Molecular relatedness of methicillin-resistant S. aureus isolates from staff, environment and pets at University Veterinary Hospital in Malaysia.

Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a problem in veterinary medicine and is no longer considered as a mere nosocomial pathogen. We studied the occurrence of MRSA in veterinary personnel, cats and dogs and the environmental premises in University Veterinary Hospital (UVH...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Erkihun Aklilu, Zunita Zakaria, Latiffah Hassan, Chen Hui Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/513882032b094419b9ce56289504497f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:513882032b094419b9ce56289504497f
record_format	dspace
spelling	oai:doaj.org-article:513882032b094419b9ce56289504497f2021-11-18T07:07:47ZMolecular relatedness of methicillin-resistant S. aureus isolates from staff, environment and pets at University Veterinary Hospital in Malaysia.1932-620310.1371/journal.pone.0043329https://doaj.org/article/513882032b094419b9ce56289504497f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22937034/?tool=EBIhttps://doaj.org/toc/1932-6203Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a problem in veterinary medicine and is no longer considered as a mere nosocomial pathogen. We studied the occurrence of MRSA in veterinary personnel, cats and dogs and the environmental premises in University Veterinary Hospital (UVH). We found the prevalence of MRSA as follows: UVH 2/28 (7.1%) staff, 8/100 (8%) of the pets [5/50 (10%) of the dogs and 3/50 (6%) of the cats)], and 9/28 (4.5%) of the environmental samples. Antibiotic sensitivity tests (AST) show multi-resistance characteristics of the MRSA and the minimum inhibitory concentration (MIC) values for the isolates ranged from 1.5 µg to >256 µg/ml. Molecular typing by using multi-locus sequence typing (MLST), staphylococcal protein A typing (spa typing) and pulsed-field gel electrophoresis (PFGE) was conducted and the results from MLST indicated that an isolate from a veterinary personnel (PG21), typed as ST1241 belonged to the same clonal complex (CC) as the two isolates from two dogs (DG16 and DG20), both being typed as ST59. The PFGE results revealed that the two isolates from two veterinary personnel, PG21 and PG16 belonged to closely related MRSA strains with isolates from dog (DG36) and from environmental surface (EV100) respectively. The fact that PFGE revealed close similarity between isolates from humans, a dog and environmental surfaces indicates the possibility for either of them to be the source of MRSA and the potential routes and risks of spread.Erkihun AkliluZunita ZakariaLatiffah HassanChen Hui ChengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43329 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erkihun Aklilu Zunita Zakaria Latiffah Hassan Chen Hui Cheng Molecular relatedness of methicillin-resistant S. aureus isolates from staff, environment and pets at University Veterinary Hospital in Malaysia.
description	Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a problem in veterinary medicine and is no longer considered as a mere nosocomial pathogen. We studied the occurrence of MRSA in veterinary personnel, cats and dogs and the environmental premises in University Veterinary Hospital (UVH). We found the prevalence of MRSA as follows: UVH 2/28 (7.1%) staff, 8/100 (8%) of the pets [5/50 (10%) of the dogs and 3/50 (6%) of the cats)], and 9/28 (4.5%) of the environmental samples. Antibiotic sensitivity tests (AST) show multi-resistance characteristics of the MRSA and the minimum inhibitory concentration (MIC) values for the isolates ranged from 1.5 µg to >256 µg/ml. Molecular typing by using multi-locus sequence typing (MLST), staphylococcal protein A typing (spa typing) and pulsed-field gel electrophoresis (PFGE) was conducted and the results from MLST indicated that an isolate from a veterinary personnel (PG21), typed as ST1241 belonged to the same clonal complex (CC) as the two isolates from two dogs (DG16 and DG20), both being typed as ST59. The PFGE results revealed that the two isolates from two veterinary personnel, PG21 and PG16 belonged to closely related MRSA strains with isolates from dog (DG36) and from environmental surface (EV100) respectively. The fact that PFGE revealed close similarity between isolates from humans, a dog and environmental surfaces indicates the possibility for either of them to be the source of MRSA and the potential routes and risks of spread.
format	article
author	Erkihun Aklilu Zunita Zakaria Latiffah Hassan Chen Hui Cheng
author_facet	Erkihun Aklilu Zunita Zakaria Latiffah Hassan Chen Hui Cheng
author_sort	Erkihun Aklilu
title	Molecular relatedness of methicillin-resistant S. aureus isolates from staff, environment and pets at University Veterinary Hospital in Malaysia.
title_short	Molecular relatedness of methicillin-resistant S. aureus isolates from staff, environment and pets at University Veterinary Hospital in Malaysia.
title_full	Molecular relatedness of methicillin-resistant S. aureus isolates from staff, environment and pets at University Veterinary Hospital in Malaysia.
title_fullStr	Molecular relatedness of methicillin-resistant S. aureus isolates from staff, environment and pets at University Veterinary Hospital in Malaysia.
title_full_unstemmed	Molecular relatedness of methicillin-resistant S. aureus isolates from staff, environment and pets at University Veterinary Hospital in Malaysia.
title_sort	molecular relatedness of methicillin-resistant s. aureus isolates from staff, environment and pets at university veterinary hospital in malaysia.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/513882032b094419b9ce56289504497f
work_keys_str_mv	AT erkihunaklilu molecularrelatednessofmethicillinresistantsaureusisolatesfromstaffenvironmentandpetsatuniversityveterinaryhospitalinmalaysia AT zunitazakaria molecularrelatednessofmethicillinresistantsaureusisolatesfromstaffenvironmentandpetsatuniversityveterinaryhospitalinmalaysia AT latiffahhassan molecularrelatednessofmethicillinresistantsaureusisolatesfromstaffenvironmentandpetsatuniversityveterinaryhospitalinmalaysia AT chenhuicheng molecularrelatednessofmethicillinresistantsaureusisolatesfromstaffenvironmentandpetsatuniversityveterinaryhospitalinmalaysia
_version_	1718423913930686464

Molecular relatedness of methicillin-resistant S. aureus isolates from staff, environment and pets at University Veterinary Hospital in Malaysia.

Ejemplares similares