Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment

CENP-A histone variants replace histones H3 at centromeres. Here the authors use a single-chain antibody fragment (scFv) to stabilize human CENP-A nucleosome containing a native α-satellite DNA and solved its structure by cryo-EM to 2.6 Å resolution, providing insight into the structure and function...

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Autores principales:	Bing-Rui Zhou, K. N. Sathish Yadav, Mario Borgnia, Jingjun Hong, Baohua Cao, Ada L. Olins, Donald E. Olins, Yawen Bai, Ping Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/510fb8f60bd045f78e1fc1422101e0d3
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spelling	oai:doaj.org-article:510fb8f60bd045f78e1fc1422101e0d32021-12-02T17:01:26ZAtomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment10.1038/s41467-019-10247-42041-1723https://doaj.org/article/510fb8f60bd045f78e1fc1422101e0d32019-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10247-4https://doaj.org/toc/2041-1723CENP-A histone variants replace histones H3 at centromeres. Here the authors use a single-chain antibody fragment (scFv) to stabilize human CENP-A nucleosome containing a native α-satellite DNA and solved its structure by cryo-EM to 2.6 Å resolution, providing insight into the structure and function of the CENP-A nucleosome.Bing-Rui ZhouK. N. Sathish YadavMario BorgniaJingjun HongBaohua CaoAda L. OlinsDonald E. OlinsYawen BaiPing ZhangNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-7 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Bing-Rui Zhou K. N. Sathish Yadav Mario Borgnia Jingjun Hong Baohua Cao Ada L. Olins Donald E. Olins Yawen Bai Ping Zhang Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment
description	CENP-A histone variants replace histones H3 at centromeres. Here the authors use a single-chain antibody fragment (scFv) to stabilize human CENP-A nucleosome containing a native α-satellite DNA and solved its structure by cryo-EM to 2.6 Å resolution, providing insight into the structure and function of the CENP-A nucleosome.
format	article
author	Bing-Rui Zhou K. N. Sathish Yadav Mario Borgnia Jingjun Hong Baohua Cao Ada L. Olins Donald E. Olins Yawen Bai Ping Zhang
author_facet	Bing-Rui Zhou K. N. Sathish Yadav Mario Borgnia Jingjun Hong Baohua Cao Ada L. Olins Donald E. Olins Yawen Bai Ping Zhang
author_sort	Bing-Rui Zhou
title	Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment
title_short	Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment
title_full	Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment
title_fullStr	Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment
title_full_unstemmed	Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment
title_sort	atomic resolution cryo-em structure of a native-like cenp-a nucleosome aided by an antibody fragment
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/510fb8f60bd045f78e1fc1422101e0d3
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Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment

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