Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice

Abstract. Nurhasanah, Ramita, Supriyanto B, Sunaryo W. 2019. Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice. Biodiversitas 20: 2288-2294. Iron toxicity is major abiotic stress that limits rice production in most tropical regions. A high...

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Autores principales:	NURHASANAH NURHASANAH, RAMITA, BAMBANG SUPRIYANTO, WIDI SUNARYO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	east kalimantan, embryo somatic, in-vitro selection, iron stress, local rice Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/510d56cfb282470ba917cae2c36cd607
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spelling	oai:doaj.org-article:510d56cfb282470ba917cae2c36cd6072021-11-21T21:43:55ZSomatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice1412-033X2085-472210.13057/biodiv/d200825https://doaj.org/article/510d56cfb282470ba917cae2c36cd6072019-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4013https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Nurhasanah, Ramita, Supriyanto B, Sunaryo W. 2019. Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice. Biodiversitas 20: 2288-2294. Iron toxicity is major abiotic stress that limits rice production in most tropical regions. A high iron concentration will lead to the abnormal growth and development, decreased yield, and even death of the plant. Compared to other recommended treatments to overcome iron toxicity problems, the use of tolerant cultivars is considered the most effective and efficient approach. In this study, the tolerant character against iron stress was developed using East Kalimantan local rice cultivars through somaclonal variation and in vitro selection. The somatic embryos from four indica rice cultivars, Mayas Pancing, Gedagai, Siam, and Serai, were selected in iron-containing media. Five levels of iron concentrations were applied in the selection media 0; 27.8; 55.6; 83.4, and 111.2 mg.L-1 of FeSO4.7H2O. The media acidity was adjusted to pH 4.00 for iron-containing media and 5.8 for the zero iron concentration (without iron). The results showed that the survival ability of embryonic calli (EC) was significantly influenced by the level of iron concentration. The absence of an iron source in the selection media led to lower EC survival. The percentage of EC survival ability was even lower than that of the presence of two folds of normal iron concentration (55.6 mgL-1 of FeSO4.7H2O). Three and four folds of the normal iron concentration (83.6 mg.L-1 and 111.2 mg.L-1 FeSO4.7H2O) mostly produced the lowest percentage of EC survival for the four local rice cultivars. Mayas Pancing had better tolerance to iron stress and the highest EC survival number compared to other genotypes. The role of genotype was clearly observed in the regeneration ability of EC. Plantlets were only produced on the EC from the Serai Gunung and Siam cultivars.NURHASANAH NURHASANAHRAMITABAMBANG SUPRIYANTOWIDI SUNARYOMBI & UNS Soloarticleeast kalimantan, embryo somatic, in-vitro selection, iron stress, local riceBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	east kalimantan, embryo somatic, in-vitro selection, iron stress, local rice Biology (General) QH301-705.5
spellingShingle	east kalimantan, embryo somatic, in-vitro selection, iron stress, local rice Biology (General) QH301-705.5 NURHASANAH NURHASANAH RAMITA BAMBANG SUPRIYANTO WIDI SUNARYO Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice
description	Abstract. Nurhasanah, Ramita, Supriyanto B, Sunaryo W. 2019. Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice. Biodiversitas 20: 2288-2294. Iron toxicity is major abiotic stress that limits rice production in most tropical regions. A high iron concentration will lead to the abnormal growth and development, decreased yield, and even death of the plant. Compared to other recommended treatments to overcome iron toxicity problems, the use of tolerant cultivars is considered the most effective and efficient approach. In this study, the tolerant character against iron stress was developed using East Kalimantan local rice cultivars through somaclonal variation and in vitro selection. The somatic embryos from four indica rice cultivars, Mayas Pancing, Gedagai, Siam, and Serai, were selected in iron-containing media. Five levels of iron concentrations were applied in the selection media 0; 27.8; 55.6; 83.4, and 111.2 mg.L-1 of FeSO4.7H2O. The media acidity was adjusted to pH 4.00 for iron-containing media and 5.8 for the zero iron concentration (without iron). The results showed that the survival ability of embryonic calli (EC) was significantly influenced by the level of iron concentration. The absence of an iron source in the selection media led to lower EC survival. The percentage of EC survival ability was even lower than that of the presence of two folds of normal iron concentration (55.6 mgL-1 of FeSO4.7H2O). Three and four folds of the normal iron concentration (83.6 mg.L-1 and 111.2 mg.L-1 FeSO4.7H2O) mostly produced the lowest percentage of EC survival for the four local rice cultivars. Mayas Pancing had better tolerance to iron stress and the highest EC survival number compared to other genotypes. The role of genotype was clearly observed in the regeneration ability of EC. Plantlets were only produced on the EC from the Serai Gunung and Siam cultivars.
format	article
author	NURHASANAH NURHASANAH RAMITA BAMBANG SUPRIYANTO WIDI SUNARYO
author_facet	NURHASANAH NURHASANAH RAMITA BAMBANG SUPRIYANTO WIDI SUNARYO
author_sort	NURHASANAH NURHASANAH
title	Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice
title_short	Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice
title_full	Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice
title_fullStr	Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice
title_full_unstemmed	Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice
title_sort	somatic embryos response against iron stress in in-vitro culture condition of east kalimantan (indonesia) rice
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/510d56cfb282470ba917cae2c36cd607
work_keys_str_mv	AT nurhasanahnurhasanah somaticembryosresponseagainstironstressininvitrocultureconditionofeastkalimantanindonesiarice AT ramita somaticembryosresponseagainstironstressininvitrocultureconditionofeastkalimantanindonesiarice AT bambangsupriyanto somaticembryosresponseagainstironstressininvitrocultureconditionofeastkalimantanindonesiarice AT widisunaryo somaticembryosresponseagainstironstressininvitrocultureconditionofeastkalimantanindonesiarice
_version_	1718418640868474880

Somatic embryos response against iron stress in in-vitro culture condition of East Kalimantan (Indonesia) rice

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