Enpp2 Expression by Dendritic Cells Is a Key Regulator in Migration

Enpp2 is an enzyme that catalyzes the conversion of lysophosphatidylcholine (LPC) to lysophosphatidic acid (LPA), which exhibits a wide variety of biological functions. Here, we examined the biological effects of Enpp2 on dendritic cells (DCs), which are specialized antigen-presenting cells (APCs) c...

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Autores principales:	Jun-Ho Lee, So-Yeon Choi, Soo-Yeoun Park, Nam-Chul Jung, Kyung-Eun Noh, Ji-Hee Nam, Ji-Soo Oh, Hyun-Ji Choi, Ji-Su Jang, Ji-Young Yoo, Jie-Young Song, Han Geuk Seo, Dae-Seog Lim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Enpp2 dendritic cells migration Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/513bdf9b0c1c48bb8aa4ec6be5b96d04
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spelling	oai:doaj.org-article:513bdf9b0c1c48bb8aa4ec6be5b96d042021-11-25T16:51:28ZEnpp2 Expression by Dendritic Cells Is a Key Regulator in Migration10.3390/biomedicines91117272227-9059https://doaj.org/article/513bdf9b0c1c48bb8aa4ec6be5b96d042021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1727https://doaj.org/toc/2227-9059Enpp2 is an enzyme that catalyzes the conversion of lysophosphatidylcholine (LPC) to lysophosphatidic acid (LPA), which exhibits a wide variety of biological functions. Here, we examined the biological effects of Enpp2 on dendritic cells (DCs), which are specialized antigen-presenting cells (APCs) characterized by their ability to migrate into secondary lymphoid organs and activate naïve T-cells. DCs were generated from bone marrow progenitors obtained from C57BL/6 mice. Enpp2 levels in DCs were regulated using small interfering (si)RNA or recombinant Enpp2. Expression of Enpp2 in LPS-stimulated mature (m)DCs was high, however, knocking down Enpp2 inhibited mDC function. In addition, the migratory capacity of mDCs increased after treatment with rmEnpp2; this phenomenon was mediated via the RhoA-mediated signaling pathway. Enpp2-treated mDCs showed a markedly increased capacity to migrate to lymph nodes in vivo. These findings strongly suggest that Enpp2 is necessary for mDC migration capacity, thereby increasing our understanding of DC biology. We postulate that regulating Enpp2 improves DC migration to lymph nodes, thus improving the effectiveness of cancer vaccines based on DC.Jun-Ho LeeSo-Yeon ChoiSoo-Yeoun ParkNam-Chul JungKyung-Eun NohJi-Hee NamJi-Soo OhHyun-Ji ChoiJi-Su JangJi-Young YooJie-Young SongHan Geuk SeoDae-Seog LimMDPI AGarticleEnpp2dendritic cellsmigrationBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1727, p 1727 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Enpp2 dendritic cells migration Biology (General) QH301-705.5
spellingShingle	Enpp2 dendritic cells migration Biology (General) QH301-705.5 Jun-Ho Lee So-Yeon Choi Soo-Yeoun Park Nam-Chul Jung Kyung-Eun Noh Ji-Hee Nam Ji-Soo Oh Hyun-Ji Choi Ji-Su Jang Ji-Young Yoo Jie-Young Song Han Geuk Seo Dae-Seog Lim Enpp2 Expression by Dendritic Cells Is a Key Regulator in Migration
description	Enpp2 is an enzyme that catalyzes the conversion of lysophosphatidylcholine (LPC) to lysophosphatidic acid (LPA), which exhibits a wide variety of biological functions. Here, we examined the biological effects of Enpp2 on dendritic cells (DCs), which are specialized antigen-presenting cells (APCs) characterized by their ability to migrate into secondary lymphoid organs and activate naïve T-cells. DCs were generated from bone marrow progenitors obtained from C57BL/6 mice. Enpp2 levels in DCs were regulated using small interfering (si)RNA or recombinant Enpp2. Expression of Enpp2 in LPS-stimulated mature (m)DCs was high, however, knocking down Enpp2 inhibited mDC function. In addition, the migratory capacity of mDCs increased after treatment with rmEnpp2; this phenomenon was mediated via the RhoA-mediated signaling pathway. Enpp2-treated mDCs showed a markedly increased capacity to migrate to lymph nodes in vivo. These findings strongly suggest that Enpp2 is necessary for mDC migration capacity, thereby increasing our understanding of DC biology. We postulate that regulating Enpp2 improves DC migration to lymph nodes, thus improving the effectiveness of cancer vaccines based on DC.
format	article
author	Jun-Ho Lee So-Yeon Choi Soo-Yeoun Park Nam-Chul Jung Kyung-Eun Noh Ji-Hee Nam Ji-Soo Oh Hyun-Ji Choi Ji-Su Jang Ji-Young Yoo Jie-Young Song Han Geuk Seo Dae-Seog Lim
author_facet	Jun-Ho Lee So-Yeon Choi Soo-Yeoun Park Nam-Chul Jung Kyung-Eun Noh Ji-Hee Nam Ji-Soo Oh Hyun-Ji Choi Ji-Su Jang Ji-Young Yoo Jie-Young Song Han Geuk Seo Dae-Seog Lim
author_sort	Jun-Ho Lee
title	Enpp2 Expression by Dendritic Cells Is a Key Regulator in Migration
title_short	Enpp2 Expression by Dendritic Cells Is a Key Regulator in Migration
title_full	Enpp2 Expression by Dendritic Cells Is a Key Regulator in Migration
title_fullStr	Enpp2 Expression by Dendritic Cells Is a Key Regulator in Migration
title_full_unstemmed	Enpp2 Expression by Dendritic Cells Is a Key Regulator in Migration
title_sort	enpp2 expression by dendritic cells is a key regulator in migration
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/513bdf9b0c1c48bb8aa4ec6be5b96d04
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Enpp2 Expression by Dendritic Cells Is a Key Regulator in Migration

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