An atypical strictosidine synthase, OsSTRL2, plays key roles in anther development and pollen wall formation in rice

Abstract Strictosidine synthase (STR) plays an important role in the biosynthesis of terpenoid indole alkaloids (TIAs) and is expressed in a range of active meristematic tissues of higher plants. STR proteins are involved in different physiological and biochemical pathways. However, the function of...

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Autores principales:	Ting Zou, Shuangcheng Li, Mingxing Liu, Tao Wang, Qiao Xiao, Dan Chen, Qiao Li, Yanling Liang, Jun Zhu, Yueyang Liang, Qiming Deng, Shiquan Wang, Aiping Zheng, Lingxia Wang, Ping Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/510b5e707bba4568b4adae3e80c5b0d3
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spelling	oai:doaj.org-article:510b5e707bba4568b4adae3e80c5b0d32021-12-02T16:06:56ZAn atypical strictosidine synthase, OsSTRL2, plays key roles in anther development and pollen wall formation in rice10.1038/s41598-017-07064-42045-2322https://doaj.org/article/510b5e707bba4568b4adae3e80c5b0d32017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07064-4https://doaj.org/toc/2045-2322Abstract Strictosidine synthase (STR) plays an important role in the biosynthesis of terpenoid indole alkaloids (TIAs) and is expressed in a range of active meristematic tissues of higher plants. STR proteins are involved in different physiological and biochemical pathways. However, the function of STR proteins in rice development remains poorly understood. In this study, we identified 21 possible STR-like (OsSTRL) family members in rice genome and found that only one gene, OsSTRL2, exhibited a pre-emergency specific florescence expression pattern. Tissue-specific expression profile analysis, β-glucuronidase histochemical (GUS) staining and RNA in situ hybridization confirmed that OsSTRL2 was highly expressed in tapetal cells and microspores. Comparative protein sequence analysis indicated that OsSTRL2 lacked the key catalytic residue found in a typical STR (STR1), although it possessed conserved β-propellers and α-helices formed the basic structure of STR1. OsSTRL2 knockout mutant resulted to male sterility because of the defects in anther development and pollen wall formation. Subcellular localization of OsSTRL2-YFP revealed that the OsSTRL2 protein was primarily localized in the endoplasmic reticulum (ER). Therefore, OsSTRL2 is an atypical strictosidine synthase that plays crucial roles in regulating anther development and pollen wall formation in rice.Ting ZouShuangcheng LiMingxing LiuTao WangQiao XiaoDan ChenQiao LiYanling LiangJun ZhuYueyang LiangQiming DengShiquan WangAiping ZhengLingxia WangPing LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ting Zou Shuangcheng Li Mingxing Liu Tao Wang Qiao Xiao Dan Chen Qiao Li Yanling Liang Jun Zhu Yueyang Liang Qiming Deng Shiquan Wang Aiping Zheng Lingxia Wang Ping Li An atypical strictosidine synthase, OsSTRL2, plays key roles in anther development and pollen wall formation in rice
description	Abstract Strictosidine synthase (STR) plays an important role in the biosynthesis of terpenoid indole alkaloids (TIAs) and is expressed in a range of active meristematic tissues of higher plants. STR proteins are involved in different physiological and biochemical pathways. However, the function of STR proteins in rice development remains poorly understood. In this study, we identified 21 possible STR-like (OsSTRL) family members in rice genome and found that only one gene, OsSTRL2, exhibited a pre-emergency specific florescence expression pattern. Tissue-specific expression profile analysis, β-glucuronidase histochemical (GUS) staining and RNA in situ hybridization confirmed that OsSTRL2 was highly expressed in tapetal cells and microspores. Comparative protein sequence analysis indicated that OsSTRL2 lacked the key catalytic residue found in a typical STR (STR1), although it possessed conserved β-propellers and α-helices formed the basic structure of STR1. OsSTRL2 knockout mutant resulted to male sterility because of the defects in anther development and pollen wall formation. Subcellular localization of OsSTRL2-YFP revealed that the OsSTRL2 protein was primarily localized in the endoplasmic reticulum (ER). Therefore, OsSTRL2 is an atypical strictosidine synthase that plays crucial roles in regulating anther development and pollen wall formation in rice.
format	article
author	Ting Zou Shuangcheng Li Mingxing Liu Tao Wang Qiao Xiao Dan Chen Qiao Li Yanling Liang Jun Zhu Yueyang Liang Qiming Deng Shiquan Wang Aiping Zheng Lingxia Wang Ping Li
author_facet	Ting Zou Shuangcheng Li Mingxing Liu Tao Wang Qiao Xiao Dan Chen Qiao Li Yanling Liang Jun Zhu Yueyang Liang Qiming Deng Shiquan Wang Aiping Zheng Lingxia Wang Ping Li
author_sort	Ting Zou
title	An atypical strictosidine synthase, OsSTRL2, plays key roles in anther development and pollen wall formation in rice
title_short	An atypical strictosidine synthase, OsSTRL2, plays key roles in anther development and pollen wall formation in rice
title_full	An atypical strictosidine synthase, OsSTRL2, plays key roles in anther development and pollen wall formation in rice
title_fullStr	An atypical strictosidine synthase, OsSTRL2, plays key roles in anther development and pollen wall formation in rice
title_full_unstemmed	An atypical strictosidine synthase, OsSTRL2, plays key roles in anther development and pollen wall formation in rice
title_sort	atypical strictosidine synthase, osstrl2, plays key roles in anther development and pollen wall formation in rice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/510b5e707bba4568b4adae3e80c5b0d3
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An atypical strictosidine synthase, OsSTRL2, plays key roles in anther development and pollen wall formation in rice

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