Plasmodium falciparum antigens on the surface of the gametocyte-infected erythrocyte.

<h4>Background</h4>The asexual blood stages of the human malaria parasite Plasmodium falciparum produce highly immunogenic polymorphic antigens that are expressed on the surface of the host cell. In contrast, few studies have examined the surface of the gametocyte-infected erythrocyte.&l...

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Autores principales: Maha Saeed, Will Roeffen, Neal Alexander, Christopher J Drakeley, Geoffrey A T Targett, Colin J Sutherland
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Publicado: Public Library of Science (PLoS) 2008
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spelling oai:doaj.org-article:5141fbd8546e421c8806f6476ae350642021-12-02T20:12:21ZPlasmodium falciparum antigens on the surface of the gametocyte-infected erythrocyte.1932-620310.1371/journal.pone.0002280https://doaj.org/article/5141fbd8546e421c8806f6476ae350642008-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18509532/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The asexual blood stages of the human malaria parasite Plasmodium falciparum produce highly immunogenic polymorphic antigens that are expressed on the surface of the host cell. In contrast, few studies have examined the surface of the gametocyte-infected erythrocyte.<h4>Methodology/principal findings</h4>We used flow cytometry to detect antibodies recognising the surface of live cultured erythrocytes infected with gametocytes of P. falciparum strain 3D7 in the plasma of 200 Gambian children. The majority of children had been identified as carrying gametocytes after treatment for malaria, and each donated blood for mosquito-feeding experiments. None of the plasma recognised the surface of erythrocytes infected with developmental stages of gametocytes (I-IV), but 66 of 194 (34.0%) plasma contained IgG that recognised the surface of erythrocytes infected with mature (stage V) gametocytes. Thirty-four (17.0%) of 200 plasma tested recognised erythrocytes infected with trophozoites and schizonts, but there was no association with recognition of the surface of gametocyte-infected erythrocytes (odds ratio 1.08, 95% C.I. 0.434-2.57; P = 0.851). Plasma antibodies with the ability to recognise gametocyte surface antigens (GSA) were associated with the presence of antibodies that recognise the gamete antigen Pfs 230, but not Pfs48/45. Antibodies recognising GSA were associated with donors having lower gametocyte densities 4 weeks after antimalarial treatment.<h4>Conclusions/significance</h4>We provide evidence that GSA are distinct from antigens detected on the surface of asexual 3D7 parasites. Our findings suggest a novel strategy for the development of transmission-blocking vaccines.Maha SaeedWill RoeffenNeal AlexanderChristopher J DrakeleyGeoffrey A T TargettColin J SutherlandPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 5, p e2280 (2008)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Maha Saeed
Will Roeffen
Neal Alexander
Christopher J Drakeley
Geoffrey A T Targett
Colin J Sutherland
Plasmodium falciparum antigens on the surface of the gametocyte-infected erythrocyte.
description <h4>Background</h4>The asexual blood stages of the human malaria parasite Plasmodium falciparum produce highly immunogenic polymorphic antigens that are expressed on the surface of the host cell. In contrast, few studies have examined the surface of the gametocyte-infected erythrocyte.<h4>Methodology/principal findings</h4>We used flow cytometry to detect antibodies recognising the surface of live cultured erythrocytes infected with gametocytes of P. falciparum strain 3D7 in the plasma of 200 Gambian children. The majority of children had been identified as carrying gametocytes after treatment for malaria, and each donated blood for mosquito-feeding experiments. None of the plasma recognised the surface of erythrocytes infected with developmental stages of gametocytes (I-IV), but 66 of 194 (34.0%) plasma contained IgG that recognised the surface of erythrocytes infected with mature (stage V) gametocytes. Thirty-four (17.0%) of 200 plasma tested recognised erythrocytes infected with trophozoites and schizonts, but there was no association with recognition of the surface of gametocyte-infected erythrocytes (odds ratio 1.08, 95% C.I. 0.434-2.57; P = 0.851). Plasma antibodies with the ability to recognise gametocyte surface antigens (GSA) were associated with the presence of antibodies that recognise the gamete antigen Pfs 230, but not Pfs48/45. Antibodies recognising GSA were associated with donors having lower gametocyte densities 4 weeks after antimalarial treatment.<h4>Conclusions/significance</h4>We provide evidence that GSA are distinct from antigens detected on the surface of asexual 3D7 parasites. Our findings suggest a novel strategy for the development of transmission-blocking vaccines.
format article
author Maha Saeed
Will Roeffen
Neal Alexander
Christopher J Drakeley
Geoffrey A T Targett
Colin J Sutherland
author_facet Maha Saeed
Will Roeffen
Neal Alexander
Christopher J Drakeley
Geoffrey A T Targett
Colin J Sutherland
author_sort Maha Saeed
title Plasmodium falciparum antigens on the surface of the gametocyte-infected erythrocyte.
title_short Plasmodium falciparum antigens on the surface of the gametocyte-infected erythrocyte.
title_full Plasmodium falciparum antigens on the surface of the gametocyte-infected erythrocyte.
title_fullStr Plasmodium falciparum antigens on the surface of the gametocyte-infected erythrocyte.
title_full_unstemmed Plasmodium falciparum antigens on the surface of the gametocyte-infected erythrocyte.
title_sort plasmodium falciparum antigens on the surface of the gametocyte-infected erythrocyte.
publisher Public Library of Science (PLoS)
publishDate 2008
url https://doaj.org/article/5141fbd8546e421c8806f6476ae35064
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AT nealalexander plasmodiumfalciparumantigensonthesurfaceofthegametocyteinfectederythrocyte
AT christopherjdrakeley plasmodiumfalciparumantigensonthesurfaceofthegametocyteinfectederythrocyte
AT geoffreyattargett plasmodiumfalciparumantigensonthesurfaceofthegametocyteinfectederythrocyte
AT colinjsutherland plasmodiumfalciparumantigensonthesurfaceofthegametocyteinfectederythrocyte
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