In vitro demodicidal activity of commercial lid hygiene products

Alan G KabatPennsylvania College of Optometry, Salus University, Elkins Park, PA 19027, USAPurpose: Demodex folliculorum is a ubiquitous mite that can infest the eyelash follicles. Two commercial lid hygiene products have asserted their effectiveness in killing Demodex mites, yet there has never bee...

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Autor principal:	Kabat AG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Demodex blepharitis lid hygiene tea tree oil terpinen-4-ol (T4O) hypochlorous acid (HOCl) Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/513ec2ff54e44cec983037762ad4c270
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spelling	oai:doaj.org-article:513ec2ff54e44cec983037762ad4c2702021-12-02T07:46:19ZIn vitro demodicidal activity of commercial lid hygiene products1177-5483https://doaj.org/article/513ec2ff54e44cec983037762ad4c2702019-08-01T00:00:00Zhttps://www.dovepress.com/in-vitro-demodicidal-activity-of-commercial-lid-hygiene-products-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Alan G KabatPennsylvania College of Optometry, Salus University, Elkins Park, PA 19027, USAPurpose: Demodex folliculorum is a ubiquitous mite that can infest the eyelash follicles. Two commercial lid hygiene products have asserted their effectiveness in killing Demodex mites, yet there has never been a comparative trial between these two products. This study evaluated the demodicidal activity of 0.01% hypochlorous acid (HOCl) solution (Avenova; NovaBay Pharmaceuticals; Emeryville, CA) and 4% terpinen-4-ol (T4O) solution (Cliradex, Bio-Tissue; Miami, FL) in comparison to mineral oil (MO), a negative control.Methods: Live Demodex mites were obtained from volunteers. Samples were immersed in 1–2 drops of test solution: 0.01% HOCl, 4% T4O, or 100% MO. Samples were observed under the microscope every 10 mins for up to 90 mins. Kill time was defined as the elapsed time between the addition of test solution and all cessation of movement of the body, legs, mouth and pedipalps for a minimum of 60 seconds.Results: T4O demonstrated a mean kill time of 40±0.0 mins. HOCl had a mean kill time of 87.86±4.23 mins, with 79% of samples surviving the full 90 mins. In the MO group, all samples survived through the 90 min mark. Kill time was statistically significant in favor of T4O as compared to HOCl (p=0.0005). There was no statistically significant difference in kill time between HOCl and MO (p=0.25).Conclusion: 4% T4O effectively killed all adult mite samples within 40 mins of exposure. In contrast, the demodicidal activity of 0.01% HOCl was minimal, and comparatively similar to 100% MO.Keywords: Demodex, blepharitis, lid hygiene, tea tree oil, terpinen-4-ol, hypochlorous acidKabat AGDove Medical PressarticleDemodexblepharitislid hygienetea tree oilterpinen-4-ol (T4O)hypochlorous acid (HOCl)OphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 13, Pp 1493-1497 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Demodex blepharitis lid hygiene tea tree oil terpinen-4-ol (T4O) hypochlorous acid (HOCl) Ophthalmology RE1-994
spellingShingle	Demodex blepharitis lid hygiene tea tree oil terpinen-4-ol (T4O) hypochlorous acid (HOCl) Ophthalmology RE1-994 Kabat AG In vitro demodicidal activity of commercial lid hygiene products
description	Alan G KabatPennsylvania College of Optometry, Salus University, Elkins Park, PA 19027, USAPurpose: Demodex folliculorum is a ubiquitous mite that can infest the eyelash follicles. Two commercial lid hygiene products have asserted their effectiveness in killing Demodex mites, yet there has never been a comparative trial between these two products. This study evaluated the demodicidal activity of 0.01% hypochlorous acid (HOCl) solution (Avenova; NovaBay Pharmaceuticals; Emeryville, CA) and 4% terpinen-4-ol (T4O) solution (Cliradex, Bio-Tissue; Miami, FL) in comparison to mineral oil (MO), a negative control.Methods: Live Demodex mites were obtained from volunteers. Samples were immersed in 1–2 drops of test solution: 0.01% HOCl, 4% T4O, or 100% MO. Samples were observed under the microscope every 10 mins for up to 90 mins. Kill time was defined as the elapsed time between the addition of test solution and all cessation of movement of the body, legs, mouth and pedipalps for a minimum of 60 seconds.Results: T4O demonstrated a mean kill time of 40±0.0 mins. HOCl had a mean kill time of 87.86±4.23 mins, with 79% of samples surviving the full 90 mins. In the MO group, all samples survived through the 90 min mark. Kill time was statistically significant in favor of T4O as compared to HOCl (p=0.0005). There was no statistically significant difference in kill time between HOCl and MO (p=0.25).Conclusion: 4% T4O effectively killed all adult mite samples within 40 mins of exposure. In contrast, the demodicidal activity of 0.01% HOCl was minimal, and comparatively similar to 100% MO.Keywords: Demodex, blepharitis, lid hygiene, tea tree oil, terpinen-4-ol, hypochlorous acid
format	article
author	Kabat AG
author_facet	Kabat AG
author_sort	Kabat AG
title	In vitro demodicidal activity of commercial lid hygiene products
title_short	In vitro demodicidal activity of commercial lid hygiene products
title_full	In vitro demodicidal activity of commercial lid hygiene products
title_fullStr	In vitro demodicidal activity of commercial lid hygiene products
title_full_unstemmed	In vitro demodicidal activity of commercial lid hygiene products
title_sort	in vitro demodicidal activity of commercial lid hygiene products
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/513ec2ff54e44cec983037762ad4c270
work_keys_str_mv	AT kabatag invitrodemodicidalactivityofcommerciallidhygieneproducts
_version_	1718399153812275200

In vitro demodicidal activity of commercial lid hygiene products

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