Adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies.

The global population remains vulnerable in the face of the next pandemic influenza virus outbreak, and reformulated vaccinations are administered annually to manage seasonal epidemics. Therefore, development of a new generation of vaccines is needed to generate broad and persistent immunity to infl...

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Autores principales:	Peter H Goff, Dirk Eggink, Christopher W Seibert, Rong Hai, Luis Martínez-Gil, Florian Krammer, Peter Palese
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/512980efeaaa4d36b2f9273302d7b0ea
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spelling	oai:doaj.org-article:512980efeaaa4d36b2f9273302d7b0ea2021-11-18T08:48:05ZAdjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies.1932-620310.1371/journal.pone.0079194https://doaj.org/article/512980efeaaa4d36b2f9273302d7b0ea2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24223176/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The global population remains vulnerable in the face of the next pandemic influenza virus outbreak, and reformulated vaccinations are administered annually to manage seasonal epidemics. Therefore, development of a new generation of vaccines is needed to generate broad and persistent immunity to influenza viruses. Here, we describe three adjuvants that enhance the induction of stalk-directed antibodies against heterologous and heterosubtypic influenza viruses when administered with chimeric HA proteins. Addavax, an MF59-like nanoemulsion, poly(I:C), and an RNA hairpin derived from Sendai virus (SeV) Cantell were efficacious intramuscularly. The SeV RNA and poly(I:C) also proved to be effective respiratory mucosal adjuvants. Although the quantity and quality of antibodies induced by the adjuvants varied, immunized mice demonstrated comparable levels of protection against challenge with influenza A viruses on the basis of HA stalk reactivity. Finally, we present that intranasally, but not intramuscularly, administered chimeric HA proteins induce mucosal IgA antibodies directed at the HA stalk.Peter H GoffDirk EgginkChristopher W SeibertRong HaiLuis Martínez-GilFlorian KrammerPeter PalesePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79194 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peter H Goff Dirk Eggink Christopher W Seibert Rong Hai Luis Martínez-Gil Florian Krammer Peter Palese Adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies.
description	The global population remains vulnerable in the face of the next pandemic influenza virus outbreak, and reformulated vaccinations are administered annually to manage seasonal epidemics. Therefore, development of a new generation of vaccines is needed to generate broad and persistent immunity to influenza viruses. Here, we describe three adjuvants that enhance the induction of stalk-directed antibodies against heterologous and heterosubtypic influenza viruses when administered with chimeric HA proteins. Addavax, an MF59-like nanoemulsion, poly(I:C), and an RNA hairpin derived from Sendai virus (SeV) Cantell were efficacious intramuscularly. The SeV RNA and poly(I:C) also proved to be effective respiratory mucosal adjuvants. Although the quantity and quality of antibodies induced by the adjuvants varied, immunized mice demonstrated comparable levels of protection against challenge with influenza A viruses on the basis of HA stalk reactivity. Finally, we present that intranasally, but not intramuscularly, administered chimeric HA proteins induce mucosal IgA antibodies directed at the HA stalk.
format	article
author	Peter H Goff Dirk Eggink Christopher W Seibert Rong Hai Luis Martínez-Gil Florian Krammer Peter Palese
author_facet	Peter H Goff Dirk Eggink Christopher W Seibert Rong Hai Luis Martínez-Gil Florian Krammer Peter Palese
author_sort	Peter H Goff
title	Adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies.
title_short	Adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies.
title_full	Adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies.
title_fullStr	Adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies.
title_full_unstemmed	Adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies.
title_sort	adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/512980efeaaa4d36b2f9273302d7b0ea
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Adjuvants and immunization strategies to induce influenza virus hemagglutinin stalk antibodies.

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