Alcohol Acyltransferase Is Involved in the Biosynthesis of C6 Esters in Apricot (Prunus armeniaca L.) Fruit

Short-chain esters derived from fatty acid contribute to the characteristic flavor of apricot fruit, and the biosynthesis of these compounds in fruit is catalyzed by alcohol acyltransferase (AAT). In this work, we investigated the AAT gene family via genome-wide scanning, and three AAT loci were ide...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wanhai Zhou, Wenbin Kong, Can Yang, Ruizhang Feng, Wanpeng Xi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Prunus armeniaca L. alcohol acyltransferase fruit aroma ester apricot Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/511857bb829c4dab9c075b054002ade5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:511857bb829c4dab9c075b054002ade5
record_format	dspace
spelling	oai:doaj.org-article:511857bb829c4dab9c075b054002ade52021-11-17T15:58:26ZAlcohol Acyltransferase Is Involved in the Biosynthesis of C6 Esters in Apricot (Prunus armeniaca L.) Fruit1664-462X10.3389/fpls.2021.763139https://doaj.org/article/511857bb829c4dab9c075b054002ade52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.763139/fullhttps://doaj.org/toc/1664-462XShort-chain esters derived from fatty acid contribute to the characteristic flavor of apricot fruit, and the biosynthesis of these compounds in fruit is catalyzed by alcohol acyltransferase (AAT). In this work, we investigated the AAT gene family via genome-wide scanning, and three AAT loci were identified in different linkage groups (LGs), with PaAAT1 (PARG22907m01) in LG7, PaAAT2 (PARG15279m01) in LG4, and PaAAT3 (PARG22697m01) in LG6. Phylogenetic analysis showed that PaAAT1 belongs to clade 3, while PaAAT2 and PaAAT3 belong to clade 1 and clade 2, respectively. In contrast, the three AAT genes present different expression patterns. Only PaAAT1 exhibited distinct patterns of fruit-specific expression, and the expression of PaAAT1 sharply increased during fruit ripening, which is consistent with the abundance of C4–C6 esters such as (E)-2-hexenyl acetate and (Z)-3-hexenyl acetate. The transient overexpression of PaAAT1 in Katy (KT) apricot fruit resulted in a remarkable decrease in hexenol, (E)-2-hexenol, and (Z)-3-hexenol levels while significantly increasing the corresponding acetate production (p < 0.01). A substrate assay revealed that the PaAAT1 protein enzyme can produce hexenyl acetate, (E)-2-hexenyl acetate, and (Z)-3-hexenyl acetate when C6 alcohols are used as substrates for the reaction. Taken together, these results indicate that PaAAT1 plays a crucial role in the production of C6 esters in apricot fruit during ripening.Wanhai ZhouWenbin KongCan YangRuizhang FengWanpeng XiFrontiers Media S.A.articlePrunus armeniaca L.alcohol acyltransferasefruit aromaesterapricotPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Prunus armeniaca L. alcohol acyltransferase fruit aroma ester apricot Plant culture SB1-1110
spellingShingle	Prunus armeniaca L. alcohol acyltransferase fruit aroma ester apricot Plant culture SB1-1110 Wanhai Zhou Wenbin Kong Can Yang Ruizhang Feng Wanpeng Xi Alcohol Acyltransferase Is Involved in the Biosynthesis of C6 Esters in Apricot (Prunus armeniaca L.) Fruit
description	Short-chain esters derived from fatty acid contribute to the characteristic flavor of apricot fruit, and the biosynthesis of these compounds in fruit is catalyzed by alcohol acyltransferase (AAT). In this work, we investigated the AAT gene family via genome-wide scanning, and three AAT loci were identified in different linkage groups (LGs), with PaAAT1 (PARG22907m01) in LG7, PaAAT2 (PARG15279m01) in LG4, and PaAAT3 (PARG22697m01) in LG6. Phylogenetic analysis showed that PaAAT1 belongs to clade 3, while PaAAT2 and PaAAT3 belong to clade 1 and clade 2, respectively. In contrast, the three AAT genes present different expression patterns. Only PaAAT1 exhibited distinct patterns of fruit-specific expression, and the expression of PaAAT1 sharply increased during fruit ripening, which is consistent with the abundance of C4–C6 esters such as (E)-2-hexenyl acetate and (Z)-3-hexenyl acetate. The transient overexpression of PaAAT1 in Katy (KT) apricot fruit resulted in a remarkable decrease in hexenol, (E)-2-hexenol, and (Z)-3-hexenol levels while significantly increasing the corresponding acetate production (p < 0.01). A substrate assay revealed that the PaAAT1 protein enzyme can produce hexenyl acetate, (E)-2-hexenyl acetate, and (Z)-3-hexenyl acetate when C6 alcohols are used as substrates for the reaction. Taken together, these results indicate that PaAAT1 plays a crucial role in the production of C6 esters in apricot fruit during ripening.
format	article
author	Wanhai Zhou Wenbin Kong Can Yang Ruizhang Feng Wanpeng Xi
author_facet	Wanhai Zhou Wenbin Kong Can Yang Ruizhang Feng Wanpeng Xi
author_sort	Wanhai Zhou
title	Alcohol Acyltransferase Is Involved in the Biosynthesis of C6 Esters in Apricot (Prunus armeniaca L.) Fruit
title_short	Alcohol Acyltransferase Is Involved in the Biosynthesis of C6 Esters in Apricot (Prunus armeniaca L.) Fruit
title_full	Alcohol Acyltransferase Is Involved in the Biosynthesis of C6 Esters in Apricot (Prunus armeniaca L.) Fruit
title_fullStr	Alcohol Acyltransferase Is Involved in the Biosynthesis of C6 Esters in Apricot (Prunus armeniaca L.) Fruit
title_full_unstemmed	Alcohol Acyltransferase Is Involved in the Biosynthesis of C6 Esters in Apricot (Prunus armeniaca L.) Fruit
title_sort	alcohol acyltransferase is involved in the biosynthesis of c6 esters in apricot (prunus armeniaca l.) fruit
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/511857bb829c4dab9c075b054002ade5
work_keys_str_mv	AT wanhaizhou alcoholacyltransferaseisinvolvedinthebiosynthesisofc6estersinapricotprunusarmeniacalfruit AT wenbinkong alcoholacyltransferaseisinvolvedinthebiosynthesisofc6estersinapricotprunusarmeniacalfruit AT canyang alcoholacyltransferaseisinvolvedinthebiosynthesisofc6estersinapricotprunusarmeniacalfruit AT ruizhangfeng alcoholacyltransferaseisinvolvedinthebiosynthesisofc6estersinapricotprunusarmeniacalfruit AT wanpengxi alcoholacyltransferaseisinvolvedinthebiosynthesisofc6estersinapricotprunusarmeniacalfruit
_version_	1718425414387367936

Alcohol Acyltransferase Is Involved in the Biosynthesis of C6 Esters in Apricot (Prunus armeniaca L.) Fruit

Ejemplares similares