Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang

Abstract Background Sanghuangporus sanghuang is a well-known traditional medicinal mushroom associated with mulberry. Despite the properties of this mushroom being known for many years, the regulatory mechanisms of bioactive compound biosynthesis in this medicinal mushroom are still unclear. Lysine...

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Autores principales:	Tong Wang, Guangyuan Wang, Guoli Zhang, Ranran Hou, Liwei Zhou, Xuemei Tian
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Malonylation Posttranslational modification (PTM) Malonylproteome Sanghuangporus sanghuang Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/513f8084b27747938b234b4de6f4fadd
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spelling	oai:doaj.org-article:513f8084b27747938b234b4de6f4fadd2021-11-21T12:26:24ZSystematic analysis of the lysine malonylome in Sanghuangporus sanghuang10.1186/s12864-021-08120-01471-2164https://doaj.org/article/513f8084b27747938b234b4de6f4fadd2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08120-0https://doaj.org/toc/1471-2164Abstract Background Sanghuangporus sanghuang is a well-known traditional medicinal mushroom associated with mulberry. Despite the properties of this mushroom being known for many years, the regulatory mechanisms of bioactive compound biosynthesis in this medicinal mushroom are still unclear. Lysine malonylation is a posttranslational modification that has many critical functions in various aspects of cell metabolism. However, at present we do not know its role in S. sanghuang. In this study, a global investigation of the lysine malonylome in S. sanghuang was therefore carried out. Results In total, 714 malonyl modification sites were matched to 255 different proteins. The analysis indicated that malonyl modifications were involved in a wide range of cellular functions and displayed a distinct subcellular localization. Bioinformatics analysis indicated that malonylated proteins were engaged in different metabolic pathways, including glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and the tricarboxylic acid (TCA) cycle. Notably, a total of 26 enzymes related to triterpene and polysaccharide biosynthesis were found to be malonylated, indicating an indispensable role of lysine malonylation in bioactive compound biosynthesis in S. sanghuang. Conclusions These findings suggest that malonylation is associated with many metabolic pathways, particularly the metabolism of the bioactive compounds triterpene and polysaccharide. This paper represents the first comprehensive survey of malonylation in S. sanghuang and provides important data for further study on the physiological function of lysine malonylation in S. sanghuang and other medicinal mushrooms.Tong WangGuangyuan WangGuoli ZhangRanran HouLiwei ZhouXuemei TianBMCarticleMalonylationPosttranslational modification (PTM)MalonylproteomeSanghuangporus sanghuangBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Malonylation Posttranslational modification (PTM) Malonylproteome Sanghuangporus sanghuang Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Malonylation Posttranslational modification (PTM) Malonylproteome Sanghuangporus sanghuang Biotechnology TP248.13-248.65 Genetics QH426-470 Tong Wang Guangyuan Wang Guoli Zhang Ranran Hou Liwei Zhou Xuemei Tian Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang
description	Abstract Background Sanghuangporus sanghuang is a well-known traditional medicinal mushroom associated with mulberry. Despite the properties of this mushroom being known for many years, the regulatory mechanisms of bioactive compound biosynthesis in this medicinal mushroom are still unclear. Lysine malonylation is a posttranslational modification that has many critical functions in various aspects of cell metabolism. However, at present we do not know its role in S. sanghuang. In this study, a global investigation of the lysine malonylome in S. sanghuang was therefore carried out. Results In total, 714 malonyl modification sites were matched to 255 different proteins. The analysis indicated that malonyl modifications were involved in a wide range of cellular functions and displayed a distinct subcellular localization. Bioinformatics analysis indicated that malonylated proteins were engaged in different metabolic pathways, including glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and the tricarboxylic acid (TCA) cycle. Notably, a total of 26 enzymes related to triterpene and polysaccharide biosynthesis were found to be malonylated, indicating an indispensable role of lysine malonylation in bioactive compound biosynthesis in S. sanghuang. Conclusions These findings suggest that malonylation is associated with many metabolic pathways, particularly the metabolism of the bioactive compounds triterpene and polysaccharide. This paper represents the first comprehensive survey of malonylation in S. sanghuang and provides important data for further study on the physiological function of lysine malonylation in S. sanghuang and other medicinal mushrooms.
format	article
author	Tong Wang Guangyuan Wang Guoli Zhang Ranran Hou Liwei Zhou Xuemei Tian
author_facet	Tong Wang Guangyuan Wang Guoli Zhang Ranran Hou Liwei Zhou Xuemei Tian
author_sort	Tong Wang
title	Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang
title_short	Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang
title_full	Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang
title_fullStr	Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang
title_full_unstemmed	Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang
title_sort	systematic analysis of the lysine malonylome in sanghuangporus sanghuang
publisher	BMC
publishDate	2021
url	https://doaj.org/article/513f8084b27747938b234b4de6f4fadd
work_keys_str_mv	AT tongwang systematicanalysisofthelysinemalonylomeinsanghuangporussanghuang AT guangyuanwang systematicanalysisofthelysinemalonylomeinsanghuangporussanghuang AT guolizhang systematicanalysisofthelysinemalonylomeinsanghuangporussanghuang AT ranranhou systematicanalysisofthelysinemalonylomeinsanghuangporussanghuang AT liweizhou systematicanalysisofthelysinemalonylomeinsanghuangporussanghuang AT xuemeitian systematicanalysisofthelysinemalonylomeinsanghuangporussanghuang
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Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang

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