Increased fibroblast functionality on CNN2-loaded titania nanotubes

Hongbo Wei*, Shuyi Wu*, Zhihong Feng, Wei Zhou, Yan Dong, Guofeng Wu, Shizhu Bai, Yimin Zhao Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi&#39;an, People&#39;s Republic of China *These authors contributed equally to this workAbstract: Inf...

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Autores principales:	Wei HB, Wu SY, Feng ZH, Zhou W, Dong Y, Wu GF, Bai SZ, Zhao YM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/51390cea3c4a4c03bf0d9be0dd4bb277
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spelling	oai:doaj.org-article:51390cea3c4a4c03bf0d9be0dd4bb2772021-12-02T04:58:03ZIncreased fibroblast functionality on CNN2-loaded titania nanotubes1176-91141178-2013https://doaj.org/article/51390cea3c4a4c03bf0d9be0dd4bb2772012-02-01T00:00:00Zhttp://www.dovepress.com/increased-fibroblast-functionality-on-cnn2-loaded-titania-nanotubes-a9336https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Hongbo Wei, Shuyi Wu, Zhihong Feng, Wei Zhou, Yan Dong, Guofeng Wu, Shizhu Bai, Yimin Zhao Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi&#39;an, People&#39;s Republic of China *These authors contributed equally to this workAbstract: Infection and epithelial downgrowth are major problems associated with maxillofacial percutaneous implants. These complications are mainly due to the improper closure of the implant&ndash;skin interface. Therefore, designing a percutaneous implant that better promotes the formation of a stable soft tissue biologic seal around percutaneous sites is highly desirable. Additionally, the fibroblast has been proven to play an important role in the formation of biologic seals. In this study, titania nanotubes were filled with 11.2 kDa C-terminal CCN2 (connective tissue growth factor) fragment, which could exert full CCN2 activity to increase the biological functionality of fibroblasts. This drug delivery system was fabricated on a titanium implant surface. CCN2 was loaded into anodized titania nanotubes using a simplified lyophilization method and the loading efficiency was approximately 80%. Then, the release kinetics of CCN2 from these nanotubes was investigated. Furthermore, the influence of CCN2-loaded titania nanotubes on fibroblast functionality was examined. The results revealed increased fibroblast adhesion at 0.25, 0.5, 1, 2, 4, and 24 hours, increased fibroblast viability over the course of 5 days, as well as enhanced actin cytoskeleton organization on CCN2-loaded titania nanotubes surfaces compared to uncoated, unmodified counterparts. Therefore, the results from this in vitro study demonstrate that CCN2-loaded titania nanotubes have the ability to increase fibroblast functionality and should be further studied as a method of promoting the formation of a stable soft tissue biologic seal around percutaneous sites.Keywords: anodization, titania nanotubes, adhesion, connective tissue growth factor, fibroblastWei HBWu SYFeng ZHZhou WDong YWu GFBai SZZhao YMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1091-1100 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wei HB Wu SY Feng ZH Zhou W Dong Y Wu GF Bai SZ Zhao YM Increased fibroblast functionality on CNN2-loaded titania nanotubes
description	Hongbo Wei, Shuyi Wu, Zhihong Feng, Wei Zhou, Yan Dong, Guofeng Wu, Shizhu Bai, Yimin Zhao Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi&#39;an, People&#39;s Republic of China *These authors contributed equally to this workAbstract: Infection and epithelial downgrowth are major problems associated with maxillofacial percutaneous implants. These complications are mainly due to the improper closure of the implant&ndash;skin interface. Therefore, designing a percutaneous implant that better promotes the formation of a stable soft tissue biologic seal around percutaneous sites is highly desirable. Additionally, the fibroblast has been proven to play an important role in the formation of biologic seals. In this study, titania nanotubes were filled with 11.2 kDa C-terminal CCN2 (connective tissue growth factor) fragment, which could exert full CCN2 activity to increase the biological functionality of fibroblasts. This drug delivery system was fabricated on a titanium implant surface. CCN2 was loaded into anodized titania nanotubes using a simplified lyophilization method and the loading efficiency was approximately 80%. Then, the release kinetics of CCN2 from these nanotubes was investigated. Furthermore, the influence of CCN2-loaded titania nanotubes on fibroblast functionality was examined. The results revealed increased fibroblast adhesion at 0.25, 0.5, 1, 2, 4, and 24 hours, increased fibroblast viability over the course of 5 days, as well as enhanced actin cytoskeleton organization on CCN2-loaded titania nanotubes surfaces compared to uncoated, unmodified counterparts. Therefore, the results from this in vitro study demonstrate that CCN2-loaded titania nanotubes have the ability to increase fibroblast functionality and should be further studied as a method of promoting the formation of a stable soft tissue biologic seal around percutaneous sites.Keywords: anodization, titania nanotubes, adhesion, connective tissue growth factor, fibroblast
format	article
author	Wei HB Wu SY Feng ZH Zhou W Dong Y Wu GF Bai SZ Zhao YM
author_facet	Wei HB Wu SY Feng ZH Zhou W Dong Y Wu GF Bai SZ Zhao YM
author_sort	Wei HB
title	Increased fibroblast functionality on CNN2-loaded titania nanotubes
title_short	Increased fibroblast functionality on CNN2-loaded titania nanotubes
title_full	Increased fibroblast functionality on CNN2-loaded titania nanotubes
title_fullStr	Increased fibroblast functionality on CNN2-loaded titania nanotubes
title_full_unstemmed	Increased fibroblast functionality on CNN2-loaded titania nanotubes
title_sort	increased fibroblast functionality on cnn2-loaded titania nanotubes
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/51390cea3c4a4c03bf0d9be0dd4bb277
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Increased fibroblast functionality on CNN2-loaded titania nanotubes

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